The gene/protein map for NC_012441 is currently unavailable.
Definition Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequence.
Accession NC_012441
Length 2,125,701

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The map label for this gene is hdhA [H]

Identifier: 225853088

GI number: 225853088

Start: 1585220

End: 1586014

Strand: Direct

Name: hdhA [H]

Synonym: BMEA_A1671

Alternate gene names: 225853088

Gene position: 1585220-1586014 (Clockwise)

Preceding gene: 225853087

Following gene: 225853089

Centisome position: 74.57

GC content: 57.11

Gene sequence:

>795_bases
ATGGCCTATGATGCATTTGCCGAATTTCGCATGACGGGCCACAATGTCATCATCACCGGCGGCGCACAGAATATAGGCGC
AGGCATTGCGAAAACCCTGTCAGGCGCAGGCGCAAAGGTGATGATCGCCGACCTCAACGGCGACAAGGCCAGGCAAACGG
CGGCAGCAATCCAGAAAGAAACCGGCAATGAATGCCATGGCATGAAGTGCGACGTCACTTCACTTAGCGATATCGAACAG
GTGGTGCGCGAAACCGTCTCGGCTTTTGGCGGCATTTCAACCCTCGTCAACAATGTCGGCTGGGGCGGGCGCCATGAAGA
CCCGACAGCGATCACCGAGGAAGATTTCATCGCAGCCTATAAGCTGAATACGATCAGCGCCTACCGCATGAGCATGGCTT
GCCTGCCGCATCTGCTTAAATCGCAAAACGCGACGATCACCAATTCCGGCTCTTTCTCCTCTGCTGTGCCAGCCTATGAC
ATTCTGGCCTATGGAACGGCGAAAGCTGCCCTCAACCAGATGATGGTTTCGCTGGCTCATATGCTGGCAAAGAAGGTGCG
CATCAATTCCGTGCTGATCGGCACGGTGATGACAGAAGGTTACGGTTCAGCCGGCATCGACGAGGAAATGCAGGAAAAGC
TGCGCCATCCCGATAACCTTACCGGGCGTGCCGGACGACCCGAAGATATCGCCAATACCATGTTGTGGCTGTGCTCGCCC
GCCAGCGGATGGGTCAGCGGCCAGACTATCAATGTGCATGGCGGCGGCGGCGTCACGCGCCTCTTCGGCAGGTGA

Upstream 100 bases:

>100_bases
CGGTTTCCGGCGGCGGCGTGCAGGAACTCGACTGACATAAGCCTTTTACGAAAGCAGCCGATACCAACAGGCATTCAATC
CAATTTCAGGAGAAACAGCC

Downstream 100 bases:

>100_bases
ACAAGCTTGGTGAAGCGTATCAGGAACCGGAAACAGGCGCTGCTGATTGCGGCATTGCCGCTTCTTGTCTTTTGTGGCCC
GCCACAAGCCATGGCGCAGG

Product: hypothetical protein

Products: NA

Alternate protein names: 7-alpha-HSDH [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAYDAFAEFRMTGHNVIITGGAQNIGAGIAKTLSGAGAKVMIADLNGDKARQTAAAIQKETGNECHGMKCDVTSLSDIEQ
VVRETVSAFGGISTLVNNVGWGGRHEDPTAITEEDFIAAYKLNTISAYRMSMACLPHLLKSQNATITNSGSFSSAVPAYD
ILAYGTAKAALNQMMVSLAHMLAKKVRINSVLIGTVMTEGYGSAGIDEEMQEKLRHPDNLTGRAGRPEDIANTMLWLCSP
ASGWVSGQTINVHGGGGVTRLFGR

Sequences:

>Translated_264_residues
MAYDAFAEFRMTGHNVIITGGAQNIGAGIAKTLSGAGAKVMIADLNGDKARQTAAAIQKETGNECHGMKCDVTSLSDIEQ
VVRETVSAFGGISTLVNNVGWGGRHEDPTAITEEDFIAAYKLNTISAYRMSMACLPHLLKSQNATITNSGSFSSAVPAYD
ILAYGTAKAALNQMMVSLAHMLAKKVRINSVLIGTVMTEGYGSAGIDEEMQEKLRHPDNLTGRAGRPEDIANTMLWLCSP
ASGWVSGQTINVHGGGGVTRLFGR
>Mature_263_residues
AYDAFAEFRMTGHNVIITGGAQNIGAGIAKTLSGAGAKVMIADLNGDKARQTAAAIQKETGNECHGMKCDVTSLSDIEQV
VRETVSAFGGISTLVNNVGWGGRHEDPTAITEEDFIAAYKLNTISAYRMSMACLPHLLKSQNATITNSGSFSSAVPAYDI
LAYGTAKAALNQMMVSLAHMLAKKVRINSVLIGTVMTEGYGSAGIDEEMQEKLRHPDNLTGRAGRPEDIANTMLWLCSPA
SGWVSGQTINVHGGGGVTRLFGR

Specific function: 7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=28.0487804878049, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI5031737, Length=239, Percent_Identity=28.0334728033473, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=29.3650793650794, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503301, Length=241, Percent_Identity=26.9709543568465, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4504505, Length=237, Percent_Identity=26.1603375527426, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=34.5381526104418, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=27.2, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=28.2786885245902, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=26.4, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787545, Length=256, Percent_Identity=26.953125, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=26.5625, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=25.6, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367365, Length=248, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=30.2681992337165, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=32.3076923076923, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=26.4227642276423, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17562904, Length=259, Percent_Identity=30.8880308880309, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=27.6, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=30.0751879699248, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=29.9242424242424, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=30.9433962264151, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=252, Percent_Identity=27.3809523809524, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=29.6577946768061, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=28.8973384030418, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=28.4644194756554, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=31.8725099601594, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=29.6577946768061, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=261, Percent_Identity=27.2030651340996, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=261, Percent_Identity=27.9693486590038, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=271, Percent_Identity=26.9372693726937, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=26.4, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=271, Percent_Identity=27.6752767527675, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=247, Percent_Identity=25.5060728744939, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=265, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=252, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=27.6515151515151, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=265, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=27.843137254902, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=255, Percent_Identity=26.2745098039216, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI28571526, Length=246, Percent_Identity=31.3008130081301, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357041, Length=246, Percent_Identity=31.3008130081301, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI23397609, Length=247, Percent_Identity=32.3886639676113, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=28.7937743190661, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=28.1124497991968, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24643142, Length=254, Percent_Identity=27.1653543307087, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.159 [H]

Molecular weight: Translated: 27899; Mature: 27768

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MAYDAFAEFRMTGHNVIITGGAQNIGAGIAKTLSGAGAKVMIADLNGDKARQTAAAIQKE
CCCHHHHHHEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
TGNECHGMKCDVTSLSDIEQVVRETVSAFGGISTLVNNVGWGGRHEDPTAITEEDFIAAY
CCCCCCCCEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHEEEE
KLNTISAYRMSMACLPHLLKSQNATITNSGSFSSAVPAYDILAYGTAKAALNQMMVSLAH
EECHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLAKKVRINSVLIGTVMTEGYGSAGIDEEMQEKLRHPDNLTGRAGRPEDIANTMLWLCSP
HHHHHHHHHHEEEEEHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECC
ASGWVSGQTINVHGGGGVTRLFGR
CCCCCCCCEEEEECCCCHHHHCCC
>Mature Secondary Structure 
AYDAFAEFRMTGHNVIITGGAQNIGAGIAKTLSGAGAKVMIADLNGDKARQTAAAIQKE
CCHHHHHHEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
TGNECHGMKCDVTSLSDIEQVVRETVSAFGGISTLVNNVGWGGRHEDPTAITEEDFIAAY
CCCCCCCCEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHEEEE
KLNTISAYRMSMACLPHLLKSQNATITNSGSFSSAVPAYDILAYGTAKAALNQMMVSLAH
EECHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLAKKVRINSVLIGTVMTEGYGSAGIDEEMQEKLRHPDNLTGRAGRPEDIANTMLWLCSP
HHHHHHHHHHEEEEEHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECC
ASGWVSGQTINVHGGGGVTRLFGR
CCCCCCCCEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]