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The map label for this gene is artJ [H]

Identifier: 225852374

GI number: 225852374

Start: 857397

End: 858185

Strand: Direct

Name: artJ [H]

Synonym: BMEA_A0900

Alternate gene names: 225852374

Gene position: 857397-858185 (Clockwise)

Preceding gene: 225852372

Following gene: 225852375

Centisome position: 40.33

GC content: 55.13

Gene sequence:

>789_bases
ATGCGCGGTTTTAAGCGTATTGCGGCATGTGCCGTGATTGCGGGTTTTGCGGCTATTGGGGCAGCAAGGGCTGAAGAGCC
CCTCAAGCTCGTCATCGGAACCGAGGGGGCATATCCGCCATTTAATTTCATCAATCCTGATGGCTCGCTATCGGGCTTCG
ATGTGGATATTGCTCGGGCGCTTTGCGATGAGATGAAGGCGGAGTGCACATTCATTACGCAGGAATGGGATGGCACCATC
CCGGCATTGCAGGCAGGCAAGTTCGACGCCTATGTGTCTTCCATGTCGATTACGGAAGAACGCAAGAAGCAGGTGGACTT
TTCGGATAAATATTACAACACGCCGCCCGGCGTGGCCGCGCCCAAGGATACCGACATCAAGGGCGTGACCAAGGAAGACC
TTGCAGGCAAGACCATCGGCGTCCAGGTTTCCACAACCCACGCGAACTATGCCGAGCAGACCTTCACCGACAGCACGATC
AAGGCTTATCCCACGGCGGAAGAATATCGCCTTGATCTTGCCAATGGTCGTCTCGATGCGGTCAATGACGACAGCGTGAC
CTTGTCGGAATGGCTGAAGACGCCGGATGGCGCCTGCTGCAAGATGGTCGGCACCTACCCGCCGGACGATGCAATCCATG
GTGTCGGCGTTGGCGTTGCCTTCAAGAAGGGCCGCCCGGAACTGGTCGAAAAGTTCAACGCTGCCATCAAGGCTATTCGC
AAGAATGGCAAGTATAAGGAAATCAACGACAAATACTTTGATTTCGACGCTTACGGCGCTGAAAACTGA

Upstream 100 bases:

>100_bases
CTATCTGTTGGCGGCCCGCGTCAAAACCAATGTGAATGGTATGCGGCTGAACCAGAAAATCAGATCAGGGAACCAGCCGG
AACAAATGAGAGGACTTGAA

Downstream 100 bases:

>100_bases
TAATTGTCTTATGGGATTGCGCCAGTCTGCTACAGGCAGACTGGCGCTTTTTTGCGTTTTTAGAATCCCGTTGTGGCTTG
ATGTATGTTAGATACGCGCC

Product: extracellular solute-binding protein family 3

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRGFKRIAACAVIAGFAAIGAARAEEPLKLVIGTEGAYPPFNFINPDGSLSGFDVDIARALCDEMKAECTFITQEWDGTI
PALQAGKFDAYVSSMSITEERKKQVDFSDKYYNTPPGVAAPKDTDIKGVTKEDLAGKTIGVQVSTTHANYAEQTFTDSTI
KAYPTAEEYRLDLANGRLDAVNDDSVTLSEWLKTPDGACCKMVGTYPPDDAIHGVGVGVAFKKGRPELVEKFNAAIKAIR
KNGKYKEINDKYFDFDAYGAEN

Sequences:

>Translated_262_residues
MRGFKRIAACAVIAGFAAIGAARAEEPLKLVIGTEGAYPPFNFINPDGSLSGFDVDIARALCDEMKAECTFITQEWDGTI
PALQAGKFDAYVSSMSITEERKKQVDFSDKYYNTPPGVAAPKDTDIKGVTKEDLAGKTIGVQVSTTHANYAEQTFTDSTI
KAYPTAEEYRLDLANGRLDAVNDDSVTLSEWLKTPDGACCKMVGTYPPDDAIHGVGVGVAFKKGRPELVEKFNAAIKAIR
KNGKYKEINDKYFDFDAYGAEN
>Mature_262_residues
MRGFKRIAACAVIAGFAAIGAARAEEPLKLVIGTEGAYPPFNFINPDGSLSGFDVDIARALCDEMKAECTFITQEWDGTI
PALQAGKFDAYVSSMSITEERKKQVDFSDKYYNTPPGVAAPKDTDIKGVTKEDLAGKTIGVQVSTTHANYAEQTFTDSTI
KAYPTAEEYRLDLANGRLDAVNDDSVTLSEWLKTPDGACCKMVGTYPPDDAIHGVGVGVAFKKGRPELVEKFNAAIKAIR
KNGKYKEINDKYFDFDAYGAEN

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=38.8888888888889, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=36.8, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=36.046511627907, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=38.3966244725738, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=33.4645669291339, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=28.6821705426357, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28456; Mature: 28456

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFKRIAACAVIAGFAAIGAARAEEPLKLVIGTEGAYPPFNFINPDGSLSGFDVDIARA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
LCDEMKAECTFITQEWDGTIPALQAGKFDAYVSSMSITEERKKQVDFSDKYYNTPPGVAA
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PKDTDIKGVTKEDLAGKTIGVQVSTTHANYAEQTFTDSTIKAYPTAEEYRLDLANGRLDA
CCCCCCCCCCHHHCCCCEEEEEEEECCCCHHHHHCCCCCEEECCCHHHHEEEECCCEEEC
VNDDSVTLSEWLKTPDGACCKMVGTYPPDDAIHGVGVGVAFKKGRPELVEKFNAAIKAIR
CCCCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
KNGKYKEINDKYFDFDAYGAEN
HCCCEEECCCCEECCCCCCCCC
>Mature Secondary Structure
MRGFKRIAACAVIAGFAAIGAARAEEPLKLVIGTEGAYPPFNFINPDGSLSGFDVDIARA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
LCDEMKAECTFITQEWDGTIPALQAGKFDAYVSSMSITEERKKQVDFSDKYYNTPPGVAA
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PKDTDIKGVTKEDLAGKTIGVQVSTTHANYAEQTFTDSTIKAYPTAEEYRLDLANGRLDA
CCCCCCCCCCHHHCCCCEEEEEEEECCCCHHHHHCCCCCEEECCCHHHHEEEECCCEEEC
VNDDSVTLSEWLKTPDGACCKMVGTYPPDDAIHGVGVGVAFKKGRPELVEKFNAAIKAIR
CCCCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
KNGKYKEINDKYFDFDAYGAEN
HCCCEEECCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]