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The map label for this gene is sucB [H]

Identifier: 225853360

GI number: 225853360

Start: 1871834

End: 1873060

Strand: Reverse

Name: sucB [H]

Synonym: BMEA_A1979

Alternate gene names: 225853360

Gene position: 1873060-1871834 (Counterclockwise)

Preceding gene: 225853361

Following gene: 225853359

Centisome position: 88.11

GC content: 61.29

Gene sequence:

>1227_bases
ATGGCTACCGAAATTCGCGTTCCCACGCTTGGGGAGTCCGTTACCGAGGCAACCATCGGAAAGTGGTTCAAGAAGGCTGG
TGAAGCCATTGCTGTCGATGAGCCGCTGGTGGAACTGGAAACCGACAAGGTGACGGTCGAAGTGCCCGCACCGGCTGCGG
GTGTTCTGGCCGAAATCACGGCCAAAGAAGGCGATACGGTCGAAGTGGGCGAACTGCTCGGCCAGATTTCCAGTGACGGG
GCAGCGGTTGCCGCCGCACCGGCCCAGAAGAAGGAGGAAGCCAAGCCTGCAGCCGCCGCTCCTGCTGCTGCACCGGTCGC
ATCGGCTTCGTCGGGCCCGGCCATGCAGCCGGCGCCGGCTGCTGCGAAGCTTCTGGCTGAAAGCGGTCTTTCCGCCGATC
AGGTCGAAGGTTCCGGCAAGCGCGGCCAGGTGCTCAAAGGTGACGTTCTCGAGGCCATCACGAAAGGCGTTTCGGCGGCT
CCTGCCGCTCCGGTTGCCGCGCGTCCGGCCTCCTCGGCTGATGACGCATCGCGCGAAGAGCGTGTGAAGATGACCCGTCT
GCGCCAGACCATTGCGCGCCGCCTGAAGGATGCCCAGAACACGGCTGCCATGCTCACCACCTTCAACGAGGTGGACATGT
CGGCAGTCATGGAGCTGCGTAACAAGTATAAGGACGTCTTTGAAAAGAAGCATGGCGTGAAGCTTGGCTTCATGGGCTTC
TTCACCAAGGCAGTGACGCATGCGCTGAAGGAAATCCCGGCTGTCAACGCCGAGATCGACGGCACGGACATCGTCTACAA
GAACTTCTGCCATGTCGGCATGGCTGTCGGCACCGACAAGGGCCTCGTCGTTCCGGTCATCCGCGATGCCGACCAGATGT
CGATTGCGGAAGTGGAAAAGGAACTGGCACGTCTCGCCAGGGCTGCGCGTGACGGCTCGCTTTCCATGGCCGATATGCAG
GGCGGCACCTTTACCATCACCAATGGCGGTGTTTACGGTTCGCTCATGTCCACGCCGATCCTCAATGCGCCGCAGTCGGG
CATTCTCGGTATGCACAAGATCCAGGAACGCCCGATGGTCGTCGGTGGCCAGATCGTGGTCCGCCCGATGATGTATCTGG
CGCTTTCCTACGATCACCGCATCGTGGACGGCAAGGAAGCCGTGACCTTCCTCGTGCGTGTGAAGGAAAGCCTGGAAGAT
CCGGAACGTCTGGTTCTCGATCTCTAG

Upstream 100 bases:

>100_bases
GCGCAGCTTGCCGCTTTCCTTGAGGATGCGCTTGGCTGATCGCGGCCTGCAGCAATTGAAATCGAACTTTAAAGACTGAA
GATCAACGGAAGAAGAAACC

Downstream 100 bases:

>100_bases
GGACAGAATATGGAGCCGGGTGTTCTCTCCGCGTCGCCTTTCCTGCCGCTCATTGGCTGGAGCGTTGTTCTGCTGGTGGT
GCATATCCTCTTGCAGAGCA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MATEIRVPTLGESVTEATIGKWFKKAGEAIAVDEPLVELETDKVTVEVPAPAAGVLAEITAKEGDTVEVGELLGQISSDG
AAVAAAPAQKKEEAKPAAAAPAAAPVASASSGPAMQPAPAAAKLLAESGLSADQVEGSGKRGQVLKGDVLEAITKGVSAA
PAAPVAARPASSADDASREERVKMTRLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMELRNKYKDVFEKKHGVKLGFMGF
FTKAVTHALKEIPAVNAEIDGTDIVYKNFCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELARLARAARDGSLSMADMQ
GGTFTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVVRPMMYLALSYDHRIVDGKEAVTFLVRVKESLED
PERLVLDL

Sequences:

>Translated_408_residues
MATEIRVPTLGESVTEATIGKWFKKAGEAIAVDEPLVELETDKVTVEVPAPAAGVLAEITAKEGDTVEVGELLGQISSDG
AAVAAAPAQKKEEAKPAAAAPAAAPVASASSGPAMQPAPAAAKLLAESGLSADQVEGSGKRGQVLKGDVLEAITKGVSAA
PAAPVAARPASSADDASREERVKMTRLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMELRNKYKDVFEKKHGVKLGFMGF
FTKAVTHALKEIPAVNAEIDGTDIVYKNFCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELARLARAARDGSLSMADMQ
GGTFTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVVRPMMYLALSYDHRIVDGKEAVTFLVRVKESLED
PERLVLDL
>Mature_407_residues
ATEIRVPTLGESVTEATIGKWFKKAGEAIAVDEPLVELETDKVTVEVPAPAAGVLAEITAKEGDTVEVGELLGQISSDGA
AVAAAPAQKKEEAKPAAAAPAAAPVASASSGPAMQPAPAAAKLLAESGLSADQVEGSGKRGQVLKGDVLEAITKGVSAAP
AAPVAARPASSADDASREERVKMTRLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMELRNKYKDVFEKKHGVKLGFMGFF
TKAVTHALKEIPAVNAEIDGTDIVYKNFCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELARLARAARDGSLSMADMQG
GTFTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVVRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDP
ERLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=248, Percent_Identity=55.241935483871, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=29.1111111111111, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=29.1111111111111, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=26.5734265734266, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=27.6102088167053, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=50.3685503685504, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=32, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=413, Percent_Identity=42.8571428571429, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=31.0657596371882, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=27.7647058823529, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=28.7581699346405, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=47.0731707317073, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=26.2582056892779, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=58.695652173913, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=29.4811320754717, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129315, Length=446, Percent_Identity=30.0448430493274, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24582497, Length=430, Percent_Identity=29.7674418604651, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42912; Mature: 42781

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVTEATIGKWFKKAGEAIAVDEPLVELETDKVTVEVPAPAAGVLAEIT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEECCEEEEEECCCCHHHHHHHH
AKEGDTVEVGELLGQISSDGAAVAAAPAQKKEEAKPAAAAPAAAPVASASSGPAMQPAPA
CCCCCEEHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
AAKLLAESGLSADQVEGSGKRGQVLKGDVLEAITKGVSAAPAAPVAARPASSADDASREE
HHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
RVKMTRLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMELRNKYKDVFEKKHGVKLGFMGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
FTKAVTHALKEIPAVNAEIDGTDIVYKNFCHVGMAVGTDKGLVVPVIRDADQMSIAEVEK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHH
ELARLARAARDGSLSMADMQGGTFTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPMV
HHHHHHHHHCCCCCEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCCHHHHHCCCEE
VGGQIVVRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
ECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
ATEIRVPTLGESVTEATIGKWFKKAGEAIAVDEPLVELETDKVTVEVPAPAAGVLAEIT
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEECCEEEEEECCCCHHHHHHHH
AKEGDTVEVGELLGQISSDGAAVAAAPAQKKEEAKPAAAAPAAAPVASASSGPAMQPAPA
CCCCCEEHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
AAKLLAESGLSADQVEGSGKRGQVLKGDVLEAITKGVSAAPAAPVAARPASSADDASREE
HHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
RVKMTRLRQTIARRLKDAQNTAAMLTTFNEVDMSAVMELRNKYKDVFEKKHGVKLGFMGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
FTKAVTHALKEIPAVNAEIDGTDIVYKNFCHVGMAVGTDKGLVVPVIRDADQMSIAEVEK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHH
ELARLARAARDGSLSMADMQGGTFTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPMV
HHHHHHHHHCCCCCEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCCHHHHHCCCEE
VGGQIVVRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLDL
ECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]