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The map label for this gene is dusA [H]

Identifier: 225852375

GI number: 225852375

Start: 858377

End: 859399

Strand: Direct

Name: dusA [H]

Synonym: BMEA_A0901

Alternate gene names: 225852375

Gene position: 858377-859399 (Clockwise)

Preceding gene: 225852374

Following gene: 225852379

Centisome position: 40.38

GC content: 59.24

Gene sequence:

>1023_bases
ATGACGGTAAGATACGGAAAAGATTCAAGCTCTGTGAAAGCCAGCAGGCCGCGTTTTGCCGTTGCGCCCATGATTGACTG
GTCGGACAGGCATTGCCGGTATTTTCACAGGCTCTTTTCAAAACGCGCTCTGCTCTATACCGAAATGGTTGTCGCCGATG
CGGCCATATTCGGTCCGCGCGACCGTTTGCTTGGCTTCGATCCTGCGGAAAATCCGGTCGCTTTGCAACTTGGTGGCTCC
GATCCGGCCAAGCTCAGGCAGGCCGCGCAGATTGGTGCGGATTTCGGCTATGACGAGATCAATCTCAATGTCGGCTGCCC
GTCGGATCGTGTCCAGTCCGGGACTTTCGGGGCGTGCCTGATGTTGACGCCCGATGTGGTGGCGCGCTGTGTTGCCGCCA
TGAAGGAGACGATTTCCATCCCGGTCACGGTCAAATGCCGTATCGGCGTGGACGATCAGGATACGGAAACGGCGCTTGAT
GCTCTTGCGGATCAGGTGTTGGAAGCCGGGGCCGATGCGCTCTGGGTTCATGCGCGCAAGGCGTGCTTGAAGGGGCTTTC
GCCCAAGGAAAACCGCGAGATCCCGCCGCTCGATTATGAGCGCGTCTATCGTCTCAAGCAGCGCCTTGGTGAAACATTCG
TCGGCATCAATGGCGGCATCGCCACGCTGGAAGAGGCGCAAGTGCACTTGCAAAAGGTGGATGCGGTTATGCTGGGCCGC
GCTGCCTATCACAATCCCGCCCTGCTGGCCGATGTGGATGCCCGCCTTTATGGCGAGGGGCAGGAACCACTAGCAATGCC
TGAAATCATCGACGCCATGTGCGATTATATCGACCGCCATATCGCGTCGGGCGGAAGGCTTTCGCATGTAAGCCGCCACA
TGGTTGGTCTTTTCACGGGCCAGCCCGGTGCGCGCCGCTGGCGGCAAATTCTCTCCACGGATGCAACGAAGCCGGGTGCT
ACCAGCGATGTGGTGCGTCAGGCTTATGCCACGGTTGTGGAAGCCTGCGCCGAAGCGGCCTAA

Upstream 100 bases:

>100_bases
ATACGCGCCAATCATCCGTGCCATCGGTACAACTTTCCTGTTGTACGCCGCATCGAAACGAATGCGAGAGGCGGCAAAAG
TTCAATTAAGCAGTTAATAA

Downstream 100 bases:

>100_bases
TTAATCAGGATCAGCTGATCGCCACGCACGGTAAAGCCGCCAAGCGTTTGCAGGAAATTCATGCCGAGCAGGCTGCCCGT
CAAGAGGCCTTCCTTCGTCA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTVRYGKDSSSVKASRPRFAVAPMIDWSDRHCRYFHRLFSKRALLYTEMVVADAAIFGPRDRLLGFDPAENPVALQLGGS
DPAKLRQAAQIGADFGYDEINLNVGCPSDRVQSGTFGACLMLTPDVVARCVAAMKETISIPVTVKCRIGVDDQDTETALD
ALADQVLEAGADALWVHARKACLKGLSPKENREIPPLDYERVYRLKQRLGETFVGINGGIATLEEAQVHLQKVDAVMLGR
AAYHNPALLADVDARLYGEGQEPLAMPEIIDAMCDYIDRHIASGGRLSHVSRHMVGLFTGQPGARRWRQILSTDATKPGA
TSDVVRQAYATVVEACAEAA

