Click here to switch to the map view.

The map label for this gene is dus [H]

Identifier: 225852620

GI number: 225852620

Start: 1116341

End: 1117342

Strand: Reverse

Name: dus [H]

Synonym: BMEA_A1164

Alternate gene names: 225852620

Gene position: 1117342-1116341 (Counterclockwise)

Preceding gene: 225852623

Following gene: 225852619

Centisome position: 52.56

GC content: 58.68

Gene sequence:

>1002_bases
TTGGTGACTTCCCTCGATCAACCTTTGAATATTCGCGGTGTGATGCTCAAGAACCGCGTTTTTCTTGCGCCGATGTCCGG
TGTTTCCGATCTCCCGTTTCGCAAGCGCGCGGCTGAGGCCGGTGCGGGTATGGTCGTGTCCGAAATGGTGGCGAGTGCGG
AGCTGTGCAACCGGCACAGGGAAAGTCTGTTGCGCCTTTCCGGCGAGGGGCTGGGAACGCATGTGGTGCAGCTTGCAGGA
CGCGAGGCGCGCTGGATGGGGGAGGCTGCAAAAATCGCCGAAGGCGAGGGTACGGATATCATTGATATCAATATGGGCTG
CCCTGCGAAAAAGGTGACGGGCGGCTATTCCGGTTCGGCGCTGATGCGCAATCTCGATCATGCCATGACACTGGTTGAGG
CCACGGTTGCGGCGGTGAAAGTACCTGTCACGCTGAAGATGCGACTTGGCTGGGATGAAAACAGCATCAATGCGCCGGAG
CTTGCCAGGCGCGCCGAAGATGCGGGTATCGCCATGGTGACGGTGCATGGCCGCACGCGCTGCCAGTTTTATGAAGGCAC
TGCAGACTGGGATGCGATCCATGCGGTGCGCGATGCGGTGAGAATCCCGCTTGTCGCCAATGGAGATGTCTCATCGCGCG
CCGATGCCGAGGAAATTCTGCGCCGCTCGGGGGCTGACGCTGTAATGGTGGGACGTGCCTCCTATGGGCAGCCATGGCTG
GCGGGAGCGATCGCGGGAAGTGATTTCGCACCGCGTGATGCAAATGGAATTCTTGATTATATTATAAGACATTACGAAGA
TATGCTCGACCATTATGGCAGCAAAATAGGCATTCGTCACGCCCGCAAGCATCTGGGCTGGTATATGGATCGCCATGCCG
GGCGCGCCTTCACGCCGGCAGACAAGGCGGAACTCATGACGTTGACGGATCGGGCTGCAGTGGTTGAAACCCTCCGGCGA
ATATTCCTGCGCGATGTCGATCCGCTGCGAAAGGTTGCCTGA

Upstream 100 bases:

>100_bases
AATTGATTTATCGTGCGTTCAGGCTGCTTTTCCTTGCGTTCGGAAAGCGGGGTGATTAAACTGTATGCGGATTTCATAAT
GCATATCAAATAGGCGCTTA

Downstream 100 bases:

>100_bases
TGCGCAATAAGACTGAAACGGGCGGCGTTCCTTCCATCGTGCTTGATGCGATCAGCCAGCCGGTCATCATGGTGAGTGCG
GATAATCACATCGTCTATGC

Product: TIM-barrel protein nifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGTDIIDINMGCPAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHARKHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA

Sequences:

>Translated_333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGTDIIDINMGCPAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHARKHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA
>Mature_333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGTDIIDINMGCPAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHARKHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=288, Percent_Identity=31.25, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239788462, Length=284, Percent_Identity=30.6338028169014, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=302, Percent_Identity=26.8211920529801, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=28.6852589641434, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=30.4721030042918, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=41.5807560137457, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=34.6153846153846, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI145693211, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=30.2083333333333, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=235, Percent_Identity=30.2127659574468, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=31.4159292035398, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=27.3026315789474, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=256, Percent_Identity=31.25, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=260, Percent_Identity=28.0769230769231, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=30.6049822064057, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=30.2904564315353, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921524, Length=318, Percent_Identity=26.1006289308176, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36298; Mature: 36298

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHR
CCCCCCCCCCEEEEEEECEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ESLLRLSGEGLGTHVVQLAGREARWMGEAAKIAEGEGTDIIDINMGCPAKKVTGGYSGSA
HHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCHHH
LMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPELARRAEDAGIAMVTVHGRTR
HHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCEE
CQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
EEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHH
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHARKHLGWYMDRHAGRAFTPA
HHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DKAELMTLTDRAAVVETLRRIFLRDVDPLRKVA
CCHHEEEHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHR
CCCCCCCCCCEEEEEEECEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ESLLRLSGEGLGTHVVQLAGREARWMGEAAKIAEGEGTDIIDINMGCPAKKVTGGYSGSA
HHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCHHH
LMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPELARRAEDAGIAMVTVHGRTR
HHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCEE
CQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
EEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHH
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHARKHLGWYMDRHAGRAFTPA
HHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DKAELMTLTDRAAVVETLRRIFLRDVDPLRKVA
CCHHEEEHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]