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The map label for this gene is ntrB [H]

Identifier: 225852619

GI number: 225852619

Start: 1115199

End: 1116341

Strand: Reverse

Name: ntrB [H]

Synonym: BMEA_A1163

Alternate gene names: 225852619

Gene position: 1116341-1115199 (Counterclockwise)

Preceding gene: 225852620

Following gene: 225852618

Centisome position: 52.52

GC content: 59.58

Gene sequence:

>1143_bases
ATGCGCAATAAGACTGAAACGGGCGGCGTTCCTTCCATCGTGCTTGATGCGATCAGCCAGCCGGTCATCATGGTGAGTGC
GGATAATCACATCGTCTATGCCAATCTGGATGCGGAGCAGTTTTTCCTGTCCGGCTCATCCATTCTGGCGCGAAACCGGC
TGGAATCCTTTCTTCCCTTCGGAAGCCCCCTGCTTGCGCTGGTCGATCAGGTGCGGGCGAGCCAGATGCCGGTCAACGAA
TATCGTGTCGATATTTCTTCTCCGCGTCTGGGCGCCGAACGGATCGTGGATATTTATGTTGCACCGGTGGCGGAAATGCC
GGGAGCGGTGGTCATTCTCTTCAAGGAAAAGAGCATGGCGGAAAAGATCGACCGCCAGATGACGCATCGCGGTGCGGCGC
GTTCAGTTACCGGCCTTGCATCCATGCTGGCGCACGAGATCAAGAACCCGCTTTCCGGTATTCGCGGTGCGGCGCAGCTT
CTGGAACAGGTGGTCAGCGATGAAGATCGCGCGCTGGCGCGGCTTATCACCGAGGAGACCGACCGTATTGTCTCGCTTGT
GGACCGGATGGAGGTTTTTTCCGACGAGCGCCCCATCGAGCGCACGGCGGTCAATATCCATGCCGTGCTGGACCATGTGA
AGGCCATTGCGCGCAATGGTTTCGCACGGAAAATCCGCTTCAGCGAGGATTACGATCCATCCCTGCCGCCGGTCTATGCC
AATCGCGACCAGCTTGTGCAGGTGTTTCTCAATCTGGTGAAAAATGCCTGCGAGGCGATGGGTGGCCGCGATGGCGGCGA
GATCGTGCTTTCGACAGCCTATCGCCCCGGCATTCGCTTGCAGGTGCCGGGCGCGCGCGACCGCGTGGCGCTGCCGCTGG
AGTTCTGCGTGCATGATAACGGGCCGGGCGTGCCGCAGGACATGCTGCCGCACCTCTTCGACCCATTCGTAACCACAAAA
ACCAACGGCTCCGGGCTGGGGCTGGCGCTGGTTGCCAAGATCATCGGCGACCATGGCGGCATCATCGAATGCGACAGCCA
TCCTGCGCGCACGACTTTCCGTATTCTCATGCCAGCCTGGAAAGGCCCCGGCGAACCGGAAATCAGGTCAAACACTATTG
GCAACGCTGGAGACAATGCATGA

Upstream 100 bases:

>100_bases
ACAAGGCGGAACTCATGACGTTGACGGATCGGGCTGCAGTGGTTGAAACCCTCCGGCGAATATTCCTGCGCGATGTCGAT
CCGCTGCGAAAGGTTGCCTG

Downstream 100 bases:

>100_bases
TTGCAGGGCGTCCGACTGGAACTGTACTGGTGGCAGACGACGATGCAGCCATTCGCACGGTGCTCAATCAGGCATTGTCC
CGTGCTGGTTATGATGTGCG

Product: nitrogen regulation protein ntrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MRNKTETGGVPSIVLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNE
YRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQL
LEQVVSDEDRALARLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYA
NRDQLVQVFLNLVKNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTK
TNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA

Sequences:

>Translated_380_residues
MRNKTETGGVPSIVLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNE
YRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQL
LEQVVSDEDRALARLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYA
NRDQLVQVFLNLVKNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTK
TNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA
>Mature_380_residues
MRNKTETGGVPSIVLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNE
YRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQL
LEQVVSDEDRALARLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYA
NRDQLVQVFLNLVKNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTK
TNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=32.3863636363636, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788549, Length=285, Percent_Identity=30.1754385964912, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=30.8035714285714, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790567, Length=130, Percent_Identity=36.1538461538462, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=27.3972602739726, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=28.3842794759825, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=28.4552845528455, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41384; Mature: 41384

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKTETGGVPSIVLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPF
CCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCC
GSPLLALVDQVRASQMPVNEYRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMA
CCHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHEECHHHCCCEEEEEECCHHHH
EKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALARLITEET
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
NRDQLVQVFLNLVKNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDN
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCEECEEEEEEECC
GPGVPQDMLPHLFDPFVTTKTNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAW
CCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECC
KGPGEPEIRSNTIGNAGDNA
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNKTETGGVPSIVLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPF
CCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCC
GSPLLALVDQVRASQMPVNEYRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMA
CCHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHEECHHHCCCEEEEEECCHHHH
EKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALARLITEET
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
NRDQLVQVFLNLVKNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDN
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCEECEEEEEEECC
GPGVPQDMLPHLFDPFVTTKTNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAW
CCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECC
KGPGEPEIRSNTIGNAGDNA
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]