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The map label for this gene is leuC [H]

Identifier: 225853344

GI number: 225853344

Start: 1858649

End: 1860058

Strand: Reverse

Name: leuC [H]

Synonym: BMEA_A1961

Alternate gene names: 225853344

Gene position: 1860058-1858649 (Counterclockwise)

Preceding gene: 225853345

Following gene: 225853343

Centisome position: 87.5

GC content: 60.35

Gene sequence:

>1410_bases
ATGAGCGCGCCGCGTACACTTTATGACAAGATTTGGGATGACCATGTAGTGGATCAGCAGGAAGACGGCACCTGCCTCCT
TTATATTGATCGCCATCTTGTGCATGAAGTGACGAGCCCGCAGGCCTTTGAGGGGCTGCACATGGCAGGCCGCCCGGTGC
GCCACCCGGAAAAGACGCTTGCCGTGGTGGACCATAACGTTCCGACGTCGCCCGACCGCATCAATGGCATCCAGAACGAG
GAAAGCCGCATCCAGGTCGAGGCACTGGCCAGGAATGCCGCCGATTTCGGCGTCGAATATTATTCCGAACGCGACAAGCG
CCAGGGCATCGTGCATATCGTCGGGCCAGAGCAGGGCTTCACCCTGCCGGGCATGACCATCGTTTGCGGTGACAGCCACA
CTTCCACCCACGGCGCTTTCGGTGCGCTTGCACATGGCATCGGCACATCGGAGGTGGAGCATGTGCTCGCCACCCAGACG
CTGATCCAGAAAAAAGCCAAGAATATGCTGGTGCGCGTTGACGGCAAGTTGCCGGCGGGCGTGACCGCCAAGGATATTGT
TCTGGCGATCATTGGCGAGATCGGCACGGCAGGCGGCACCGGCTATGTCATCGAATATGCGGGCGAAGCCATTCGCTCGC
TGTCGATGGAAGGCCGCATGACGATCTGCAACATGTCGATCGAGGGCGGCGCGCGTGCCGGTCTTATCGCTCCCGACGAG
ACGACGTTTGAATATATCAAGGGCCGCCCGCGTGCTCCGCAGGGCGAAACGCTGGAACAGGCGATCAATTACTGGAAGAC
GCTGCATTCGGATGAAGGGGCGCATTTCGACAAGATCGTGACGCTCGACGCCGGCAGCCTGCCGCCCATCGTTTCCTGGG
GTTCTTCGCCGGAAGACGTCGTTTCCGTCACGGGGGTCGTGCCCAATCCGGACGATATCGCCGACGAAACCAAGCGTGCC
TCCAAGTGGCGTGCGCTCGACTATATGGGCCTGAAGCCCGGCACGAAAATCACGGATATCGCGGTTGATCGCGTCTTCAT
CGGTTCGTGCACCAATGGCCGCATCGAAGACCTTCGCGCTGCCGCCAAGGTCGTGGAAGGCAAGAAGGTTGCCCCGACCG
TCAATGCCATGATCGTTCCGGGTTCGGGTCTCGTAAAGGAACAGGCGGAAGCCGAAGGCCTGCACAAGATTTTCATTGAA
GCCGGTTTCGACTGGCGCGAGCCGGGCTGCTCCATGTGCCTTGCGATGAACGATGACCGGCTGAAGCCGGGCGAGCGTTG
CGCTTCGACCTCGAACCGCAATTTCGAGGGCCGTCAGGGCTTCAAGGGTCGTACGCATCTGGTATCGCCAGCAATGGCTG
CGGCGGCTGCAATTGCCGGTCACTTCGTCGATATTCGCGCGTGGAAGTGA

Upstream 100 bases:

>100_bases
TTACTGCGATACCCTGTTGCGGTTTCTGTTTCTTGACGAAAAGCGGTGGCAGGTGCATATGACAGCCTGGAATTAAGAAC
CGTTCAAAGGAACCCTCGCA

Downstream 100 bases:

>100_bases
GCACAACCCCGCATAAAGTTTGAAAAGACCGCCGGTTGATCCCGGCGGTCTTTTCGTTTTCCGTCACGACAGATCGGTCG
CACATATATCTTAACGAGGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNE
ESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRA
SKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK

Sequences:

>Translated_469_residues
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNE
ESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRA
SKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
>Mature_468_residues
SAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNEE
SRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDET
TFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRAS
KWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEA
GFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=382, Percent_Identity=28.0104712041885, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI8659555, Length=385, Percent_Identity=27.012987012987, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=26.3020833333333, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=67.5268817204301, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=28.808864265928, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081781, Length=348, Percent_Identity=25, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=30.1047120418848, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=30.1047120418848, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=30.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=28.992628992629, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=65.0423728813559, Blast_Score=618, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=426, Percent_Identity=25.5868544600939, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=474, Percent_Identity=28.0590717299578, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=487, Percent_Identity=26.4887063655031, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.7015706806283, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=26.7015706806283, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=26.7015706806283, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=27.0718232044199, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=30.1837270341207, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=28.2321899736148, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50693; Mature: 50562

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTL
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCHHHH
VSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTL
CCCHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCHHHH
VSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA