Click here to switch to the map view.

The map label for this gene is prs

Identifier: 225853011

GI number: 225853011

Start: 1502468

End: 1503400

Strand: Direct

Name: prs

Synonym: BMEA_A1587

Alternate gene names: 225853011

Gene position: 1502468-1503400 (Clockwise)

Preceding gene: 225853010

Following gene: 225853013

Centisome position: 70.68

GC content: 59.59

Gene sequence:

>933_bases
ATGAAACTTTTCGCAGGCAACTCCAACCGGGTTCTTGCCGAATCCGTTGCTCAATATCTCAACATTCCACTCGGCAAGGC
CAGCGTCCGTCGCTTCGCTGATCAGGAAATTTTCGTGGAGATTCAGGAAAACGTGCGCGGCGAAGACGTATTCGTTCTGC
AATCGACTTCCTACCCGGCGAACGATCACCTGATGGAACTGCTCATCATGATCGATGCCTTCCGCCGCTCCTCGGCCCGT
CGCATCACCGCCGTCCTGCCCTATTTCGGCTATGCCCGTCAGGACCGCAAACCCGGCCCGCGCACGCCGATCTCGGCAAA
GCTCGTAGCCAACCTCATCACGGAAGCCGGCGCGAGCCGCGTTTTGACCCTCGATCTCCACGCTGGCCAGATTCAGGGTT
TTTTTGATATCCCGACCGACAATCTCTATGCCGTTCCGGTCATCGCCCGCGATGTGAAAGCCAATTATGCCACCGGCAAT
TGCATGGTCGTCTCGCCCGATGTCGGCGGTGTGGTCCGCGCGCGTTCACTCGCCAAGCGCATCGATGCGCAGCTTGCCAT
CGTTGACAAGCGCCGCGAACGCCCCGGTGAATCGGAAGTCATGAACGTCATCGGCGATGTTTCCGGCAAGGACTGCCTGC
TGTTCGACGATATCGTCGATTCCGGCGGCACGCTCTGCAACGCAGCCGAAGCACTGTTGAACAAGGGCGCAAACAGCGTC
ACCGCCTATATCACGCATGGCGTTCTGTCCGGCGGCGCGGTTGCCCGCATCGCCTCGTCCAAGCTGAAGGAACTGGTCAT
CACCGATTCCATCCAGCCGACCACCGCCATCAACGATGCGCCGAATATCCGCGTGCTTTCGATCTCGGACCTGATCGGCG
AAGCCATCGCCCGCACGGCAGCGGAAGAATCGGTGTCGAGCCTGTTCGACTAG

Upstream 100 bases:

>100_bases
ACGGCTCACGCGCAACAAGAGAGATTATGTCCGTTACGACGGACTTTTGCGACAGAACCAAACTGCAAACCGCTATCGCA
TCGGCGTCAGAGGCAAAAGA

Downstream 100 bases:

>100_bases
AGTCGGTTCCGGTTAAAACGGAAACGTTGAAACCGCTCTATCTCTTTGTTTTTACGCATTATCCGACGCATCGAAGCGGG
ATCAGAAATCAGTCCGGTGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKLFAGNSNRVLAESVAQYLNIPLGKASVRRFADQEIFVEIQENVRGEDVFVLQSTSYPANDHLMELLIMIDAFRRSSAR
RITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGASRVLTLDLHAGQIQGFFDIPTDNLYAVPVIARDVKANYATGN
CMVVSPDVGGVVRARSLAKRIDAQLAIVDKRRERPGESEVMNVIGDVSGKDCLLFDDIVDSGGTLCNAAEALLNKGANSV
TAYITHGVLSGGAVARIASSKLKELVITDSIQPTTAINDAPNIRVLSISDLIGEAIARTAAEESVSSLFD

Sequences:

