Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 225852168

GI number: 225852168

Start: 659131

End: 659607

Strand: Direct

Name: smpB

Synonym: BMEA_A0684

Alternate gene names: 225852168

Gene position: 659131-659607 (Clockwise)

Preceding gene: 225852167

Following gene: 225852172

Centisome position: 31.01

GC content: 55.77

Gene sequence:

>477_bases
ATGAACAAGCCCAAGAATTCTCCCGCGCGCAAAATGATTGCCGAAAATCGCAAGGCGCGCTTCAATTTCGAGATACTCGA
CACACTCGAAGCCGGTCTGGTACTGACGGGCACCGAGGTTAAATCCTTGCGCGCCAATCAGGCCAATATTGCCGAGAGCT
ATGCAAGCTTCGAGGATGGCGAGTTCTGGCTGATTAATTCCTATATTCCGGAATATACGCAGGGCAACCGCTTCAACCAC
GAGCCGCGGCGCCTGCGCAAATTGCTGGTGAGCAGGCGGGAAATGTCGCGCCTGTTCAATTCGGTCTCGCGCGAGGGCAT
GACGGTTGTTCCATTGAAGCTCTATTTCAACGATCGTGGCCGGGCAAAGCTCGAACTGGCGCTTGCCCGAGGCAAGAAAA
CCCACGACAAGCGCGAAACGGAAAAGAAGCGCGACTGGAACCGCGAAAAAGCGCGTCTGTTGCGCGACCGGGGATGA

Upstream 100 bases:

>100_bases
CATCAACTGACCCGGTATATTTGCCAATCCGGCGAATTTTACGCTCCCGTCGCTGCGGGAGCGTTTTCATTTCCGGCATT
CCACATTATATGGTGCCATC

Downstream 100 bases:

>100_bases
ATTGCAAAGCGCATCCCGAAAAGTGCGAAGCGGTTTTCGGATAAGATGCGCGCAAGGAAAGATCTGTATCCCGAAAAGTG
CGAAGCGGTGTTCGGGGTAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MNKPKNSPARKMIAENRKARFNFEILDTLEAGLVLTGTEVKSLRANQANIAESYASFEDGEFWLINSYIPEYTQGNRFNH
EPRRLRKLLVSRREMSRLFNSVSREGMTVVPLKLYFNDRGRAKLELALARGKKTHDKRETEKKRDWNREKARLLRDRG

Sequences:

>Translated_158_residues
MNKPKNSPARKMIAENRKARFNFEILDTLEAGLVLTGTEVKSLRANQANIAESYASFEDGEFWLINSYIPEYTQGNRFNH
EPRRLRKLLVSRREMSRLFNSVSREGMTVVPLKLYFNDRGRAKLELALARGKKTHDKRETEKKRDWNREKARLLRDRG
>Mature_158_residues
MNKPKNSPARKMIAENRKARFNFEILDTLEAGLVLTGTEVKSLRANQANIAESYASFEDGEFWLINSYIPEYTQGNRFNH
EPRRLRKLLVSRREMSRLFNSVSREGMTVVPLKLYFNDRGRAKLELALARGKKTHDKRETEKKRDWNREKARLLRDRG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=155, Percent_Identity=42.5806451612903, Blast_Score=122, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_BRUA1 (B2SAC5)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934629.1
- ProteinModelPortal:   B2SAC5
- SMR:   B2SAC5
- GeneID:   6327785
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I06240
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18571; Mature: 18571

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPKNSPARKMIAENRKARFNFEILDTLEAGLVLTGTEVKSLRANQANIAESYASFEDG
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEEEECCHHHHHHCCHHHHHHHHHCCCCC
EFWLINSYIPEYTQGNRFNHEPRRLRKLLVSRREMSRLFNSVSREGMTVVPLKLYFNDRG
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
RAKLELALARGKKTHDKRETEKKRDWNREKARLLRDRG
CEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MNKPKNSPARKMIAENRKARFNFEILDTLEAGLVLTGTEVKSLRANQANIAESYASFEDG
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEEEECCHHHHHHCCHHHHHHHHHCCCCC
EFWLINSYIPEYTQGNRFNHEPRRLRKLLVSRREMSRLFNSVSREGMTVVPLKLYFNDRG
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
RAKLELALARGKKTHDKRETEKKRDWNREKARLLRDRG
CEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA