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The map label for this gene is rfbK [H]

Identifier: 225852070

GI number: 225852070

Start: 549806

End: 551095

Strand: Direct

Name: rfbK [H]

Synonym: BMEA_A0574

Alternate gene names: 225852070

Gene position: 549806-551095 (Clockwise)

Preceding gene: 225852069

Following gene: 225852071

Centisome position: 25.86

GC content: 50.78

Gene sequence:

>1290_bases
ATGGTGTATGTTAGCGCTTTCTTACAGCATCTTTCTCATAATTTTTCATATGAAACAGTTTACGTTGGAGCCGACCTTCG
GGAGTCTAGCCCTAAAATAACCATATCATGTTATAGAGCTATTGAACTGACGGGAAGGAGAGCGATTTGGGCTGGAAATG
TTCCGACGCCGGCGCTCGCGGCTTATGCAATGGCTCCCAATGCACCTGCCATCATGATAACGGGAAGCCATATCCCAGAG
GCCTACAATGGCATTAAATTCTATCGGCCTGACGGAGAATTTCTGAAGGACGATGAAGCACCCGTACGTGATCTTGCCGA
AGAATTATTGAGTAAGGTTGTTGACGGGCAACGGTCCGTCAATTTGCCTGCGCCCTTGGCTGATGTTGCAGAAGAATATG
TGAGCCGTTCCATCGGCGCTTTTGGCCGGGACACACTGGCTGGAATGAAAATCGGAATTGATCTTCATTCCGCTGTTGGG
CGAGATATTCTTGTACGTATATTTAAAGGACTTGGAGCGGAGGTTTATCCATTCCGTAGAACGGAAAATTTCGTTGCCGT
TGATACAGAAGCGCTTGATCCAGCGGATATATCTCGGGCGCGCACATTTATCGCCGAACATGGGCTCGATGCAGTAGTGT
CGACAGATGGAGACGGTGATAGGCCTCTGGTCATTGACGATCAGGGAAGACAGGTAAATGGCGATACACTGGGTATTCTG
ACGGCTCGATATCTCCGTGCGAAAACAGTCGTGACGCCGCTCAGTACGACGAGCGCACTAGAGGAGAGTGGCTGGTTCGA
GAATATCCACAGAACACGGATTGGGTCGCCCTATGTTGTGGTCGAAATGGCGCGTGCGGTGGCGCATCCAGTTGTTGGTT
TCGAGGCGAATGGCGGCTTTCTACTCGGCGACGATGTGGCGTTGAAGACTGGTTTATTGCGCCGCCTCCCAACGCGGGAT
GCAGTTTTGCCAGCAGTGGCTGTTCTTGCGCAGGCGAAAGACCAGGGTATGCGATTGAGTGAGATGGTCGCTACACTTCC
TTCTCGTTTCATGAAAGCCGATAGGGTTAAGGAAGTTCCCGGAGACCGCGCAGCTCCATTTTTGCACGCTATAGAAACGT
CACAATCATTTCGTTCTAATTTCTCCTCGTTGATTGCCGAACCGGAAGCAATCTCGACTGTCGACGGCGTGCGAATGGCG
TTTGCGAATGGCGATACGGTTCATTTCCGACAATCAGGCAATGCTCCGGAAATGCGGATCTATAGAAACGGATTCCGCGG
AGAAAACTGA

Upstream 100 bases:

>100_bases
CTACATTTAAGTTAGATTGAGGAAAACCACAGAGATGCCTGTTGCCTTTGGAACTTCTGGCTTGAGGGGAGAGTCCGTCG
ATTTCACGGACAATGTTTGT

Downstream 100 bases:

>100_bases
TCGAATGTTGAGCGAATTTATTGCAAAGCTCTCTGAAACGATCTGAATTCATCCGCGAAAGGAAGTTAAAGTGCGTGAAA
TGTTGATGAGACCAGTAGTT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVYVSAFLQHLSHNFSYETVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPALAAYAMAPNAPAIMITGSHIPE
AYNGIKFYRPDGEFLKDDEAPVRDLAEELLSKVVDGQRSVNLPAPLADVAEEYVSRSIGAFGRDTLAGMKIGIDLHSAVG
RDILVRIFKGLGAEVYPFRRTENFVAVDTEALDPADISRARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGIL
TARYLRAKTVVTPLSTTSALEESGWFENIHRTRIGSPYVVVEMARAVAHPVVGFEANGGFLLGDDVALKTGLLRRLPTRD
AVLPAVAVLAQAKDQGMRLSEMVATLPSRFMKADRVKEVPGDRAAPFLHAIETSQSFRSNFSSLIAEPEAISTVDGVRMA
FANGDTVHFRQSGNAPEMRIYRNGFRGEN

Sequences:

>Translated_429_residues
MVYVSAFLQHLSHNFSYETVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPALAAYAMAPNAPAIMITGSHIPE
AYNGIKFYRPDGEFLKDDEAPVRDLAEELLSKVVDGQRSVNLPAPLADVAEEYVSRSIGAFGRDTLAGMKIGIDLHSAVG
RDILVRIFKGLGAEVYPFRRTENFVAVDTEALDPADISRARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGIL
TARYLRAKTVVTPLSTTSALEESGWFENIHRTRIGSPYVVVEMARAVAHPVVGFEANGGFLLGDDVALKTGLLRRLPTRD
AVLPAVAVLAQAKDQGMRLSEMVATLPSRFMKADRVKEVPGDRAAPFLHAIETSQSFRSNFSSLIAEPEAISTVDGVRMA
FANGDTVHFRQSGNAPEMRIYRNGFRGEN
>Mature_429_residues
MVYVSAFLQHLSHNFSYETVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPALAAYAMAPNAPAIMITGSHIPE
AYNGIKFYRPDGEFLKDDEAPVRDLAEELLSKVVDGQRSVNLPAPLADVAEEYVSRSIGAFGRDTLAGMKIGIDLHSAVG
RDILVRIFKGLGAEVYPFRRTENFVAVDTEALDPADISRARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGIL
TARYLRAKTVVTPLSTTSALEESGWFENIHRTRIGSPYVVVEMARAVAHPVVGFEANGGFLLGDDVALKTGLLRRLPTRD
AVLPAVAVLAQAKDQGMRLSEMVATLPSRFMKADRVKEVPGDRAAPFLHAIETSQSFRSNFSSLIAEPEAISTVDGVRMA
FANGDTVHFRQSGNAPEMRIYRNGFRGEN

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1789566, Length=390, Percent_Identity=25.6410256410256, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 46678; Mature: 46678

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYVSAFLQHLSHNFSYETVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPALA
CEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCHHHH
AYAMAPNAPAIMITGSHIPEAYNGIKFYRPDGEFLKDDEAPVRDLAEELLSKVVDGQRSV
HHHCCCCCCEEEEECCCCCHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NLPAPLADVAEEYVSRSIGAFGRDTLAGMKIGIDLHSAVGRDILVRIFKGLGAEVYPFRR
CCCCCHHHHHHHHHHHHHCCCCCHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC
TENFVAVDTEALDPADISRARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGIL
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCEECCCCHHHH
TARYLRAKTVVTPLSTTSALEESGWFENIHRTRIGSPYVVVEMARAVAHPVVGFEANGGF
HHHHHHHHHEECCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCE
LLGDDVALKTGLLRRLPTRDAVLPAVAVLAQAKDQGMRLSEMVATLPSRFMKADRVKEVP
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GDRAAPFLHAIETSQSFRSNFSSLIAEPEAISTVDGVRMAFANGDTVHFRQSGNAPEMRI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCCEEEEECCCCCCCEEE
YRNGFRGEN
EECCCCCCC
>Mature Secondary Structure
MVYVSAFLQHLSHNFSYETVYVGADLRESSPKITISCYRAIELTGRRAIWAGNVPTPALA
CEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCHHHH
AYAMAPNAPAIMITGSHIPEAYNGIKFYRPDGEFLKDDEAPVRDLAEELLSKVVDGQRSV
HHHCCCCCCEEEEECCCCCHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NLPAPLADVAEEYVSRSIGAFGRDTLAGMKIGIDLHSAVGRDILVRIFKGLGAEVYPFRR
CCCCCHHHHHHHHHHHHHCCCCCHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC
TENFVAVDTEALDPADISRARTFIAEHGLDAVVSTDGDGDRPLVIDDQGRQVNGDTLGIL
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCEECCCCHHHH
TARYLRAKTVVTPLSTTSALEESGWFENIHRTRIGSPYVVVEMARAVAHPVVGFEANGGF
HHHHHHHHHEECCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCE
LLGDDVALKTGLLRRLPTRDAVLPAVAVLAQAKDQGMRLSEMVATLPSRFMKADRVKEVP
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GDRAAPFLHAIETSQSFRSNFSSLIAEPEAISTVDGVRMAFANGDTVHFRQSGNAPEMRI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCCEEEEECCCCCCCEEE
YRNGFRGEN
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]