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The map label for this gene is mscL

Identifier: 225851864

GI number: 225851864

Start: 352115

End: 352531

Strand: Direct

Name: mscL

Synonym: BMEA_A0356

Alternate gene names: 225851864

Gene position: 352115-352531 (Clockwise)

Preceding gene: 225851861

Following gene: 225851870

Centisome position: 16.56

GC content: 52.28

Gene sequence:

>417_bases
ATGTTAAAAGAATTTCAGGAATTCGCCCTGAAGGGCAATATGGTCGATCTAGCCATCGGTGTCATCATCGGCGGGGCGTT
CGGTGGCCTCGTCAACTCCATCGTCAATGATATCATCATGCCGATTATCGGCCTTATCACTGGCGGCATTGATTTCTCCA
ATATGTTCATCCAGCTTGCCGGAGACCCGAAAACCACTCTGGCCGCAGCCCGCGAAGCCGGTGCGACAATTGCCTATGGC
AATTTCATCACCCTGCTCATCAACTTCCTGATTATCGCCTGGGTGCTGTTTCTTGTGGTCAAGCTCATGAACCGCCTCAA
GAAGAGGGAAGAAGCAAAGCCTGCACCGGCAGCTCCGTCTGAGGAGGTTCTGCTTACGGAAATCCGCGATATCCTGGCAA
AGCAGCAAAAAGCCTGA

Upstream 100 bases:

>100_bases
CATTCACCGCCAGAGCCATTTGGCGCCAGTTGTTTTGACGAATGCATCGCAGCCATGACGGCCCAGCCTGCGATCCGCAT
TCGTGAGGGAGACAGGCTTT

Downstream 100 bases:

>100_bases
TGGAATTCCAGCAGGCTCTGAAACTGAAAAAGGCCGGGAAAACCGGCCTTTTTCAGTTTCATATTCGATGAGCTATCAGC
CGCCCGCCTTGGAAACCATC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MLKEFQEFALKGNMVDLAIGVIIGGAFGGLVNSIVNDIIMPIIGLITGGIDFSNMFIQLAGDPKTTLAAAREAGATIAYG
NFITLLINFLIIAWVLFLVVKLMNRLKKREEAKPAPAAPSEEVLLTEIRDILAKQQKA

Sequences:

>Translated_138_residues
MLKEFQEFALKGNMVDLAIGVIIGGAFGGLVNSIVNDIIMPIIGLITGGIDFSNMFIQLAGDPKTTLAAAREAGATIAYG
NFITLLINFLIIAWVLFLVVKLMNRLKKREEAKPAPAAPSEEVLLTEIRDILAKQQKA
>Mature_138_residues
MLKEFQEFALKGNMVDLAIGVIIGGAFGGLVNSIVNDIIMPIIGLITGGIDFSNMFIQLAGDPKTTLAAAREAGATIAYG
NFITLLINFLIIAWVLFLVVKLMNRLKKREEAKPAPAAPSEEVLLTEIRDILAKQQKA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=50.3649635036496, Blast_Score=129, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BRUA1 (B2S9G8)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934333.1
- ProteinModelPortal:   B2S9G8
- SMR:   B2S9G8
- GeneID:   6328260
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I03180
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13952
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14899; Mature: 14899

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20aa25c4)-; HASH(0x20ca8ad8)-; HASH(0x207ef028)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFQEFALKGNMVDLAIGVIIGGAFGGLVNSIVNDIIMPIIGLITGGIDFSNMFIQLA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEC
GDPKTTLAAAREAGATIAYGNFITLLINFLIIAWVLFLVVKLMNRLKKREEAKPAPAAPS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEVLLTEIRDILAKQQKA
HHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLKEFQEFALKGNMVDLAIGVIIGGAFGGLVNSIVNDIIMPIIGLITGGIDFSNMFIQLA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEC
GDPKTTLAAAREAGATIAYGNFITLLINFLIIAWVLFLVVKLMNRLKKREEAKPAPAAPS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEVLLTEIRDILAKQQKA
HHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA