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The map label for this gene is pepP [C]

Identifier: 225853009

GI number: 225853009

Start: 1500370

End: 1501521

Strand: Direct

Name: pepP [C]

Synonym: BMEA_A1585

Alternate gene names: 225853009

Gene position: 1500370-1501521 (Clockwise)

Preceding gene: 225853008

Following gene: 225853010

Centisome position: 70.58

GC content: 57.81

Gene sequence:

>1152_bases
ATGGCCCTTCACTTCGAGCCGGAAGAATTCGCCGCACGTCGCGACCGTCTGATCTTGAAGATGGAAGAGGAAAAGCTGGA
TGCGCTGCTCCTGTTCGCACAGGAAAGCATGTACTGGCTGACCGGCTATGACACGTTCGGTTTCTGCTTCTTCCAGTGCC
TCGTGGTAAAGGCCGACGGTTCGATGGTGCTGATGACGCGCTCGGCCGATCTGCGGCAGGCGCGCCATACGTCAAATATC
GAAAACATCGTTGTCTGGACAGACCGCGACAATGCCAATCCGGCGGTGGATCTGCGCAATATTCTGACGGAACTCGACCT
CCTCGGCAGCCGGATCGGCATCGAATACCAGACCCATGGCCTGACCGGGGCCAATGCCCGCAAGCTTGACGAACAGTTGC
AAAGCTTTGCCAAGCTTTACGATGCATCGGGCATGGTGGATCGGCTGCGCCTTCTCAAAAGCCCTGCTGAAATCGCCTAT
GCCAAACGCGCCGCAGAACTCAGCGACGATGCGCTCGATGCCGCGGTGAAACTTACCAGGGCAGGCGCCAGCGAAGCAGA
TATTCTGGCCGCCATGATGAGCGCCAATTTTGCAGGCGACGGCGATTATCCCGCAAACGAATATATCATCGGCTCGGGCG
AAGACGCTCTCCTCTGCCGCTACAAGTCCGGTCGGCGCAAGCTGAACAAGAACGACCAGCTCACGCTGGAATGGTCTGGC
GTCTTCCGCCATTACCACGCACCGATGATGTGCACGCTGATCATCGGCAAGCCCACAAAACACCATATGGAGCTTTACGA
GGCCGCACGCGAAGCCCTCGAAGCAGTTGAAGCGGCGATGACCCCCGCCTCGACCTTCGGCGACGTGTTCGACGCCCATG
CACGGACGATGGAAGCGCACGGCCTCACCCGCCACCGGCTCAATGCCTGTGGCTATTCGGTGGGTGCCCGTTTCACGCCG
TCATGGATGGACCCGCAGATGTTTTACGCCGCAAACCCGGAGAGCATCCAGCCGGATATGACGCTTTTCGCGCATATGAT
CATCATGGATTCCGATACGCAGACCGCGATGACACTTGGCCAGACATATCTCACGACAAATGGCGCGGCTCAGCCATTAT
CCCGTCATTCTCTTGATATGATTGTCAAATGA

Upstream 100 bases:

>100_bases
TAAAACGGAATCGTTGGAACCGCTCTGATTATTTGTTTTATCGCATTTTCCAACACAAAGCCGTTTCACGCTTTTGCTGG
AAATGCCCTGGGAGAACCGC

Downstream 100 bases:

>100_bases
GGCGCAATCAGAGATAAAACCGTCAGGCGTGGACAAACTGCGCTTTCAGGAGCATGAAGCCGAACATGAACAAGGCACAT
CTTTCAATGGTGTTGTTGCT

Product: Xaa-Pro dipeptidase

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MALHFEPEEFAARRDRLILKMEEEKLDALLLFAQESMYWLTGYDTFGFCFFQCLVVKADGSMVLMTRSADLRQARHTSNI
ENIVVWTDRDNANPAVDLRNILTELDLLGSRIGIEYQTHGLTGANARKLDEQLQSFAKLYDASGMVDRLRLLKSPAEIAY
AKRAAELSDDALDAAVKLTRAGASEADILAAMMSANFAGDGDYPANEYIIGSGEDALLCRYKSGRRKLNKNDQLTLEWSG
VFRHYHAPMMCTLIIGKPTKHHMELYEAAREALEAVEAAMTPASTFGDVFDAHARTMEAHGLTRHRLNACGYSVGARFTP
SWMDPQMFYAANPESIQPDMTLFAHMIIMDSDTQTAMTLGQTYLTTNGAAQPLSRHSLDMIVK

Sequences:

>Translated_383_residues
MALHFEPEEFAARRDRLILKMEEEKLDALLLFAQESMYWLTGYDTFGFCFFQCLVVKADGSMVLMTRSADLRQARHTSNI
ENIVVWTDRDNANPAVDLRNILTELDLLGSRIGIEYQTHGLTGANARKLDEQLQSFAKLYDASGMVDRLRLLKSPAEIAY
AKRAAELSDDALDAAVKLTRAGASEADILAAMMSANFAGDGDYPANEYIIGSGEDALLCRYKSGRRKLNKNDQLTLEWSG
VFRHYHAPMMCTLIIGKPTKHHMELYEAAREALEAVEAAMTPASTFGDVFDAHARTMEAHGLTRHRLNACGYSVGARFTP
SWMDPQMFYAANPESIQPDMTLFAHMIIMDSDTQTAMTLGQTYLTTNGAAQPLSRHSLDMIVK
>Mature_382_residues
ALHFEPEEFAARRDRLILKMEEEKLDALLLFAQESMYWLTGYDTFGFCFFQCLVVKADGSMVLMTRSADLRQARHTSNIE
NIVVWTDRDNANPAVDLRNILTELDLLGSRIGIEYQTHGLTGANARKLDEQLQSFAKLYDASGMVDRLRLLKSPAEIAYA
KRAAELSDDALDAAVKLTRAGASEADILAAMMSANFAGDGDYPANEYIIGSGEDALLCRYKSGRRKLNKNDQLTLEWSGV
FRHYHAPMMCTLIIGKPTKHHMELYEAAREALEAVEAAMTPASTFGDVFDAHARTMEAHGLTRHRLNACGYSVGARFTPS
WMDPQMFYAANPESIQPDMTLFAHMIIMDSDTQTAMTLGQTYLTTNGAAQPLSRHSLDMIVK

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 42729; Mature: 42598

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHFEPEEFAARRDRLILKMEEEKLDALLLFAQESMYWLTGYDTFGFCFFQCLVVKADG
CCCCCCHHHHHHHHCCEEEEECHHHHCEEEEEECCCEEEEECCCHHHHHHHHEEEEECCC
SMVLMTRSADLRQARHTSNIENIVVWTDRDNANPAVDLRNILTELDLLGSRIGIEYQTHG
CEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECC
LTGANARKLDEQLQSFAKLYDASGMVDRLRLLKSPAEIAYAKRAAELSDDALDAAVKLTR
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AGASEADILAAMMSANFAGDGDYPANEYIIGSGEDALLCRYKSGRRKLNKNDQLTLEWSG
CCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECHHHHCCCCCCEEEEEEHH
VFRHYHAPMMCTLIIGKPTKHHMELYEAAREALEAVEAAMTPASTFGDVFDAHARTMEAH
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLTRHRLNACGYSVGARFTPSWMDPQMFYAANPESIQPDMTLFAHMIIMDSDTQTAMTLG
CCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHEEEEEECCCCHHHHHHC
QTYLTTNGAAQPLSRHSLDMIVK
CEEEECCCCCCCHHHCCHHHHCC
>Mature Secondary Structure 
ALHFEPEEFAARRDRLILKMEEEKLDALLLFAQESMYWLTGYDTFGFCFFQCLVVKADG
CCCCCHHHHHHHHCCEEEEECHHHHCEEEEEECCCEEEEECCCHHHHHHHHEEEEECCC
SMVLMTRSADLRQARHTSNIENIVVWTDRDNANPAVDLRNILTELDLLGSRIGIEYQTHG
CEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECC
LTGANARKLDEQLQSFAKLYDASGMVDRLRLLKSPAEIAYAKRAAELSDDALDAAVKLTR
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AGASEADILAAMMSANFAGDGDYPANEYIIGSGEDALLCRYKSGRRKLNKNDQLTLEWSG
CCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECHHHHCCCCCCEEEEEEHH
VFRHYHAPMMCTLIIGKPTKHHMELYEAAREALEAVEAAMTPASTFGDVFDAHARTMEAH
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLTRHRLNACGYSVGARFTPSWMDPQMFYAANPESIQPDMTLFAHMIIMDSDTQTAMTLG
CCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHEEEEEECCCCHHHHHHC
QTYLTTNGAAQPLSRHSLDMIVK
CEEEECCCCCCCHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]