>Translated_310_residues
MKLFAGNSNRVLAESVAQYLNIPLGKASVRRFADQEIFVEIQENVRGEDVFVLQSTSYPANDHLMELLIMIDAFRRSSAR
RITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGASRVLTLDLHAGQIQGFFDIPTDNLYAVPVIARDVKANYATGN
CMVVSPDVGGVVRARSLAKRIDAQLAIVDKRRERPGESEVMNVIGDVSGKDCLLFDDIVDSGGTLCNAAEALLNKGANSV
TAYITHGVLSGGAVARIASSKLKELVITDSIQPTTAINDAPNIRVLSISDLIGEAIARTAAEESVSSLFD
>Mature_310_residues
MKLFAGNSNRVLAESVAQYLNIPLGKASVRRFADQEIFVEIQENVRGEDVFVLQSTSYPANDHLMELLIMIDAFRRSSAR
RITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGASRVLTLDLHAGQIQGFFDIPTDNLYAVPVIARDVKANYATGN
CMVVSPDVGGVVRARSLAKRIDAQLAIVDKRRERPGESEVMNVIGDVSGKDCLLFDDIVDSGGTLCNAAEALLNKGANSV
TAYITHGVLSGGAVARIASSKLKELVITDSIQPTTAINDAPNIRVLSISDLIGEAIARTAAEESVSSLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=308, Percent_Identity=47.4025974025974, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI4506127, Length=310, Percent_Identity=46.7741935483871, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI84875539, Length=311, Percent_Identity=46.9453376205788, Blast_Score=292, Evalue=2e-79,
Organism=Homo sapiens, GI28557709, Length=310, Percent_Identity=46.1290322580645, Blast_Score=292, Evalue=2e-79,
Organism=Homo sapiens, GI4506133, Length=342, Percent_Identity=35.0877192982456, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=33.4302325581395, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310128524, Length=143, Percent_Identity=34.2657342657343, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310115209, Length=143, Percent_Identity=34.2657342657343, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310118259, Length=143, Percent_Identity=34.2657342657343, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310119946, Length=143, Percent_Identity=34.2657342657343, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=52.8846153846154, Blast_Score=337, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=45.5128205128205, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=45.5128205128205, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=45.5128205128205, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=45.4838709677419, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=337, Percent_Identity=34.7181008902077, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321776, Length=310, Percent_Identity=46.1290322580645, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6320946, Length=311, Percent_Identity=45.3376205787781, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=45.5128205128205, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=42.3469387755102, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=105, Percent_Identity=37.1428571428571, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355239, Length=310, Percent_Identity=46.4516129032258, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI45551540, Length=333, Percent_Identity=43.2432432432432, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=32.1937321937322, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=32.1937321937322, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=32.1937321937322, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=32.1937321937322, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651462, Length=370, Percent_Identity=31.6216216216216, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24651464, Length=370, Percent_Identity=31.6216216216216, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45552010, Length=370, Percent_Identity=31.6216216216216, Blast_Score=168, Evalue=5e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_BRUME (Q8YIG1)

Other databases:

- EMBL:   AE008917
- PIR:   AE3312
- RefSeq:   NP_539400.1
- ProteinModelPortal:   Q8YIG1
- SMR:   Q8YIG1
- GeneID:   1196194
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI0483
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- PhylomeDB:   Q8YIG1
- ProtClustDB:   PRK01259
- BioCyc:   BMEL224914:BMEI0483-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 33331; Mature: 33331

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFAGNSNRVLAESVAQYLNIPLGKASVRRFADQEIFVEIQENVRGEDVFVLQSTSYPA
CEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHEEHHCCCCCEEEEEECCCCCC
NDHLMELLIMIDAFRRSSARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGASR
HHHHHHHHHHHHHHHCCCCHHEEEEHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLYAVPVIARDVKANYATGNCMVVSPDVGGVVRARSLAKR
EEEEEECCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHH
IDAQLAIVDKRRERPGESEVMNVIGDVSGKDCLLFDDIVDSGGTLCNAAEALLNKGANSV
HHHHEEEEEHHHCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCCE
TAYITHGVLSGGAVARIASSKLKELVITDSIQPTTAINDAPNIRVLSISDLIGEAIARTA
EEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AEESVSSLFD
HHHHHHHHCC
>Mature Secondary Structure
MKLFAGNSNRVLAESVAQYLNIPLGKASVRRFADQEIFVEIQENVRGEDVFVLQSTSYPA
CEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHEEHHCCCCCEEEEEECCCCCC
NDHLMELLIMIDAFRRSSARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGASR
HHHHHHHHHHHHHHHCCCCHHEEEEHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLYAVPVIARDVKANYATGNCMVVSPDVGGVVRARSLAKR
EEEEEECCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHH
IDAQLAIVDKRRERPGESEVMNVIGDVSGKDCLLFDDIVDSGGTLCNAAEALLNKGANSV
HHHHEEEEEHHHCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCCE
TAYITHGVLSGGAVARIASSKLKELVITDSIQPTTAINDAPNIRVLSISDLIGEAIARTA
EEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AEESVSSLFD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688