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The map label for this gene is ccrM

Identifier: 225852027

GI number: 225852027

Start: 510593

End: 511726

Strand: Direct

Name: ccrM

Synonym: BMEA_A0528

Alternate gene names: 225852027

Gene position: 510593-511726 (Clockwise)

Preceding gene: 225852023

Following gene: 225852030

Centisome position: 24.02

GC content: 59.88

Gene sequence:

>1134_bases
ATGTCCCTAGTACGTCTTGCGCATGAGTTGCCCATCGAGGCCCCGCGTACCGCCTGGCTCGACTCCATCATCAAAGGTGA
TTGCGTTTCCGCGCTGGAGCGCCTGTCGGATCATTCCGTAGACGTCATCTTTGCCGATCCGCCCTATAATCTCCAGCTTG
GCGGCGATCTGCACCGTCCGGATCAGTCCATGGTCAGCGCCGTGGACGATCATTGGGACCAGTTTGAAAGCTTCCAGGCC
TATGACGCCTTCACCCGCGCCTGGCTGCTCGCCTGCCGCCGTGTGCTGAAGCCGAATGGCACCATCTGGGTCATCGGTTC
CTATCACAATATTTTCCGCGTCGGCACGCAGTTGCAGGATCTGGGCTTCTGGCTCCTCAACGACATTGTCTGGCGCAAGA
CCAATCCCATGCCGAATTTCCGTGGCCGCCGTTTCCAGAATGCGCATGAAACGCTGATCTGGGCTTCGCGTGACCAGAAG
GGCAAGGGATATACTTTCAATTACGAGGCCATGAAAGCGGCCAATGACGATGTGCAGATGCGTTCGGACTGGCTGTTCCC
GATCTGCACCGGCAGTGAACGCCTGAAGGACGAGAACGGCGACAAGGTCCACCCGACCCAGAAGCCGGAAGCACTTCTCG
CGCGCATCATGATGGCTTCAAGCAAGCCGGGCGACGTTATTCTCGACCCATTCTTCGGTTCCGGCACGACCGGCGCGGTC
GCCAAGCGGCTTGGCCGCCATTTCGTCGGCATCGAGCGTGAACAGCCCTATATCGACGCCGCAACCGCCCGCATCAATGC
CGTGGAGCCGCTTGGCAAGGCGGAACTCACGGTGATGACCGGCAAGCGCGCAGAGCCGCGCGTGGCCTTCACGAGCGTAA
TGGAAGCGGGCCTTTTGCGTCCGGGAACCGTGCTTTGTGATGAACGCCGCCGTTTTGCCGCCATTGTTCGCGCCGATGGG
ACGCTGACGGCCAACGGCGAAGCCGGTTCAATCCATCGTATTGGCGCCAGGGTTCAAGGGTTCGATGCCTGCAATGGCTG
GACCTTCTGGCACTTTGAGGAAAACGGCGTACTGAAGCCTATCGATGCCCTGCGCAAGATCATCCGCGAACAGATGGCTG
CGGCAGGTGCATAA

Upstream 100 bases:

>100_bases
AAAGATTTACGATTTCAAGCACTTGGCGTTAACGGCATATTTACCCTACGCAGTAACCATAGGAACAAGTTTTTTGCGTT
CACAGGTAATCGAGTATCCC

Downstream 100 bases:

>100_bases
GAAAGTTTAATATCGGACGATCTCCAGTAAAGTCTGATAGCAAGGCGCTCGAAGTTTTCAAACTTCGGGCGCCTTCATTC
TTTCAGAAAGAAAGCTGTCG

Product: modification methylase HinfI

Products: NA

Alternate protein names: M.BabI; Adenine-specific methyltransferase BabI; DNA methyltransferase CcrM; M.CcrMI ortholog

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSLVRLAHELPIEAPRTAWLDSIIKGDCVSALERLSDHSVDVIFADPPYNLQLGGDLHRPDQSMVSAVDDHWDQFESFQA
YDAFTRAWLLACRRVLKPNGTIWVIGSYHNIFRVGTQLQDLGFWLLNDIVWRKTNPMPNFRGRRFQNAHETLIWASRDQK
GKGYTFNYEAMKAANDDVQMRSDWLFPICTGSERLKDENGDKVHPTQKPEALLARIMMASSKPGDVILDPFFGSGTTGAV
AKRLGRHFVGIEREQPYIDAATARINAVEPLGKAELTVMTGKRAEPRVAFTSVMEAGLLRPGTVLCDERRRFAAIVRADG
TLTANGEAGSIHRIGARVQGFDACNGWTFWHFEENGVLKPIDALRKIIREQMAAAGA

Sequences:

>Translated_377_residues
MSLVRLAHELPIEAPRTAWLDSIIKGDCVSALERLSDHSVDVIFADPPYNLQLGGDLHRPDQSMVSAVDDHWDQFESFQA
YDAFTRAWLLACRRVLKPNGTIWVIGSYHNIFRVGTQLQDLGFWLLNDIVWRKTNPMPNFRGRRFQNAHETLIWASRDQK
GKGYTFNYEAMKAANDDVQMRSDWLFPICTGSERLKDENGDKVHPTQKPEALLARIMMASSKPGDVILDPFFGSGTTGAV
AKRLGRHFVGIEREQPYIDAATARINAVEPLGKAELTVMTGKRAEPRVAFTSVMEAGLLRPGTVLCDERRRFAAIVRADG
TLTANGEAGSIHRIGARVQGFDACNGWTFWHFEENGVLKPIDALRKIIREQMAAAGA
>Mature_376_residues
SLVRLAHELPIEAPRTAWLDSIIKGDCVSALERLSDHSVDVIFADPPYNLQLGGDLHRPDQSMVSAVDDHWDQFESFQAY
DAFTRAWLLACRRVLKPNGTIWVIGSYHNIFRVGTQLQDLGFWLLNDIVWRKTNPMPNFRGRRFQNAHETLIWASRDQKG
KGYTFNYEAMKAANDDVQMRSDWLFPICTGSERLKDENGDKVHPTQKPEALLARIMMASSKPGDVILDPFFGSGTTGAVA
KRLGRHFVGIEREQPYIDAATARINAVEPLGKAELTVMTGKRAEPRVAFTSVMEAGLLRPGTVLCDERRRFAAIVRADGT
LTANGEAGSIHRIGARVQGFDACNGWTFWHFEENGVLKPIDALRKIIREQMAAAGA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082238, Length=287, Percent_Identity=32.0557491289199, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTB1_BRUME (Q8YFS6)

Other databases:

- EMBL:   AE008917
- PIR:   AF3432
- RefSeq:   NP_540361.1
- HSSP:   P23192
- ProteinModelPortal:   Q8YFS6
- SMR:   Q8YFS6
- REBASE:   5746
- GeneID:   1197155
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1444
- NMPDR:   fig|224914.1.peg.1443
- HOGENOM:   HBG501679
- OMA:   WRKANPM
- PhylomeDB:   Q8YFS6
- ProtClustDB:   CLSK2518600
- BioCyc:   BMEL224914:BMEI1444-MONOMER
- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091
- PRINTS:   PR00508

Pfam domain/function: PF01555 N6_N4_Mtase

EC number: =2.1.1.72

Molecular weight: Translated: 42179; Mature: 42047

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVRLAHELPIEAPRTAWLDSIIKGDCVSALERLSDHSVDVIFADPPYNLQLGGDLHRP
CCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCC
DQSMVSAVDDHWDQFESFQAYDAFTRAWLLACRRVLKPNGTIWVIGSYHNIFRVGTQLQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHH
LGFWLLNDIVWRKTNPMPNFRGRRFQNAHETLIWASRDQKGKGYTFNYEAMKAANDDVQM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHCCCCCCEE
RSDWLFPICTGSERLKDENGDKVHPTQKPEALLARIMMASSKPGDVILDPFFGSGTTGAV
CCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
AKRLGRHFVGIEREQPYIDAATARINAVEPLGKAELTVMTGKRAEPRVAFTSVMEAGLLR
HHHHHHHHCCCCCCCCCHHHHHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
PGTVLCDERRRFAAIVRADGTLTANGEAGSIHRIGARVQGFDACNGWTFWHFEENGVLKP
CCCEEECCCHHEEEEEECCCEEEECCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCH
IDALRKIIREQMAAAGA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLVRLAHELPIEAPRTAWLDSIIKGDCVSALERLSDHSVDVIFADPPYNLQLGGDLHRP
CHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCC
DQSMVSAVDDHWDQFESFQAYDAFTRAWLLACRRVLKPNGTIWVIGSYHNIFRVGTQLQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHH
LGFWLLNDIVWRKTNPMPNFRGRRFQNAHETLIWASRDQKGKGYTFNYEAMKAANDDVQM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHCCCCCCEE
RSDWLFPICTGSERLKDENGDKVHPTQKPEALLARIMMASSKPGDVILDPFFGSGTTGAV
CCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
AKRLGRHFVGIEREQPYIDAATARINAVEPLGKAELTVMTGKRAEPRVAFTSVMEAGLLR
HHHHHHHHCCCCCCCCCHHHHHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
PGTVLCDERRRFAAIVRADGTLTANGEAGSIHRIGARVQGFDACNGWTFWHFEENGVLKP
CCCEEECCCHHEEEEEECCCEEEECCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCH
IDALRKIIREQMAAAGA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688