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The map label for this gene is trmL [H]

Identifier: 225853026

GI number: 225853026

Start: 1517398

End: 1517877

Strand: Direct

Name: trmL [H]

Synonym: BMEA_A1602

Alternate gene names: 225853026

Gene position: 1517398-1517877 (Clockwise)

Preceding gene: 225853020

Following gene: 225853027

Centisome position: 71.38

GC content: 58.54

Gene sequence:

>480_bases
ATGGAACTGCGCGTCGCCCTTTACCAGCCCGATATTCCCGGCAATACCGGAACGATCCTGCGCATGGCCGCCTGCCTTGG
CTTTGCGGTCGATCTCATCGAGCCTGCCGGTTTCGATATTTCCGACCGCGCGCTAAAGCGTGCGGGCATGGATTATCTGG
AGCAGGCCGCCCTTACACGCCATCCCGACTGGAACCGTTTCGATGCCTGGCGAAAGAATGCCGGACGCCGCCTCGTGCTT
CTCTCCACCAAGGCGGCGATCCCCTATACCGGATATCAATTTCAGGAAAATGACATTCTCCTCTTCGGGCGCGAATCCGC
AGGCGTGCCGGAAGCGATCCACGACAACGCCGACGAACGGCTGCTGATCCCCATGGTCAGGAGCGCCCGCTCGCTAAATC
TTGCGGTTTCAGTTGCCATGACAACAGGCGAAGCTCTGCGGCAGATAAGGAGCAAAATCGATTCTCACTCAGGGAAATAG

Upstream 100 bases:

>100_bases
GGAATATTTTTTGCGAGCGACAAAAGCGTAAATTGCTGGTTCGGCCCCGAACCGGGCGCTACTCTTGCAACCATGCGGTT
TTCACGATACCGGCTTTCCC

Downstream 100 bases:

>100_bases
CCCCCGCGGCTAATTCCATTTCAGACATATTTTTTTGACTATGCTGCGAAAGGGCCGGGTCGCAGTGCAATTCTTTTTTC
ATTTCCGGTCTGAAAGGCTC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MELRVALYQPDIPGNTGTILRMAACLGFAVDLIEPAGFDISDRALKRAGMDYLEQAALTRHPDWNRFDAWRKNAGRRLVL
LSTKAAIPYTGYQFQENDILLFGRESAGVPEAIHDNADERLLIPMVRSARSLNLAVSVAMTTGEALRQIRSKIDSHSGK

Sequences:

>Translated_159_residues
MELRVALYQPDIPGNTGTILRMAACLGFAVDLIEPAGFDISDRALKRAGMDYLEQAALTRHPDWNRFDAWRKNAGRRLVL
LSTKAAIPYTGYQFQENDILLFGRESAGVPEAIHDNADERLLIPMVRSARSLNLAVSVAMTTGEALRQIRSKIDSHSGK
>Mature_159_residues
MELRVALYQPDIPGNTGTILRMAACLGFAVDLIEPAGFDISDRALKRAGMDYLEQAALTRHPDWNRFDAWRKNAGRRLVL
LSTKAAIPYTGYQFQENDILLFGRESAGVPEAIHDNADERLLIPMVRSARSLNLAVSVAMTTGEALRQIRSKIDSHSGK

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=41.6107382550336, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17640; Mature: 17640

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRVALYQPDIPGNTGTILRMAACLGFAVDLIEPAGFDISDRALKRAGMDYLEQAALTR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHC
HPDWNRFDAWRKNAGRRLVLLSTKAAIPYTGYQFQENDILLFGRESAGVPEAIHDNADER
CCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCHHHHCCCCCE
LLIPMVRSARSLNLAVSVAMTTGEALRQIRSKIDSHSGK
EEHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MELRVALYQPDIPGNTGTILRMAACLGFAVDLIEPAGFDISDRALKRAGMDYLEQAALTR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHC
HPDWNRFDAWRKNAGRRLVLLSTKAAIPYTGYQFQENDILLFGRESAGVPEAIHDNADER
CCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCEECCCCEEEEECCCCCCCHHHHCCCCCE
LLIPMVRSARSLNLAVSVAMTTGEALRQIRSKIDSHSGK
EEHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA