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The map label for this gene is nuoB [H]

Identifier: 225852319

GI number: 225852319

Start: 801196

End: 801777

Strand: Direct

Name: nuoB [H]

Synonym: BMEA_A0845

Alternate gene names: 225852319

Gene position: 801196-801777 (Clockwise)

Preceding gene: 225852318

Following gene: 225852320

Centisome position: 37.69

GC content: 59.97

Gene sequence:

>582_bases
ATGGGATTGACCGGCACCAATACAACGCTCGTTGCTCCGCAGCCGAAAGGCATTCTTGACCCGCGCACGGGCAAGCCCGT
CGGATCGGACGATGCATTTTTCAATGATCTGAACGGCGAGCTTTCCGATAAGGGCTTCATCGTTACATCGGCCGATGCCC
TGATTACCTGGGCGCGCACCGGCTCGCTGATGTGGATGACCTTCGGTCTGGCATGCTGCGCGGTGGAAATGATGCACATT
TCCATGCCGCGCTACGATGCCGAACGCTTCGGCATTGCGCCGCGCGCCTCACCGCGTCAGTCCGACGTGATGATCGTTGC
CGGCACGCTGACCAACAAGATGGCGCCCGCTCTGCGCAAGGTCTACGACCAGATGCCAGAGCCGCGTTATGTGATTTCTA
TGGGCTCCTGCGCCAATGGCGGCGGCTACTACCACTATTCCTACTCGGTGGTGCGTGGCTGTGATCGCGTGGTTCCGGTC
GATATTTACGTGCCGGGCTGCCCGCCAACTGCGGAGGCGCTGCTTTACGGTATCCTGCTTCTCCAGAAGAAGATACGTCG
CACCGGCACGATCGAGCGTTGA

Upstream 100 bases:

>100_bases
CTTTCGGCCAGATCGGCTGGTTCGGCTTCCTGTCCATGATGGTGTTCCTTGGCGTTCTGACCATCGGCTTCATCTATGAA
TGGAAGAAGGGGGCGCTGGA

Downstream 100 bases:

>100_bases
TCCGGCTTTCGCCGGTTTGCGCACACTAAATTTGTTAGGGTTCTGCTGCTGCCCCGCAAGGGCCTCCCAAAACAAAGCAG
GCGGCATGAGTTCTAAAGAA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MGLTGTNTTLVAPQPKGILDPRTGKPVGSDDAFFNDLNGELSDKGFIVTSADALITWARTGSLMWMTFGLACCAVEMMHI
SMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPV
DIYVPGCPPTAEALLYGILLLQKKIRRTGTIER

Sequences:

>Translated_193_residues
MGLTGTNTTLVAPQPKGILDPRTGKPVGSDDAFFNDLNGELSDKGFIVTSADALITWARTGSLMWMTFGLACCAVEMMHI
SMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPV
DIYVPGCPPTAEALLYGILLLQKKIRRTGTIER
>Mature_192_residues
GLTGTNTTLVAPQPKGILDPRTGKPVGSDDAFFNDLNGELSDKGFIVTSADALITWARTGSLMWMTFGLACCAVEMMHIS
MPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVD
IYVPGCPPTAEALLYGILLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=152, Percent_Identity=77.6315789473684, Blast_Score=252, Evalue=1e-67,
Organism=Escherichia coli, GI1788624, Length=161, Percent_Identity=45.9627329192547, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1789074, Length=129, Percent_Identity=44.1860465116279, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=42.2764227642276, Blast_Score=103, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=173, Percent_Identity=63.5838150289017, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=78.0141843971631, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=78.0141843971631, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=78.7234042553192, Blast_Score=243, Evalue=4e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21033; Mature: 20901

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTGTNTTLVAPQPKGILDPRTGKPVGSDDAFFNDLNGELSDKGFIVTSADALITWART
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECHHEEEEECC
GSLMWMTFGLACCAVEMMHISMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGILL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIER
HHHHHHHCCCCCC
>Mature Secondary Structure 
GLTGTNTTLVAPQPKGILDPRTGKPVGSDDAFFNDLNGELSDKGFIVTSADALITWART
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECHHEEEEECC
GSLMWMTFGLACCAVEMMHISMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGILL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIER
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA