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The map label for this gene is rpoA [H]

Identifier: 225852705

GI number: 225852705

Start: 1206631

End: 1207644

Strand: Reverse

Name: rpoA [H]

Synonym: BMEA_A1253

Alternate gene names: 225852705

Gene position: 1207644-1206631 (Counterclockwise)

Preceding gene: 225852706

Following gene: 225852704

Centisome position: 56.81

GC content: 56.71

Gene sequence:

>1014_bases
ATGATCCAGAAGAACTGGCAGGAACTGATCAAGCCGAACAAGGTGGATTTCATCACCCACGGCTCCCGCACGCATGCAAC
CGTCGTCGCTGAGCCGCTGGAACGCGGCTTTGGCCTGACGCTCGGCAATGCGCTGCGTCGCGTGCTTCTGTCGTCGCTTC
GCGGCGCTGCCGTGACCGCTGTTCAGATCGACGGCGTTCTGCATGAATTCTCTTCCATTCCGGGCGTTCGCGAAGACGTC
ACGGATATCGTTCTGAACATCAAGGAAATCGCCATCCGTATGGAAGGCGAGGGCCCGAAGCGCATGGTCGTCCACAAGGA
AGGCCCCGGCGTCGTTACGGCTGGCGACATTCAGACCGTTGGCGATGTCGAGATCCTGAACCCGGAACACGTCATCTGCA
CGCTGGATGAAGGCGCTGAAATCCGCATGGAATTCACCGTCAACACCGGCAAGGGATATGTGCCTGCCGACTGCAACCGC
GCGGAAGATGCTCCGATCGGTCTGATCCCGGTCGATAGCCTTTATTCTCCGGTTCGCAAGGTGTCGTACAAGATCGAGAA
CACCCGTGAAGGCCAGGTTCTCGACTATGACAAGCTGACGCTGAATATCGAGACCAATGGTTCGGTTACGGGTGAAGACG
CTGTGGCTTATGCCGCTCGCATTCTCCAGGACCAGCTTTCGATCTTCGTCAACTTTGAAGAGCCGCAGAAGGAAGCTCCG
CAGGAACAGGTTGCGGAACTGGCCTTCAATCCGGCTCTGCTCAAGAAGGTGGACGAACTGGAGCTGTCGGTGCGTTCGGC
AAACTGCCTGAAGAACGACAACATCGTCTATATCGGCGATCTGATCCAGAAGACTGAAGCCGAAATGCTGCGGACCCCGA
ATTTCGGCCGCAAGTCGCTTAACGAGATCAAGGAAGTTCTGGCATCGATGGGTCTCCATCTCGGTATGGAAATCCCAGCC
TGGCCGCCGGAAAATATCGAAGACCTCGCCAAGCGCTACGAAGACCAATATTGA

Upstream 100 bases:

>100_bases
TGTATGAGCCTCGGGCTTTTCAAGTTCGAGGCTCCTCTGCGGTTTCTTCACTTGATCGCCACGACAGGATGGTGGCGACG
GTTGATAGAAGGATAATCAC

Downstream 100 bases:

>100_bases
GCATTTCCAGCGAAAGTGCGAAGCGGTTTCGCGCGGGATAATGCTTGAAAGCAAACTGAGCGTTTCCAGTGAAAGTGCGA
AAATGGTTTCGCGTCCGGGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDV
TDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNR
AEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAP
QEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPA
WPPENIEDLAKRYEDQY

Sequences:

>Translated_337_residues
MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDV
TDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNR
AEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAP
QEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPA
WPPENIEDLAKRYEDQY
>Mature_337_residues
MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDV
TDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNR
AEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAP
QEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPA
WPPENIEDLAKRYEDQY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=51.963746223565, Blast_Score=327, Evalue=8e-91,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 37313; Mature: 37313

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTA
CCCCCHHHHHCCCCEEEEECCCCCCHHEEECHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
VQIDGVLHEFSSIPGVREDVTDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTV
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCEEEECCCCCC
GDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNRAEDAPIGLIPVDSLYSPVRK
CCEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
VSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAP
HHEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCC
QEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSL
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHH
NEIKEVLASMGLHLGMEIPAWPPENIEDLAKRYEDQY
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTA
CCCCCHHHHHCCCCEEEEECCCCCCHHEEECHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
VQIDGVLHEFSSIPGVREDVTDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTV
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCEEEECCCCCC
GDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNRAEDAPIGLIPVDSLYSPVRK
CCEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
VSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAP
HHEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCC
QEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSL
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHH
NEIKEVLASMGLHLGMEIPAWPPENIEDLAKRYEDQY
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA