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The map label for this gene is nifS1 [H]

Identifier: 225852441

GI number: 225852441

Start: 923983

End: 925161

Strand: Direct

Name: nifS1 [H]

Synonym: BMEA_A0968

Alternate gene names: 225852441

Gene position: 923983-925161 (Clockwise)

Preceding gene: 225852438

Following gene: 225852442

Centisome position: 43.47

GC content: 60.05

Gene sequence:

>1179_bases
GTGGTCGGGAGCGGCAAGAGGCTCTATCTGGATTATAATGCCAGTGCGCCTTTGCTTGATGAAGCGCGCGATGCGATCAT
CGCGGCATTGGGCATTACGGGCAATCCATCCTCCGTTCACCGCGAGGGGCGGGCTGCCCGTGCGCTGGTCGAATCCGCCC
GCCGCAGTGTTGCGACCCTCGTTAATGCCAGGCCGGAGCATGTCTTTTTTACGTCCGGTGCGACAGAGGCGGCCTCGACG
CTGCTGACCCCCAATTACATGATGGGGCGTTCGTCGGTGCGTCTCTCACACCTTTATGTCAGCGCCACCGAACACCCATG
CATGATCGCCGGCGGACAGTTTTCGCCCGATGACATCACCGTTCTTCCGGTTGATGCCAATGGCATCCTGCGCCTGGACA
TTTTGCGCGGCCTGCTTGCCGCCCATGACAGGAGCAAGGGTCTTCCGCTGGTTGCAGTACAGGCAGCCAATAATGAAACG
GGCGTGATCCAGCCGGTGGCGGAGATCGCAGCCATCGTAAAGGCTGCGGGCGGCATTTACATCGTCGATGCCGTGCAGGC
TGCAGGGAGAATTAAATTAGATATTACAGATAATTGCGGTGATTATCTCATAATTTCCTCACACAAGATCGGTGGCCCGA
AGGGCGTCGGCGCGGTCATTGCGATCTCCGACCTCATGATGCCGCGGGCGCTGGTGCGCGGCGGCGGGCAGGAAAAAGGC
CACCGGGCGGGAACAGAGGCGCTGCCCCTGATTGCCGGTTTTGGTGCGGCTGCCGATGTGGCAAAAGCGCGAATCGACGG
CGAAGGCTGGTCATCCCTCATGCGGGATCGTCTGGAGCGTGGCCTTGCCGAAATTGCGCCAGCGGCGATCGTTCATGGCA
GTGCTGTGCCGCGCTTGCCGAATACGACTTTCTTTTCCCTGCAAGGCCAGAAGGCTGAAACGGTGCAGATCGCTTTTGAT
CTTGCAGGTATTGCGCTTTCGGCCGGGTCGGCCTGTTCTTCGGGCAAGGTGGGGCCGAGCCATGTTCTTGCTGCCATGGG
GCAGGAAGACGGGCCGGGAGCCATCCGTGTTTCGCTTGCAACGGATGCGGGCGAGGAAACAGTGGATCGATTTCTTGAAG
CCTTGCGCAAAATCGTCGAGCGTCACCAGCGCACGAAGCACGAATTGGGCGCGGTTTGA

Upstream 100 bases:

>100_bases
TTTCAGTTTCTCAAGCGGCTTCATAGGCCGTTCTTGGTTGAAAATTCAAGAAAATTGCGACTTCAGATATCTCGAAGGCG
TTGTTAAGGAGATAGGTAAG

Downstream 100 bases:

>100_bases
GCCTCTCTGATAAATAATTCTTGAATTTTATTGGCAAATTTAAATCCGGCGCTTGAAATTCAGTTTAGAGAGGTTTTAAA
GAAGCTTCACGCATACTATA

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MVGSGKRLYLDYNASAPLLDEARDAIIAALGITGNPSSVHREGRAARALVESARRSVATLVNARPEHVFFTSGATEAAST
LLTPNYMMGRSSVRLSHLYVSATEHPCMIAGGQFSPDDITVLPVDANGILRLDILRGLLAAHDRSKGLPLVAVQAANNET
GVIQPVAEIAAIVKAAGGIYIVDAVQAAGRIKLDITDNCGDYLIISSHKIGGPKGVGAVIAISDLMMPRALVRGGGQEKG
HRAGTEALPLIAGFGAAADVAKARIDGEGWSSLMRDRLERGLAEIAPAAIVHGSAVPRLPNTTFFSLQGQKAETVQIAFD
LAGIALSAGSACSSGKVGPSHVLAAMGQEDGPGAIRVSLATDAGEETVDRFLEALRKIVERHQRTKHELGAV

Sequences:

>Translated_392_residues
MVGSGKRLYLDYNASAPLLDEARDAIIAALGITGNPSSVHREGRAARALVESARRSVATLVNARPEHVFFTSGATEAAST
LLTPNYMMGRSSVRLSHLYVSATEHPCMIAGGQFSPDDITVLPVDANGILRLDILRGLLAAHDRSKGLPLVAVQAANNET
GVIQPVAEIAAIVKAAGGIYIVDAVQAAGRIKLDITDNCGDYLIISSHKIGGPKGVGAVIAISDLMMPRALVRGGGQEKG
HRAGTEALPLIAGFGAAADVAKARIDGEGWSSLMRDRLERGLAEIAPAAIVHGSAVPRLPNTTFFSLQGQKAETVQIAFD
LAGIALSAGSACSSGKVGPSHVLAAMGQEDGPGAIRVSLATDAGEETVDRFLEALRKIVERHQRTKHELGAV
>Mature_392_residues
MVGSGKRLYLDYNASAPLLDEARDAIIAALGITGNPSSVHREGRAARALVESARRSVATLVNARPEHVFFTSGATEAAST
LLTPNYMMGRSSVRLSHLYVSATEHPCMIAGGQFSPDDITVLPVDANGILRLDILRGLLAAHDRSKGLPLVAVQAANNET
GVIQPVAEIAAIVKAAGGIYIVDAVQAAGRIKLDITDNCGDYLIISSHKIGGPKGVGAVIAISDLMMPRALVRGGGQEKG
HRAGTEALPLIAGFGAAADVAKARIDGEGWSSLMRDRLERGLAEIAPAAIVHGSAVPRLPNTTFFSLQGQKAETVQIAFD
LAGIALSAGSACSSGKVGPSHVLAAMGQEDGPGAIRVSLATDAGEETVDRFLEALRKIVERHQRTKHELGAV

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=36.2924281984334, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=29.7101449275362, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI48994898, Length=395, Percent_Identity=33.6708860759494, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787970, Length=365, Percent_Identity=26.027397260274, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=388, Percent_Identity=25.5154639175258, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=383, Percent_Identity=33.9425587467363, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17533177, Length=296, Percent_Identity=28.0405405405405, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=36.5038560411311, Blast_Score=199, Evalue=5e-52,
Organism=Drosophila melanogaster, GI20129463, Length=389, Percent_Identity=34.1902313624679, Blast_Score=181, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40774; Mature: 40774

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGSGKRLYLDYNASAPLLDEARDAIIAALGITGNPSSVHREGRAARALVESARRSVATL
CCCCCCEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VNARPEHVFFTSGATEAASTLLTPNYMMGRSSVRLSHLYVSATEHPCMIAGGQFSPDDIT
HCCCCCEEEEECCCHHHHHHHCCCCHHCCCCCEEEEEEEEEECCCCEEEECCCCCCCCEE
VLPVDANGILRLDILRGLLAAHDRSKGLPLVAVQAANNETGVIQPVAEIAAIVKAAGGIY
EEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEE
IVDAVQAAGRIKLDITDNCGDYLIISSHKIGGPKGVGAVIAISDLMMPRALVRGGGQEKG
EEEEHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
HRAGTEALPLIAGFGAAADVAKARIDGEGWSSLMRDRLERGLAEIAPAAIVHGSAVPRLP
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
NTTFFSLQGQKAETVQIAFDLAGIALSAGSACSSGKVGPSHVLAAMGQEDGPGAIRVSLA
CCEEEEEECCCCCEEEEEEEEHHHHEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEE
TDAGEETVDRFLEALRKIVERHQRTKHELGAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGSGKRLYLDYNASAPLLDEARDAIIAALGITGNPSSVHREGRAARALVESARRSVATL
CCCCCCEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VNARPEHVFFTSGATEAASTLLTPNYMMGRSSVRLSHLYVSATEHPCMIAGGQFSPDDIT
HCCCCCEEEEECCCHHHHHHHCCCCHHCCCCCEEEEEEEEEECCCCEEEECCCCCCCCEE
VLPVDANGILRLDILRGLLAAHDRSKGLPLVAVQAANNETGVIQPVAEIAAIVKAAGGIY
EEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEE
IVDAVQAAGRIKLDITDNCGDYLIISSHKIGGPKGVGAVIAISDLMMPRALVRGGGQEKG
EEEEHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
HRAGTEALPLIAGFGAAADVAKARIDGEGWSSLMRDRLERGLAEIAPAAIVHGSAVPRLP
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
NTTFFSLQGQKAETVQIAFDLAGIALSAGSACSSGKVGPSHVLAAMGQEDGPGAIRVSLA
CCEEEEEECCCCCEEEEEEEEHHHHEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEE
TDAGEETVDRFLEALRKIVERHQRTKHELGAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]