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The map label for this gene is pcm [H]

Identifier: 225852454

GI number: 225852454

Start: 936745

End: 937413

Strand: Direct

Name: pcm [H]

Synonym: BMEA_A0983

Alternate gene names: 225852454

Gene position: 936745-937413 (Clockwise)

Preceding gene: 225852453

Following gene: 225852455

Centisome position: 44.07

GC content: 56.05

Gene sequence:

>669_bases
ATGGTAGCCGATTTCCAGGATCTTCGCACAAAGATGGTCGATAACCAGATCCGCACGACGGACGTGACTGATCTGGCGGT
TATCGATGCGTTTCTGACCGTTCCGCGCGAGGCATTCGTTCCGGCAAGCCGTCAGGCTCTGGCCTATATCGATGAAGATC
AGCTTCTGGAAAGCGAAGGCCCGGCCCCGCGTTATATCATGGAGCCTTCGCCCTTCGCCAAGCTTGTGCAGCTTGCCCGT
GTCAGCAAGAGCGATGTCGTTCTCGATATCGGCTGCGCATCCGGCTATTCGGCGGCTGTCCTTTCCCAATTGGCTGGATC
GGTCATCGGTTTGGAAAGCGATTCCTCGCTTGCAGCCGCCGCAACGGCGCGTCTTCAGGAATTCGGTTATGACAACGTTG
TGATCGTTTCGGGCGAGTTGAGGGCAGGCTATCCGTCCGAAGCACCCTATGATGTTATTTTCATCGAAGGTGCAGTTGAT
TTCGTCCCGGACGTTCTGTTCAATCAGCTCAAGGAAGGCGGTCGCCTGGTTGCGGTCGAAGGCCGTGGAAATGCGGGCGT
TGCGCGTCTTTACGTCAAGGAAAGCGGCGTGGCTTCCGGGCGTACCGTTTTCAATGCAGCGATCAAACCGCTCCCCGGTT
TCGAGTGTGAAGAGCAGTTCGAATTTTAA

Upstream 100 bases:

>100_bases
GTGAGGCGGGGCAGTTTTGGTCCGGCTGTAACGGTTTCGATCACAGGATGCCAGCAGGCATGAAACATCGCATTGACGCG
ATAAGGAAGGGTTTAGGGCT

Downstream 100 bases:

>100_bases
TTCGGGAATTGGTACAATTCTGCAACGTTATGAAATTTGTTTGTGAATGCGGGCTTGGCGACAATGGAAATGACCCGGTT
TACGGGTTACTAAATTTCCC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MVADFQDLRTKMVDNQIRTTDVTDLAVIDAFLTVPREAFVPASRQALAYIDEDQLLESEGPAPRYIMEPSPFAKLVQLAR
VSKSDVVLDIGCASGYSAAVLSQLAGSVIGLESDSSLAAAATARLQEFGYDNVVIVSGELRAGYPSEAPYDVIFIEGAVD
FVPDVLFNQLKEGGRLVAVEGRGNAGVARLYVKESGVASGRTVFNAAIKPLPGFECEEQFEF

Sequences:

>Translated_222_residues
MVADFQDLRTKMVDNQIRTTDVTDLAVIDAFLTVPREAFVPASRQALAYIDEDQLLESEGPAPRYIMEPSPFAKLVQLAR
VSKSDVVLDIGCASGYSAAVLSQLAGSVIGLESDSSLAAAATARLQEFGYDNVVIVSGELRAGYPSEAPYDVIFIEGAVD
FVPDVLFNQLKEGGRLVAVEGRGNAGVARLYVKESGVASGRTVFNAAIKPLPGFECEEQFEF
>Mature_222_residues
MVADFQDLRTKMVDNQIRTTDVTDLAVIDAFLTVPREAFVPASRQALAYIDEDQLLESEGPAPRYIMEPSPFAKLVQLAR
VSKSDVVLDIGCASGYSAAVLSQLAGSVIGLESDSSLAAAATARLQEFGYDNVVIVSGELRAGYPSEAPYDVIFIEGAVD
FVPDVLFNQLKEGGRLVAVEGRGNAGVARLYVKESGVASGRTVFNAAIKPLPGFECEEQFEF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=169, Percent_Identity=31.9526627218935, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23817; Mature: 23817

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADFQDLRTKMVDNQIRTTDVTDLAVIDAFLTVPREAFVPASRQALAYIDEDQLLESEG
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEECHHHHHHCCC
PAPRYIMEPSPFAKLVQLARVSKSDVVLDIGCASGYSAAVLSQLAGSVIGLESDSSLAAA
CCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHH
ATARLQEFGYDNVVIVSGELRAGYPSEAPYDVIFIEGAVDFVPDVLFNQLKEGGRLVAVE
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEE
GRGNAGVARLYVKESGVASGRTVFNAAIKPLPGFECEEQFEF
CCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure
MVADFQDLRTKMVDNQIRTTDVTDLAVIDAFLTVPREAFVPASRQALAYIDEDQLLESEG
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEECHHHHHHCCC
PAPRYIMEPSPFAKLVQLARVSKSDVVLDIGCASGYSAAVLSQLAGSVIGLESDSSLAAA
CCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHH
ATARLQEFGYDNVVIVSGELRAGYPSEAPYDVIFIEGAVDFVPDVLFNQLKEGGRLVAVE
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEE
GRGNAGVARLYVKESGVASGRTVFNAAIKPLPGFECEEQFEF
CCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA