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The map label for this gene is yxeI [H]

Identifier: 225852948

GI number: 225852948

Start: 1444296

End: 1445399

Strand: Reverse

Name: yxeI [H]

Synonym: BMEA_A1518

Alternate gene names: 225852948

Gene position: 1445399-1444296 (Counterclockwise)

Preceding gene: 225852950

Following gene: 225852947

Centisome position: 68.0

GC content: 54.35

Gene sequence:

>1104_bases
ATGGAAACGAAAAGCTCTCTCTGGAAATCATCGCGCCGCGTGCTTGCACATGGGGCTGCAACTGTTCTGGTCGCGGCGGG
CCTTATCGTTCCCCAGGCGGCTATGGCTTGCACGAGCTTCGTTCTGCCGACGAGCGACGGTGGTATGGTCTATGGTCGCA
CGATGGAATTCGGGTTCAATCTCAAATCCGACATGATTGCCATTCCGCGCAATTACACCATCACGGCAAGCGGGCCGGAC
GGTGCTGCGGGCAAGAAATGGAAGGGCAAATATGCCACGATCGGCATGAATGCTTTTGGTATCGTCGCTCTCACCGACGG
TATGAACGAGAAGGGGCTTGCAGGCGGGCTTCTCTATTTCCCGGAATATGCCAAGTATCAGGACCCATCCACGGCGAAGC
CGGAAGACAGCCTCGCTCCGTGGGATTTCCTGACCTGGGCGCTGGCCAATTTTTCGACAGTGGCCGAAGTCAAGGATGCT
TTGAGCACCATTTCCATCGTCGATGTGAAACAAAAGGACCTGGGATTTACCCCGCCCGCTCACTACACGCTGCATGATGC
GACCGGCGCATCCATCGTGATCGAACCGATCGACGGCAAGCTCAAGGTTTACGACAACAAGCTCGGTGTCATGACCAATT
CGCCGTCTTTCGACTGGCACATGACCAATCTGCGCAACTATGTCTATCTCTCGCGTGAAAATCCGAAGCCGTTGCAGATC
CTTGGCGAGACGATCCAGTCATTCGGGCAAGGCGCCGGTATGCATGGTATTCCGGGCGACACCACGCCGCCATCGCGTTT
CGTGCGTGCAAGCGCCTACGTCCTTTCCGCCAAGAAGGTGCCGAGCGGCCTTGAAAGCGTGCGGCTGGCCGAGCATATTG
CCAATAACTTCGACATTCCAAAGGGATGGAGCGAAGAGCAGAATATGTTTGAATATACCCAGTGGACCGCCTTTGCGGAC
ATGAAGAACGATGTCTATTACATCAAGACCTATGACGATCAGGTTCTGCGCAGCTTCAGCTTCAAGGATTTTGATGTCGA
TAGCAAAGATATTCTAACGATCAAGTTCGAGCCAAAACTGGACGCGCCGTCACTGAAAAAGTAA

Upstream 100 bases:

>100_bases
GCTGTTGACTTTAACTGATGTGCGCCATTTTATTCGTCGGAAAGTTGCCGGTCTTGTCTGGCCGGCAATGGTGGACCAAT
TGCGGATGAATGAGGTCATC

Downstream 100 bases:

>100_bases
GGCGACCATGAAAAAGGCGGCTTCGGCCGCCTTTTGACTTATCAGGGCATAATCCGGTCGGGATGACGAAGGCCGACAGG
CTTATGCCTCTACAAAAGGG

Product: penicillin acylase precursor

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPD
GAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAGGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALANFSTVAEVKDA
LSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQI
LGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVLSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFAD
MKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIKFEPKLDAPSLKK

Sequences:

>Translated_367_residues
METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPD
GAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAGGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALANFSTVAEVKDA
LSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQI
LGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVLSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFAD
MKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIKFEPKLDAPSLKK
>Mature_367_residues
METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPD
GAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAGGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALANFSTVAEVKDA
LSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQI
LGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVLSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFAD
MKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIKFEPKLDAPSLKK

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 40337; Mature: 40337

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMACTSFVLPTSDGGMVYGRTMEFGFN
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEECCC
LKSDMIAIPRNYTITASGPDGAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAGGLLYF
CCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCEEEC
PEYAKYQDPSTAKPEDSLAPWDFLTWALANFSTVAEVKDALSTISIVDVKQKDLGFTPPA
CCHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHEEEEEECHHCCCCCCCC
HYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQI
CEEEECCCCCEEEEEECCCEEEEEECEEEEEECCCCCCEEEECCCEEEEEECCCCCHHHH
LGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVLSAKKVPSGLESVRLAEHIANNFDIP
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHHCCCCC
KGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIKFEPKL
CCCCCCCCHHHHHHHEEEEECCCCEEEEEECCHHHHHHCCCCCCCCCCCCEEEEEECCCC
DAPSLKK
CCCCCCC
>Mature Secondary Structure
METKSSLWKSSRRVLAHGAATVLVAAGLIVPQAAMACTSFVLPTSDGGMVYGRTMEFGFN
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEECCC
LKSDMIAIPRNYTITASGPDGAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAGGLLYF
CCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCEEEC
PEYAKYQDPSTAKPEDSLAPWDFLTWALANFSTVAEVKDALSTISIVDVKQKDLGFTPPA
CCHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHEEEEEECHHCCCCCCCC
HYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQI
CEEEECCCCCEEEEEECCCEEEEEECEEEEEECCCCCCEEEECCCEEEEEECCCCCHHHH
LGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVLSAKKVPSGLESVRLAEHIANNFDIP
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHHCCCCC
KGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIKFEPKL
CCCCCCCCHHHHHHHEEEEECCCCEEEEEECCHHHHHHCCCCCCCCCCCCEEEEEECCCC
DAPSLKK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]