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The map label for this gene is gph [H]

Identifier: 225851834

GI number: 225851834

Start: 317576

End: 318325

Strand: Direct

Name: gph [H]

Synonym: BMEA_A0322

Alternate gene names: 225851834

Gene position: 317576-318325 (Clockwise)

Preceding gene: 225851833

Following gene: 225851838

Centisome position: 14.94

GC content: 60.27

Gene sequence:

>750_bases
ATGATGGCTGCGCACCTTGCCGACCGCGTGAAATCGATCCGTGCCGTCCTCTTTGACAAGGACGGCACACTGATCGACTT
CGATCGCACATGGTTCTCCATTTCATGGCAACTGGCGCAATGGTCGGCGCAAGGTGACGAAGTGCTGGCGCGCGCCTTGC
TGGATGCGGGGGGTTATGACTGGCTGGCCGAACGTTTTCGCGCCAATTCCGTCATCGCCGCCGGAACGGTGGAAGATATT
GTTTCGCTTTGGCATCCGGGCCTCGCGGGGCCGCAATTGCGTTCATTGATTGAGAAATATGATGCTTATTGCATCGCCGA
AGGCGCTCGCTCGGCTATCGCTATCGAGGCGGTGCATGAAACGCTTGCCGTCCTGCGCGGTGCGGGCTATCGGCTCGGCA
TTGCCACCAATGATTCCGAAGCAGGAGCCCGTGTCACGGCAAAGGCGCTCGGCATCGACCATCTGTTCGATGTGATGATC
GGCTATGATACCGCCGCGCGCCCGAAACCCTTTCCCGATCCGCTGCTTTATTTTGCCGAAAAGCTTGGGCTCTCGCCGCA
TGAAATTGCCATGGTGGGCGACAATCTGCACGATCTGGAAACCGCCCATGCGGCAGGGGCGGGGCTTGCCGTCGGTGTTC
TTTCCGGCAACAGCCCGCGTGAGGCGCTGGAGCCACATGCCGATCTGGTGCTCGAAAGCGTCGCGGGCCTGCCCGCTATT
CTTCAGCCTTTCGTGCTGCCTTCGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCGTTCGCTGCGCTATGTGCGTCGTGGCGATGATCTTTTGAAGCTCCTGGCTGACAAGCCTTCGGCGGTCAAGAAA
CGCGCCGGTGCGCTTGAAGC

Downstream 100 bases:

>100_bases
TCGGGCATTTTGCCATCAAGCAGCCAGCCAGTCATTTCGGGCCAGAGCGTTTCGGCAAAGCGCGAGCGGAAATAGCCGAG
ATGGCCGATGGGCTGGTTGC

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MMAAHLADRVKSIRAVLFDKDGTLIDFDRTWFSISWQLAQWSAQGDEVLARALLDAGGYDWLAERFRANSVIAAGTVEDI
VSLWHPGLAGPQLRSLIEKYDAYCIAEGARSAIAIEAVHETLAVLRGAGYRLGIATNDSEAGARVTAKALGIDHLFDVMI
GYDTAARPKPFPDPLLYFAEKLGLSPHEIAMVGDNLHDLETAHAAGAGLAVGVLSGNSPREALEPHADLVLESVAGLPAI
LQPFVLPSA

Sequences:

>Translated_249_residues
MMAAHLADRVKSIRAVLFDKDGTLIDFDRTWFSISWQLAQWSAQGDEVLARALLDAGGYDWLAERFRANSVIAAGTVEDI
VSLWHPGLAGPQLRSLIEKYDAYCIAEGARSAIAIEAVHETLAVLRGAGYRLGIATNDSEAGARVTAKALGIDHLFDVMI
GYDTAARPKPFPDPLLYFAEKLGLSPHEIAMVGDNLHDLETAHAAGAGLAVGVLSGNSPREALEPHADLVLESVAGLPAI
LQPFVLPSA
>Mature_249_residues
MMAAHLADRVKSIRAVLFDKDGTLIDFDRTWFSISWQLAQWSAQGDEVLARALLDAGGYDWLAERFRANSVIAAGTVEDI
VSLWHPGLAGPQLRSLIEKYDAYCIAEGARSAIAIEAVHETLAVLRGAGYRLGIATNDSEAGARVTAKALGIDHLFDVMI
GYDTAARPKPFPDPLLYFAEKLGLSPHEIAMVGDNLHDLETAHAAGAGLAVGVLSGNSPREALEPHADLVLESVAGLPAI
LQPFVLPSA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=254, Percent_Identity=30.3149606299213, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71983424, Length=236, Percent_Identity=25.4237288135593, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567717, Length=114, Percent_Identity=35.0877192982456, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26591; Mature: 26591

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAAHLADRVKSIRAVLFDKDGTLIDFDRTWFSISWQLAQWSAQGDEVLARALLDAGGYD
CCHHHHHHHHHHHHHHEECCCCCEEEECCCEEEEEEEEHHCCCCHHHHHHHHHHHCCCHH
WLAERFRANSVIAAGTVEDIVSLWHPGLAGPQLRSLIEKYDAYCIAEGARSAIAIEAVHE
HHHHHHHCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHH
TLAVLRGAGYRLGIATNDSEAGARVTAKALGIDHLFDVMIGYDTAARPKPFPDPLLYFAE
HHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
KLGLSPHEIAMVGDNLHDLETAHAAGAGLAVGVLSGNSPREALEPHADLVLESVAGLPAI
HHCCCHHHEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHHCHHHHHHHHHHCCCHHH
LQPFVLPSA
HHHHCCCCC
>Mature Secondary Structure
MMAAHLADRVKSIRAVLFDKDGTLIDFDRTWFSISWQLAQWSAQGDEVLARALLDAGGYD
CCHHHHHHHHHHHHHHEECCCCCEEEECCCEEEEEEEEHHCCCCHHHHHHHHHHHCCCHH
WLAERFRANSVIAAGTVEDIVSLWHPGLAGPQLRSLIEKYDAYCIAEGARSAIAIEAVHE
HHHHHHHCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHH
TLAVLRGAGYRLGIATNDSEAGARVTAKALGIDHLFDVMIGYDTAARPKPFPDPLLYFAE
HHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
KLGLSPHEIAMVGDNLHDLETAHAAGAGLAVGVLSGNSPREALEPHADLVLESVAGLPAI
HHCCCHHHEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHHCHHHHHHHHHHCCCHHH
LQPFVLPSA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]