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The map label for this gene is pdxJ

Identifier: 225852868

GI number: 225852868

Start: 1359944

End: 1360684

Strand: Reverse

Name: pdxJ

Synonym: BMEA_A1432

Alternate gene names: 225852868

Gene position: 1360684-1359944 (Counterclockwise)

Preceding gene: 225852870

Following gene: 225852867

Centisome position: 64.01

GC content: 59.65

Gene sequence:

>741_bases
ATGCCTGCAAAATTATCGGTCAATCTCAATGCCATCGCCATGCTGCGCAATCGCCGCGACCTGCCGTGGCCAAGCGTTAC
CGGGCTTGGGCGGGCGGCACTTGCAGCAGGCGCTGCCGGGCTGACGGTTCATCCGCGGCCCGACCAGCGGCATATCCGCT
TTTCCGACCTCGGTGATATTCGCGCGCTGATCGACGATGAATATCCGCAGGCTGAATTCAATATAGAGGGTTTTCCCAGT
GAGGCCTTTCTCGATCTTGTGGAAAAGCATGAGCCGGAACAGGTGACGCTGGTGCCGGACGATCCGATGCAGGCGACATC
CGATCATGGCTGGGATTTCATGTCGAAGGCGGATTTTCTGGCGCCCATCGTTGCCCGGCTGAAAGGGCGCGGAATGCGGG
TTTCACTCTTCGCCGATCCCGATTCGCTCGGCTATGAACGCGCCAAGGCTATCGGTGCCGACCGTGTGGAGCTTTATACC
GGGCCTTACGGCGCGACCCATGACGACCCTGCCGCAGCCGCGCGCGAGCTGGACCGGCTGGAAAAGGCGGCAAGGGCGGC
CACTGCGCTCGGCCTTGCTGTCAATGCCGGGCACGATCTCACAGTGGACAATCTTCCCGCGCTCGTAAAACGTATCCCGC
AGCTTGCCGAAGTTTCTATCGGTCATGGATTGACAGCCGATGCATTGATGTATGGGATACCGGTAACGGTAAGCCGCTAC
ATCACAGCTTTGGCTGGATAA

Upstream 100 bases:

>100_bases
TAACGCTTTGTTTTGTCGCATTTTCTGATGCAAAACCGTTTCACACTTTTGCTGGAAATGCTCTATAAAGCGGAATAATC
ATTCGGGGAGCCATGCTTCG

Downstream 100 bases:

>100_bases
AACATCAACTGGATGCGCTATGTGCATAGCTCGCGATCAAGCCTTGCAAAAAGCGCATTTGCATTCAGGTGGGACAAGCT
CTAGGGCAGTCTGCCACTGT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRAALAAGAAGLTVHPRPDQRHIRFSDLGDIRALIDDEYPQAEFNIEGFPS
EAFLDLVEKHEPEQVTLVPDDPMQATSDHGWDFMSKADFLAPIVARLKGRGMRVSLFADPDSLGYERAKAIGADRVELYT
GPYGATHDDPAAAARELDRLEKAARAATALGLAVNAGHDLTVDNLPALVKRIPQLAEVSIGHGLTADALMYGIPVTVSRY
ITALAG

Sequences:

>Translated_246_residues
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRAALAAGAAGLTVHPRPDQRHIRFSDLGDIRALIDDEYPQAEFNIEGFPS
EAFLDLVEKHEPEQVTLVPDDPMQATSDHGWDFMSKADFLAPIVARLKGRGMRVSLFADPDSLGYERAKAIGADRVELYT
GPYGATHDDPAAAARELDRLEKAARAATALGLAVNAGHDLTVDNLPALVKRIPQLAEVSIGHGLTADALMYGIPVTVSRY
ITALAG
>Mature_245_residues
PAKLSVNLNAIAMLRNRRDLPWPSVTGLGRAALAAGAAGLTVHPRPDQRHIRFSDLGDIRALIDDEYPQAEFNIEGFPSE
AFLDLVEKHEPEQVTLVPDDPMQATSDHGWDFMSKADFLAPIVARLKGRGMRVSLFADPDSLGYERAKAIGADRVELYTG
PYGATHDDPAAAARELDRLEKAARAATALGLAVNAGHDLTVDNLPALVKRIPQLAEVSIGHGLTADALMYGIPVTVSRYI
TALAG

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=234, Percent_Identity=36.7521367521368, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_BRUA1 (B2S6L0)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935281.1
- ProteinModelPortal:   B2S6L0
- SMR:   B2S6L0
- GeneID:   6327258
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I13120
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26466; Mature: 26335

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 76-76 ACT_SITE 198-198 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 106-106 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLSVNLNAIAMLRNRRDLPWPSVTGLGRAALAAGAAGLTVHPRPDQRHIRFSDLGDI
CCCEEECCHHHHEEECCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCEECCCCHHH
RALIDDEYPQAEFNIEGFPSEAFLDLVEKHEPEQVTLVPDDPMQATSDHGWDFMSKADFL
HHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
APIVARLKGRGMRVSLFADPDSLGYERAKAIGADRVELYTGPYGATHDDPAAAARELDRL
HHHHHHHCCCCEEEEEEECCCHHCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
EKAARAATALGLAVNAGHDLTVDNLPALVKRIPQLAEVSIGHGLTADALMYGIPVTVSRY
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHCCCHHHHHH
ITALAG
HHHHCC
>Mature Secondary Structure 
PAKLSVNLNAIAMLRNRRDLPWPSVTGLGRAALAAGAAGLTVHPRPDQRHIRFSDLGDI
CCEEECCHHHHEEECCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCEECCCCHHH
RALIDDEYPQAEFNIEGFPSEAFLDLVEKHEPEQVTLVPDDPMQATSDHGWDFMSKADFL
HHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
APIVARLKGRGMRVSLFADPDSLGYERAKAIGADRVELYTGPYGATHDDPAAAARELDRL
HHHHHHHCCCCEEEEEEECCCHHCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
EKAARAATALGLAVNAGHDLTVDNLPALVKRIPQLAEVSIGHGLTADALMYGIPVTVSRY
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHCCCHHHHHH
ITALAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA