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The map label for this gene is yomI [H]

Identifier: 225852924

GI number: 225852924

Start: 1422090

End: 1422734

Strand: Reverse

Name: yomI [H]

Synonym: BMEA_A1493

Alternate gene names: 225852924

Gene position: 1422734-1422090 (Counterclockwise)

Preceding gene: 225852925

Following gene: 225852921

Centisome position: 66.93

GC content: 55.35

Gene sequence:

>645_bases
ATGAAAGCAAAATTTGTTGTGGTTTGCGCCCTCGCTGGCGCGATGGGTTCGTTTGGCCTGAATTCAGCTATGAGCGCTCC
TTCGACTGAACCGACAAACCTCGCAGCTCTTTTCAAGGCACGCCAGCAGAACAAAAATGTGGAGGTTGAGAAGCCGTCCG
TGGCTGCAAAGAAGGAAGCATCGGTTGTTACCGGACGTGCAGACCCGGTCAAGAAATCGGCAGCCCTGCGGTTGCAGCCG
AAGGACAAGCCCGCCGACAAATCTGTCAAGGATGGAAATTATTCCAGCATCATCAACCGTTATGCCGCGACCTATGGTGT
GCCTTCGCAGCTTGCCCATGCGGTTGTGCGCCACGAAAGCAATTTCAAGCCGAATGTGCGCGGCAAGGCCGGTGAAATCG
GCCTGATGCAGATCAAGCTTTCGACTGCGCGCAGCCTTGGCTATACGGGCTCGGCCAAGGGGCTTTATGAGCCTGCCACG
AATATCCAGTTCGGCATGAAATATCTCGCCATGGCGCAGAAGCTTGGCGGCGGCAGCACGTGCGGCACCATCCTGAAATA
TAATGCCGGTCACGGCGCAAAGCGCATGAACCCGACCTCCGCAAAATATTGCAATTCGGTCAAGGCCTATATGCGCTCGC
TCTAA

Upstream 100 bases:

>100_bases
TCAACGTAGGCCAGCAGGCCGGATCGGGTGGAATAAGAAAAAACGGGGCCCTCTCTCCCAAGGGCGGTCCAATAAAAGAA
ACGTTTCAAGACGGTAGGTT

Downstream 100 bases:

>100_bases
ACCCGCATTCCCTTTGCATCGGCGGCTGCGTAGAGTACAGGGTTGCCTGATGTGAACGAAAAGCCGGCAGCGGTCTTGCT
GCCGGCTTTTCTGATTTCTG

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKAKFVVVCALAGAMGSFGLNSAMSAPSTEPTNLAALFKARQQNKNVEVEKPSVAAKKEASVVTGRADPVKKSAALRLQP
KDKPADKSVKDGNYSSIINRYAATYGVPSQLAHAVVRHESNFKPNVRGKAGEIGLMQIKLSTARSLGYTGSAKGLYEPAT
NIQFGMKYLAMAQKLGGGSTCGTILKYNAGHGAKRMNPTSAKYCNSVKAYMRSL

Sequences:

>Translated_214_residues
MKAKFVVVCALAGAMGSFGLNSAMSAPSTEPTNLAALFKARQQNKNVEVEKPSVAAKKEASVVTGRADPVKKSAALRLQP
KDKPADKSVKDGNYSSIINRYAATYGVPSQLAHAVVRHESNFKPNVRGKAGEIGLMQIKLSTARSLGYTGSAKGLYEPAT
NIQFGMKYLAMAQKLGGGSTCGTILKYNAGHGAKRMNPTSAKYCNSVKAYMRSL
>Mature_214_residues
MKAKFVVVCALAGAMGSFGLNSAMSAPSTEPTNLAALFKARQQNKNVEVEKPSVAAKKEASVVTGRADPVKKSAALRLQP
KDKPADKSVKDGNYSSIINRYAATYGVPSQLAHAVVRHESNFKPNVRGKAGEIGLMQIKLSTARSLGYTGSAKGLYEPAT
NIQFGMKYLAMAQKLGGGSTCGTILKYNAGHGAKRMNPTSAKYCNSVKAYMRSL

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22693; Mature: 22693

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKFVVVCALAGAMGSFGLNSAMSAPSTEPTNLAALFKARQQNKNVEVEKPSVAAKKEA
CCCCEEEEEEHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHCCC
SVVTGRADPVKKSAALRLQPKDKPADKSVKDGNYSSIINRYAATYGVPSQLAHAVVRHES
CEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFKPNVRGKAGEIGLMQIKLSTARSLGYTGSAKGLYEPATNIQFGMKYLAMAQKLGGGST
CCCCCCCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
CGTILKYNAGHGAKRMNPTSAKYCNSVKAYMRSL
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAKFVVVCALAGAMGSFGLNSAMSAPSTEPTNLAALFKARQQNKNVEVEKPSVAAKKEA
CCCCEEEEEEHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHCCC
SVVTGRADPVKKSAALRLQPKDKPADKSVKDGNYSSIINRYAATYGVPSQLAHAVVRHES
CEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFKPNVRGKAGEIGLMQIKLSTARSLGYTGSAKGLYEPATNIQFGMKYLAMAQKLGGGST
CCCCCCCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
CGTILKYNAGHGAKRMNPTSAKYCNSVKAYMRSL
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]