Sequences:

>Translated_340_residues
MTVRYGKDSSSVKASRPRFAVAPMIDWSDRHCRYFHRLFSKRALLYTEMVVADAAIFGPRDRLLGFDPAENPVALQLGGS
DPAKLRQAAQIGADFGYDEINLNVGCPSDRVQSGTFGACLMLTPDVVARCVAAMKETISIPVTVKCRIGVDDQDTETALD
ALADQVLEAGADALWVHARKACLKGLSPKENREIPPLDYERVYRLKQRLGETFVGINGGIATLEEAQVHLQKVDAVMLGR
AAYHNPALLADVDARLYGEGQEPLAMPEIIDAMCDYIDRHIASGGRLSHVSRHMVGLFTGQPGARRWRQILSTDATKPGA
TSDVVRQAYATVVEACAEAA
>Mature_339_residues
TVRYGKDSSSVKASRPRFAVAPMIDWSDRHCRYFHRLFSKRALLYTEMVVADAAIFGPRDRLLGFDPAENPVALQLGGSD
PAKLRQAAQIGADFGYDEINLNVGCPSDRVQSGTFGACLMLTPDVVARCVAAMKETISIPVTVKCRIGVDDQDTETALDA
LADQVLEAGADALWVHARKACLKGLSPKENREIPPLDYERVYRLKQRLGETFVGINGGIATLEEAQVHLQKVDAVMLGRA
AYHNPALLADVDARLYGEGQEPLAMPEIIDAMCDYIDRHIASGGRLSHVSRHMVGLFTGQPGARRWRQILSTDATKPGAT
SDVVRQAYATVVEACAEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=295, Percent_Identity=29.8305084745763, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI31742496, Length=304, Percent_Identity=25.6578947368421, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=55.0314465408805, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1789660, Length=268, Percent_Identity=27.6119402985075, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788462, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=260, Percent_Identity=25.3846153846154, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=29.7169811320755, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=247, Percent_Identity=29.5546558704453, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=26.3736263736264, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=26.3736263736264, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37040; Mature: 36908

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRYGKDSSSVKASRPRFAVAPMIDWSDRHCRYFHRLFSKRALLYTEMVVADAAIFGPR
CCEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DRLLGFDPAENPVALQLGGSDPAKLRQAAQIGADFGYDEINLNVGCPSDRVQSGTFGACL
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCEEE
MLTPDVVARCVAAMKETISIPVTVKCRIGVDDQDTETALDALADQVLEAGADALWVHARK
EECHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
ACLKGLSPKENREIPPLDYERVYRLKQRLGETFVGINGGIATLEEAQVHLQKVDAVMLGR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAYHNPALLADVDARLYGEGQEPLAMPEIIDAMCDYIDRHIASGGRLSHVSRHMVGLFTG
HHHCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
QPGARRWRQILSTDATKPGATSDVVRQAYATVVEACAEAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVRYGKDSSSVKASRPRFAVAPMIDWSDRHCRYFHRLFSKRALLYTEMVVADAAIFGPR
CEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DRLLGFDPAENPVALQLGGSDPAKLRQAAQIGADFGYDEINLNVGCPSDRVQSGTFGACL
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCCCCEEE
MLTPDVVARCVAAMKETISIPVTVKCRIGVDDQDTETALDALADQVLEAGADALWVHARK
EECHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
ACLKGLSPKENREIPPLDYERVYRLKQRLGETFVGINGGIATLEEAQVHLQKVDAVMLGR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAYHNPALLADVDARLYGEGQEPLAMPEIIDAMCDYIDRHIASGGRLSHVSRHMVGLFTG
HHHCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
QPGARRWRQILSTDATKPGATSDVVRQAYATVVEACAEAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]