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The map label for this gene is ilvB [H]

Identifier: 225852872

GI number: 225852872

Start: 1363279

End: 1365102

Strand: Reverse

Name: ilvB [H]

Synonym: BMEA_A1436

Alternate gene names: 225852872

Gene position: 1365102-1363279 (Counterclockwise)

Preceding gene: 225852873

Following gene: 225852871

Centisome position: 64.22

GC content: 58.55

Gene sequence:

>1824_bases
ATGAATGCAGGTAAACATGAGGCGGAAGCAAACGCCGGGCAAGCGCAGGACGCAGGCCATCCACGCGAAATGACCGGCGC
AGAAATGGTTATCCAGGCGCTGATCGATCAGGGTGTGGAAAGCATTTTCGGCTATCCCGGCGGCGCGGTGCTTCCGATTT
ATGACGGGCTGTTTCAGCAGGATCAAATCCAGCACGTTCTGGTTCGCCATGAGCAGGGTGCAGGCCATGCAGCGGAAGGC
TATGCGCGCTCGACGGGTAAAGTCGGCGTCATGCTCGTCACTTCGGGGCCGGGTGCAACCAATGCCGTGACGCCGTTGCA
GGATGCCTTGATGGATTCCATCCCGCTGGTCTGCATTTCCGGTCAGGTTCCCACGACGCTGATCGGTTCCGATGCGTTTC
AGGAATGCGATACGATTGGCATCACGCGCCCCTGCACCAAGCACAACTGGCTGGTAAAGGACGTCAACGACCTGTCGCGC
GTCCTGCATGAAGCTTTCCATGTCGCATCCACCGGCCGTCCCGGCCCCGTCGTCGTCGATATTCCGAAGGATATTCAGTT
CGCCAAGGGCATCTATACGCCGCCGGAAACTGCACCGCGCACAAGTTACCGTCCAGTTCTCGACGGCAATGCCGAGGCTA
TTTCCGAGGCTGTCCGTCTGCTCGCCAATGCCAGAAAGCCGGTCATTTATTCGGGCGGCGGCGTGATCAATTCCGGCCCC
GCGGCTTCCCGTCTGCTGCGTGAGCTGGTCGAGATCAGCAATTTCCCGATCACCTCCACGCTGATGGGGCTTGGCGCCTA
TCCCGCCTCCGGCAAGAACTGGCTCGGTATGCTGGGCATGCACGGCACCTATGAAGCCAACATGACCATGCATGATTGCG
ATGTCATGCTGTGCGTCGGTGCGCGTTTCGACGACCGCATCACCGGCCGCCTCAACGCTTTCTCGCCGAATTCCAAGAAA
ATTCATATCGATATCGATCCGTCGTCGATCAACAAGAACGTTCGGGTCGATGTGCCGATCATCGGCGATGTCGCGCATGT
GCTGGAGGATATCGTCCGCCAGTTTCGTGCTTTCGACCGCAAGCCGGACAGGCAGGCATTGACCGAATGGTGGGGGCAGA
TCGACCGTTGGCGCGCGCGCAATTCGCTGGCCTATACGCCGAACAAAGACGTCATCATGCCGCAATATGCCATCGAGCGG
CTTTATGAACTGACCAGGAACCGCAACACCTATATCACCACCGAGGTGGGCCAGCACCAGATGTGGGCGGCGCAATTCTT
CGGCTTTGAACAGCCGAACCGTTGGCTGACTTCCGGCGGCCTTGGCACCATGGGCTATGGTCTGCCTGCGGCGCTGGGCG
TGCAGATTGCACATCCGGATGCGCTGGTTATCGATATTGCAGGCGATGCCTCGATCCAGATGTGTATCCAGGAAATGTCG
GCAGCAATCCAGCATAATGCGCCGATCAAGATTTTCATCCTGAACAACCAGTATATGGGCATGGTGCGCCAGTGGCAGCA
ATTGCTGCATGGCAACCGCCTGTCGCATTCCTACACGGAAGCGATGCCCGATTTCGTGAAACTTGCGGAAGCATACGGCG
CGCACGGCATTCGCTGCGAGAAGCCGGGCGATCTCGATGCCGCCATTCAGGAGATGATCGATATCGACAAGCCGGTCATT
TTCGATTGCCGCGTGGCAAACCTTGCCAACTGCTTCCCGATGATCCCGTCGGGCAAGGCGCATAATGAAATGCTGTTGCC
GGACGAAGCGACAGATGAGGCCGTCGCCAATGCCATTGATGCCAAGGGCCGCGCGCTCGTCTGA

