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The map label for this gene is eno [H]

Identifier: 225852632

GI number: 225852632

Start: 1128911

End: 1130188

Strand: Reverse

Name: eno [H]

Synonym: BMEA_A1177

Alternate gene names: 225852632

Gene position: 1130188-1128911 (Counterclockwise)

Preceding gene: 225852633

Following gene: 225852631

Centisome position: 53.17

GC content: 57.43

Gene sequence:

>1278_bases
ATGACTGCAATCATCGACATCGTCGGCCGCGAAATTCTCGACAGCCGCGGCAACCCGACCGTCGAAGTGGATGTCGTGCT
TGAAGATGGCTCTTTCGGACGCGCGGCTGTTCCGTCCGGCGCATCGACTGGCGCGCATGAAGCCGTTGAACTGCGTGATG
GCGGCAGCCGTTATCTTGGCAAGGGCGTGGAAAAGGCAGTTGAAGTAGTCAATGGCAAGATTTTCGACGCCATTGCAGGC
ATGGACGCGGAGAGCCAGCTTCTCATCGACCAGACGCTGATCGATCTTGATGGCTCGGCCAACAAGGGCAACCTCGGCGC
CAATGCTATTCTCGGCGTTTCCCTGGCGGTTGCCAAGGCGGCTGCGCAAGCCAGCGGCCTGCCGCTTTATCGCTATGTGG
GCGGCACCAATGCGCATGTGCTTCCCGTTCCGATGATGAACATCATCAATGGCGGCGCCCATGCCGATAATCCGATCGAT
TTTCAGGAATTCATGATCCTTCCGGTTGGCGCGACTTCCATTCGCGAAGCTGTGCGCTATGGCTCGGAAGTCTTCCACAC
ACTGAAGAAGCGCCTCAAGGATGCCGGACATAACACCAATGTCGGCGACGAAGGCGGCTTTGCGCCAAACCTCAAGAATG
CACAGGCCGCACTCGATTTCATCATGGAATCGATTGAGAAGGCTGGTTTCAAGCCGGGCGAAGATATTGCTCTTGGCCTG
GACTGCGCGGCGACCGAGTTCTTCAAGGACGGCAACTACGTCTATGAAGGCGAGCGCAAGACCCGCGATCCGAAGGCGCA
GGCCAAGTATCTCGCCAAGCTTGCCAGCGACTATCCTATCGTCACCATTGAAGACGGTATGGCTGAAGACGATTGGGAAG
GCTGGAAATATCTGACCGATCTGATCGGCAATAAGTGCCAGCTTGTCGGCGACGATCTGTTCGTGACGAATTCGGCTCGT
CTGCGTGACGGTATCCGTCTGGGCGTCGCCAACTCAATTCTCGTCAAGGTGAACCAGATCGGTTCGCTGTCGGAAACGCT
GGACGCGGTCGAAACCGCCCACAAGGCTGGTTACACCGCCGTTATGTCGCATCGCTCGGGCGAAACGGAAGATTCCACCA
TTGCCGATCTTGCCGTGGCTACCAATTGCGGCCAGATCAAAACCGGCTCGCTCGCGCGTTCGGATCGCACGGCGAAGTAC
AACCAGCTCATCCGCATTGAGGAAGAGCTGGACAAGCAGGCCCGCTATGCTGGTCGCAGTGCACTGAAGTTGCTCTAA

Upstream 100 bases:

>100_bases
GATGTTTCCATTTGGCTGTAAAATGCTCTAAGGATGTGCCCATATCGGATTTGTTAATGCCTGCCGGCGGCAGGCAATCT
ATAGGAAAGGACTGGCTCCT

Downstream 100 bases:

>100_bases
GCGGAACGGGATTTAGGCAAAAGGCGGGCCACTATGCCCGCCTTTTTTCGTTTTGTTGCTTGGTGTAGTGTGGCAAGCTT
TGTTTAAGTTATCTAGAGCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAG
MDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPID
FQEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSAR
LRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLIRIEEELDKQARYAGRSALKLL

Sequences:

>Translated_425_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAG
MDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPID
FQEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSAR
LRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLIRIEEELDKQARYAGRSALKLL
>Mature_424_residues
TAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGM
DAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDF
QEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGLD
CAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSARL
RDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYN
QLIRIEEELDKQARYAGRSALKLL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=50.8045977011494, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=49.1954022988506, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=49.1990846681922, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=49.1990846681922, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=45.7471264367816, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.1791044776119, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.1791044776119, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.1791044776119, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.0722610722611, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=52.4027459954233, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=52.4027459954233, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=45.360824742268, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=49.1990846681922, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.2678983833718, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.2678983833718, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.2678983833718, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=48.7414187643021, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.2304147465438, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.2304147465438, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.2304147465438, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.2304147465438, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.2304147465438, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.2304147465438, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45320; Mature: 45188

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
KGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKA
HHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRY
HHHHCCCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHCCEEECCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLIRIEEELDKQARYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
ALKLL
HHHCC
>Mature Secondary Structure 
TAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
KGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKA
HHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRY
HHHHCCCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHCCEEECCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLIRIEEELDKQARYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
ALKLL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA