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The map label for this gene is dhaK [H]

Identifier: 225853095

GI number: 225853095

Start: 1592754

End: 1594838

Strand: Reverse

Name: dhaK [H]

Synonym: BMEA_A1678

Alternate gene names: 225853095

Gene position: 1594838-1592754 (Counterclockwise)

Preceding gene: 225853096

Following gene: 225853094

Centisome position: 75.03

GC content: 56.07

Gene sequence:

>2085_bases
ATGGCGCCACAGGGAAAAACAACCGCAGGTAAAATCGGACAGGCGGAACCAGCCGATGGCATTCCACTGCGCTATGGCGA
CGATCCATATGTCTGGGCTTGCTGGCTTTATTACGAAGACGGGCGCACGCAGGGCGAAATCGCCAACATCATGGGGATTT
CGCGGGCTACCGTAAACAGCTATCTCGCAGATGCCCGTAATCGAGGCATCGTCAATATCTCGATTGAACCTGCACGCCTC
GGTTCCCTTGCAATTGCACAGGAACTCAAGCACCATTTCGGATTGAGCGATTGTCTGGTGGTGCCCAATGATGACGGCGC
GCATCCCCTTATCGACCGGCTTGGGACGGCTGGTGCACAGGCGGTCACCAGGCTTCTGAAATCGGGAGACGTACTTGCCG
TCGCCTGGGGGCGGACAGTCCTTGCCATTGCCGAGAATACCTGCATATCCAATTTGCAGGATGTAACCGTAGTACAAGCA
ACCGGCGGGACGCGCGCCAGTTTTGCTTACACACCTGAATTGTGCGCGGCGGCCATGGCGCGCGCAACAAACGCACACCT
CGTCAACATCACGGCCCCCGCCATTGTATCCAGTGCAGACGTAAAGACGGCGATTACCGGGGAACCGATTATTGTTGAGC
AATTTCAGGTGTTGAACCATGCAAACAAGGCATTGTTTGGCATTTCATCGCTGCGGCCGAATTCAACAATCCATACCAGC
GGTTTCTTTGAAAATGTCGCGATACAGGATTACCTGGCCAAGAATGCCGTTGGCGTTGTAGCCGGGCGTTTCATCGATGC
TTATGGGCAACCGGTGGCCGGCCCGCTTGATGACAGGACCATTGGTATCACGCTCGACAGGCTGCGAATGATCGACATGC
GTATTGCCGTTGCGGGCGGCTTCGATAAGGTGCCGGCAATTCTTGCAGCCCTGCGGGGTGGTTATATCAATGTCCTGATT
ACCGATGCCGCGACAGGCCGTGGTATCCTCAATGCGGATGGCGTAACCGGGATCGACAGCAAGCTTTCCCAGAAGCTGCG
TGCAGAACCGGCCGCCAATGCATCCAGTCATTATCGTACGCATATCAAGAAATTTCTGAACAGCCCCGATGATGTCGTTG
AGGAAATGCTCGACGGCGCCACCAAAGCACATCGATCCTATCTCGTCCCCATCAATGGATCGAAACGCGCGCTCGTTGCA
CGCAATGGCCCCCGTGCGGGGAAAGTGGGACTGGTGATCGGGGGCGGTTCGGGGCATGAACCCTGCTTTCTGGGCTATGT
TGGCAAGGGTCTTGCCGATGCTGTAGCCATTGGCAATGTCTTTTCCTCACCACCGCCGGCACCGATCCTTGAATGCGCCA
GGGCGGCCTCGGGCGGAGAAGGTGTTCTCTTTGTCTATGGCAACTATGTCGGAGACGTCATGAATTTCGAGATGGCGGCG
GAAATGGCGCAGGAAGCTGCTATTCCGACACGTACCGTTCTGACGACAGACGACATATCGTCTTCCCCCATAGAGGATCG
CGATGGCCGTCGCGGCGTTGCGGGGAACTTTTTCATTTTCAAGGTAGCTGGCGCAGCTTGCGATCGTGGCATGTCGCTTA
AGGCATGTGAAGCGGTGACACGCAAGGCTAACCGGCGCACCTATACGATGGGCGTCGCACTGGAACCCTGTTCGCTGCCC
CAGACGCGGCGCCACAATTTTGAAATCGGCGCCGAAGAAATGGAAATCGGCATGGGCATCCATGGCGAACGCGGTGTCAT
CCGGGAAAAATTGTTGCCCGCCGACGCGATTGTCGATCACATCATGGACCGGATTTTCAGCGAGATGAATGCCTCGCCCG
GAGACCGGGTAGCGGTGCTCGTCAATTCGTTCGGTTCAACGCCCCTGATGGAACTCTACGTCCTGTTCAGACGTGTCGAG
CAACGCTTGAGCGCCAAGCACATAACAATCGAGGCCAACTGGGTGGGCCATTACTGTACGTCGCTGGACATGAACGGAGC
ATCCATATCCGTCATGCATCTCGATCAGGAATTATCCGATCTCTTATCGCACCCTTGCGATACGGCTTTTCTTCGGGTCA
ACTGA

Upstream 100 bases:

>100_bases
CTCCGGCAGGCAGGTGTCGGCAGAATGCAAACAAAAACATTGTATTGCAAGGCAACCCGGCAGATAGAAGGGAAAAGTCA
CGGGGATCAGTGCGAACAAG

Downstream 100 bases:

>100_bases
TTTGTCTCTCCAGCTTAATGCCGCTTCGGCGGTTTCATGAATTCCAGATTATCTGGGAGGTAAAAATGCAGGAAACCGCA
GCGCGTCATCTAGGCCGAAT

Product: glycerone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MAPQGKTTAGKIGQAEPADGIPLRYGDDPYVWACWLYYEDGRTQGEIANIMGISRATVNSYLADARNRGIVNISIEPARL
GSLAIAQELKHHFGLSDCLVVPNDDGAHPLIDRLGTAGAQAVTRLLKSGDVLAVAWGRTVLAIAENTCISNLQDVTVVQA
TGGTRASFAYTPELCAAAMARATNAHLVNITAPAIVSSADVKTAITGEPIIVEQFQVLNHANKALFGISSLRPNSTIHTS
GFFENVAIQDYLAKNAVGVVAGRFIDAYGQPVAGPLDDRTIGITLDRLRMIDMRIAVAGGFDKVPAILAALRGGYINVLI
TDAATGRGILNADGVTGIDSKLSQKLRAEPAANASSHYRTHIKKFLNSPDDVVEEMLDGATKAHRSYLVPINGSKRALVA
RNGPRAGKVGLVIGGGSGHEPCFLGYVGKGLADAVAIGNVFSSPPPAPILECARAASGGEGVLFVYGNYVGDVMNFEMAA
EMAQEAAIPTRTVLTTDDISSSPIEDRDGRRGVAGNFFIFKVAGAACDRGMSLKACEAVTRKANRRTYTMGVALEPCSLP
QTRRHNFEIGAEEMEIGMGIHGERGVIREKLLPADAIVDHIMDRIFSEMNASPGDRVAVLVNSFGSTPLMELYVLFRRVE
QRLSAKHITIEANWVGHYCTSLDMNGASISVMHLDQELSDLLSHPCDTAFLRVN

Sequences:

>Translated_694_residues
MAPQGKTTAGKIGQAEPADGIPLRYGDDPYVWACWLYYEDGRTQGEIANIMGISRATVNSYLADARNRGIVNISIEPARL
GSLAIAQELKHHFGLSDCLVVPNDDGAHPLIDRLGTAGAQAVTRLLKSGDVLAVAWGRTVLAIAENTCISNLQDVTVVQA
TGGTRASFAYTPELCAAAMARATNAHLVNITAPAIVSSADVKTAITGEPIIVEQFQVLNHANKALFGISSLRPNSTIHTS
GFFENVAIQDYLAKNAVGVVAGRFIDAYGQPVAGPLDDRTIGITLDRLRMIDMRIAVAGGFDKVPAILAALRGGYINVLI
TDAATGRGILNADGVTGIDSKLSQKLRAEPAANASSHYRTHIKKFLNSPDDVVEEMLDGATKAHRSYLVPINGSKRALVA
RNGPRAGKVGLVIGGGSGHEPCFLGYVGKGLADAVAIGNVFSSPPPAPILECARAASGGEGVLFVYGNYVGDVMNFEMAA
EMAQEAAIPTRTVLTTDDISSSPIEDRDGRRGVAGNFFIFKVAGAACDRGMSLKACEAVTRKANRRTYTMGVALEPCSLP
QTRRHNFEIGAEEMEIGMGIHGERGVIREKLLPADAIVDHIMDRIFSEMNASPGDRVAVLVNSFGSTPLMELYVLFRRVE
QRLSAKHITIEANWVGHYCTSLDMNGASISVMHLDQELSDLLSHPCDTAFLRVN
>Mature_693_residues
APQGKTTAGKIGQAEPADGIPLRYGDDPYVWACWLYYEDGRTQGEIANIMGISRATVNSYLADARNRGIVNISIEPARLG
SLAIAQELKHHFGLSDCLVVPNDDGAHPLIDRLGTAGAQAVTRLLKSGDVLAVAWGRTVLAIAENTCISNLQDVTVVQAT
GGTRASFAYTPELCAAAMARATNAHLVNITAPAIVSSADVKTAITGEPIIVEQFQVLNHANKALFGISSLRPNSTIHTSG
FFENVAIQDYLAKNAVGVVAGRFIDAYGQPVAGPLDDRTIGITLDRLRMIDMRIAVAGGFDKVPAILAALRGGYINVLIT
DAATGRGILNADGVTGIDSKLSQKLRAEPAANASSHYRTHIKKFLNSPDDVVEEMLDGATKAHRSYLVPINGSKRALVAR
NGPRAGKVGLVIGGGSGHEPCFLGYVGKGLADAVAIGNVFSSPPPAPILECARAASGGEGVLFVYGNYVGDVMNFEMAAE
MAQEAAIPTRTVLTTDDISSSPIEDRDGRRGVAGNFFIFKVAGAACDRGMSLKACEAVTRKANRRTYTMGVALEPCSLPQ
TRRHNFEIGAEEMEIGMGIHGERGVIREKLLPADAIVDHIMDRIFSEMNASPGDRVAVLVNSFGSTPLMELYVLFRRVEQ
RLSAKHITIEANWVGHYCTSLDMNGASISVMHLDQELSDLLSHPCDTAFLRVN

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2390

COG function: function code K; Transcriptional regulator, contains sigma factor-related N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=327, Percent_Identity=35.474006116208, Blast_Score=186, Evalue=9e-47,
Organism=Escherichia coli, GI87081857, Length=354, Percent_Identity=38.7005649717514, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI87082414, Length=314, Percent_Identity=35.3503184713376, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787791, Length=304, Percent_Identity=31.9078947368421, Blast_Score=122, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17565018, Length=325, Percent_Identity=34.4615384615385, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321055, Length=298, Percent_Identity=31.5436241610738, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323570, Length=287, Percent_Identity=31.0104529616725, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 73947; Mature: 73816

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPQGKTTAGKIGQAEPADGIPLRYGDDPYVWACWLYYEDGRTQGEIANIMGISRATVNS
CCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
YLADARNRGIVNISIEPARLGSLAIAQELKHHFGLSDCLVVPNDDGAHPLIDRLGTAGAQ
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCHHH
AVTRLLKSGDVLAVAWGRTVLAIAENTCISNLQDVTVVQATGGTRASFAYTPELCAAAMA
HHHHHHHCCCEEEEECCCEEEEEECCHHHCCCCCEEEEEECCCCCEEEEECHHHHHHHHH
RATNAHLVNITAPAIVSSADVKTAITGEPIIVEQFQVLNHANKALFGISSLRPNSTIHTS
HCCCCEEEEEECCHHCCCCCCEEEECCCCCHHHHHHHHHHCCHHHEECCCCCCCCEEECC
GFFENVAIQDYLAKNAVGVVAGRFIDAYGQPVAGPLDDRTIGITLDRLRMIDMRIAVAGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHEEEEEEEEEEECC
FDKVPAILAALRGGYINVLITDAATGRGILNADGVTGIDSKLSQKLRAEPAANASSHYRT
CHHHHHHHHHHCCCEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
HIKKFLNSPDDVVEEMLDGATKAHRSYLVPINGSKRALVARNGPRAGKVGLVIGGGSGHE
HHHHHHCCHHHHHHHHHCCHHHHCCEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCC
PCFLGYVGKGLADAVAIGNVFSSPPPAPILECARAASGGEGVLFVYGNYVGDVMNFEMAA
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHH
EMAQEAAIPTRTVLTTDDISSSPIEDRDGRRGVAGNFFIFKVAGAACDRGMSLKACEAVT
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECHHHCCCCCHHHHHHHH
RKANRRTYTMGVALEPCSLPQTRRHNFEIGAEEMEIGMGIHGERGVIREKLLPADAIVDH
HHCCCEEEEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHH
IMDRIFSEMNASPGDRVAVLVNSFGSTPLMELYVLFRRVEQRLSAKHITIEANWVGHYCT
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEE
SLDMNGASISVMHLDQELSDLLSHPCDTAFLRVN
EECCCCCEEEEEECCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
APQGKTTAGKIGQAEPADGIPLRYGDDPYVWACWLYYEDGRTQGEIANIMGISRATVNS
CCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
YLADARNRGIVNISIEPARLGSLAIAQELKHHFGLSDCLVVPNDDGAHPLIDRLGTAGAQ
HHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCHHH
AVTRLLKSGDVLAVAWGRTVLAIAENTCISNLQDVTVVQATGGTRASFAYTPELCAAAMA
HHHHHHHCCCEEEEECCCEEEEEECCHHHCCCCCEEEEEECCCCCEEEEECHHHHHHHHH
RATNAHLVNITAPAIVSSADVKTAITGEPIIVEQFQVLNHANKALFGISSLRPNSTIHTS
HCCCCEEEEEECCHHCCCCCCEEEECCCCCHHHHHHHHHHCCHHHEECCCCCCCCEEECC
GFFENVAIQDYLAKNAVGVVAGRFIDAYGQPVAGPLDDRTIGITLDRLRMIDMRIAVAGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHEEEEEEEEEEECC
FDKVPAILAALRGGYINVLITDAATGRGILNADGVTGIDSKLSQKLRAEPAANASSHYRT
CHHHHHHHHHHCCCEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
HIKKFLNSPDDVVEEMLDGATKAHRSYLVPINGSKRALVARNGPRAGKVGLVIGGGSGHE
HHHHHHCCHHHHHHHHHCCHHHHCCEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCC
PCFLGYVGKGLADAVAIGNVFSSPPPAPILECARAASGGEGVLFVYGNYVGDVMNFEMAA
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHH
EMAQEAAIPTRTVLTTDDISSSPIEDRDGRRGVAGNFFIFKVAGAACDRGMSLKACEAVT
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECHHHCCCCCHHHHHHHH
RKANRRTYTMGVALEPCSLPQTRRHNFEIGAEEMEIGMGIHGERGVIREKLLPADAIVDH
HHCCCEEEEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCHHHHHCCCHHHHHHH
IMDRIFSEMNASPGDRVAVLVNSFGSTPLMELYVLFRRVEQRLSAKHITIEANWVGHYCT
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEE
SLDMNGASISVMHLDQELSDLLSHPCDTAFLRVN
EECCCCCEEEEEECCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]