Upstream 100 bases:

>100_bases
ACCACCCGGCGAAAGCTCCCATGCGCGAAAGACAGGCTCCCTCGGGGGCCTTTTTTATTATCCAAATCAGAAATGAAATG
CAGACGACGGGAAGAGAACG

Downstream 100 bases:

>100_bases
TCCGAAGGAATTGAAATCATGAACGCACAAAACCTAGCTTCCGGATCGGCCTATTTCATTGCTGCCGAAACCCAGACGCC
CGAAACCCACACGCTTGCAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MNAGKHEAEANAGQAQDAGHPREMTGAEMVIQALIDQGVESIFGYPGGAVLPIYDGLFQQDQIQHVLVRHEQGAGHAAEG
YARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSR
VLHEAFHVASTGRPGPVVVDIPKDIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGP
AASRLLRELVEISNFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKK
IHIDIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIER
LYELTRNRNTYITTEVGQHQMWAAQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMS
AAIQHNAPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVI
FDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV

Sequences:

>Translated_607_residues
MNAGKHEAEANAGQAQDAGHPREMTGAEMVIQALIDQGVESIFGYPGGAVLPIYDGLFQQDQIQHVLVRHEQGAGHAAEG
YARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSR
VLHEAFHVASTGRPGPVVVDIPKDIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGP
AASRLLRELVEISNFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKK
IHIDIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIER
LYELTRNRNTYITTEVGQHQMWAAQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMS
AAIQHNAPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVI
FDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
>Mature_607_residues
MNAGKHEAEANAGQAQDAGHPREMTGAEMVIQALIDQGVESIFGYPGGAVLPIYDGLFQQDQIQHVLVRHEQGAGHAAEG
YARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSR
VLHEAFHVASTGRPGPVVVDIPKDIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGP
AASRLLRELVEISNFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKK
IHIDIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIER
LYELTRNRNTYITTEVGQHQMWAAQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMS
AAIQHNAPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVI
FDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=24.6017699115044, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=25.3187613843352, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=51.4084507042254, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=41.4159292035398, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=569, Percent_Identity=34.2706502636204, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=28.5714285714286, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=24.6913580246914, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=26.0945709281961, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=26.0945709281961, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=515, Percent_Identity=26.0194174757282, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=43.4188034188034, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=24.5934959349593, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=25, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66206; Mature: 66206

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGKHEAEANAGQAQDAGHPREMTGAEMVIQALIDQGVESIFGYPGGAVLPIYDGLFQQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCH
DQIQHVLVRHEQGAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCIS
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEC
GQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVD
CCCCCEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IPKDIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGP
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCH
AASRLLRELVEISNFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCEEECCCEEEEEC
ARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDR
CCCCCCCCCEEECCCCCCCEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
KPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQ
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCHH
MWAAQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMS
HHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHH
AAIQHNAPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCE
HHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
KPGDLDAAIQEMIDIDKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAID
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHC
AKGRALV
CCCCCCC
>Mature Secondary Structure
MNAGKHEAEANAGQAQDAGHPREMTGAEMVIQALIDQGVESIFGYPGGAVLPIYDGLFQQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCH
DQIQHVLVRHEQGAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCIS
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEC
GQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVD
CCCCCEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IPKDIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGP
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCH
AASRLLRELVEISNFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVG
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCEEECCCEEEEEC
ARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDR
CCCCCCCCCEEECCCCCCCEEEEEECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
KPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQ
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCHH
MWAAQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMS
HHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHH
AAIQHNAPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCE
HHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
KPGDLDAAIQEMIDIDKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAID
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHC
AKGRALV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]