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The map label for this gene is mdlA [H]

Identifier: 225851766

GI number: 225851766

Start: 251516

End: 252634

Strand: Direct

Name: mdlA [H]

Synonym: BMEA_A0247

Alternate gene names: 225851766

Gene position: 251516-252634 (Clockwise)

Preceding gene: 225851765

Following gene: 225851767

Centisome position: 11.83

GC content: 59.87

Gene sequence:

>1119_bases
ATGGCAAGGATCGAGCGGTTTGAATTGCGCATGGTGGACCTTCCGCCCAAGGCGAAGCGCACCGATGCAATCCAGAGCTT
TGTAAGCCAGGAAACCCCCATCGTCACCATTACCGACAGCGATGGTGCGGTTGGCACCGGCTATAGCTATACGATTGGCA
CTGGTGGTTCGTCCGTCATGCGCCTGCTTGCCGACCATCTGGCGCCGCTGGTGATTGGCGAGGATGCCGATTGCATCGAG
GCGATCTGGCGCAGGCTGGAATTTGCAACCCATGCCACCACGATCGGCGCAATCACCGCGCTGGCGCTTGCTGCAATCGA
TACCGCGCTTTGGGATCTGCGTGCAAAAAAGCAGAACCTGCCGCTCTGGAAGCTTGCGGGCGGGGCAAGGGACCATTGTC
CGCTTTATACGACCGAAGGCGGCTGGCTGCATATCGAGAAGGAGGCGCTGGTCGAGGATGCGCTTCAAGCCAAGGCGAAA
GGTTTCACCGGCTCCAAGATCAAGATCGGCAAGCCGCATGGCGCGCAGGATTATGCGCGGCTTTCCGCCGTGCGTGCGGC
GGTGGGTGCTGCCTTCGAGATCATGACCGATTGCAATCAGGGTTTTACGGTTGATGAAGCGATCCGCCGTGCCGAGCGCC
TCAAGGAACTTGATCTGGCATGGATCGAGGAGCCGCTACCAGCCGACGATCTCGACGGCCATATGCGCCTGGCCCGCTCC
ACGGCGACGCCCGTTGCAGTGGGTGAATCGATCTACTCGATCCGCCATTTCCGCGAATATATGCACAAGGGTGCCTGCTC
CATCGTGCAGGTGGATGTGGCGCGCATCGGCGGCATCACGCCATGGCTGAAAGTGGCACATATGGCGGAAGCCTTCGATA
TTCCGGTGTGTCCGCATTTCCTGATGGAACTGCATGTAAGCCTCGTCTGTGCGGTGCCGAACGGGCGCTATGTCGAATAT
ATCCCGCAGCTCGATGACCTGACGGACAAGGGTATGGAAATTCGTGACGGCCATGCCGTTGCTCCATCGCAGCCGGGGAT
CGGCATTGCGTGGGATTGGGACGCAGTGCGTTCGCGCTCAATTGGCGAATTCACCCGCGAGATCGTGAGGGCGCCATGA

Upstream 100 bases:

>100_bases
CGCGCCCGGTGCAGCCGGGTGAGAGCATTGCTATGCAGTTCGATCTGGCGCAGGCGCATCTTTTCGATGCCGCTACCGGC
AAGAGTCTGACGGTGTAATC

Downstream 100 bases:

>100_bases
GCAAGCATCAGGGCCAGCGCATGGGGATGGTGATCGGCCTCAACCCGGAAAAGGTCGCGAAATATAAGGCGTTACATGCG
CAGGTCTGGCCGGAAATTCT

Product: mandelate racemase

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MARIERFELRMVDLPPKAKRTDAIQSFVSQETPIVTITDSDGAVGTGYSYTIGTGGSSVMRLLADHLAPLVIGEDADCIE
AIWRRLEFATHATTIGAITALALAAIDTALWDLRAKKQNLPLWKLAGGARDHCPLYTTEGGWLHIEKEALVEDALQAKAK
GFTGSKIKIGKPHGAQDYARLSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEEPLPADDLDGHMRLARS
TATPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWLKVAHMAEAFDIPVCPHFLMELHVSLVCAVPNGRYVEY
IPQLDDLTDKGMEIRDGHAVAPSQPGIGIAWDWDAVRSRSIGEFTREIVRAP

Sequences:

>Translated_372_residues
MARIERFELRMVDLPPKAKRTDAIQSFVSQETPIVTITDSDGAVGTGYSYTIGTGGSSVMRLLADHLAPLVIGEDADCIE
AIWRRLEFATHATTIGAITALALAAIDTALWDLRAKKQNLPLWKLAGGARDHCPLYTTEGGWLHIEKEALVEDALQAKAK
GFTGSKIKIGKPHGAQDYARLSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEEPLPADDLDGHMRLARS
TATPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWLKVAHMAEAFDIPVCPHFLMELHVSLVCAVPNGRYVEY
IPQLDDLTDKGMEIRDGHAVAPSQPGIGIAWDWDAVRSRSIGEFTREIVRAP
>Mature_371_residues
ARIERFELRMVDLPPKAKRTDAIQSFVSQETPIVTITDSDGAVGTGYSYTIGTGGSSVMRLLADHLAPLVIGEDADCIEA
IWRRLEFATHATTIGAITALALAAIDTALWDLRAKKQNLPLWKLAGGARDHCPLYTTEGGWLHIEKEALVEDALQAKAKG
FTGSKIKIGKPHGAQDYARLSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEEPLPADDLDGHMRLARST
ATPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWLKVAHMAEAFDIPVCPHFLMELHVSLVCAVPNGRYVEYI
PQLDDLTDKGMEIRDGHAVAPSQPGIGIAWDWDAVRSRSIGEFTREIVRAP

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=440, Percent_Identity=26.3636363636364, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI42544117, Length=402, Percent_Identity=25.8706467661692, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI186972148, Length=333, Percent_Identity=26.1261261261261, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1787864, Length=367, Percent_Identity=27.2479564032698, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI48994953, Length=276, Percent_Identity=30.0724637681159, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI226510960, Length=350, Percent_Identity=26.2857142857143, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 40745; Mature: 40614

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIERFELRMVDLPPKAKRTDAIQSFVSQETPIVTITDSDGAVGTGYSYTIGTGGSSVM
CCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCHHHHH
RLLADHLAPLVIGEDADCIEAIWRRLEFATHATTIGAITALALAAIDTALWDLRAKKQNL
HHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLWKLAGGARDHCPLYTTEGGWLHIEKEALVEDALQAKAKGFTGSKIKIGKPHGAQDYAR
CEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
LSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEEPLPADDLDGHMRLARS
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TATPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWLKVAHMAEAFDIPVCPHF
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCHHH
LMELHVSLVCAVPNGRYVEYIPQLDDLTDKGMEIRDGHAVAPSQPGIGIAWDWDAVRSRS
HHHHHHEEEEECCCCCEEEECCCHHHHHHCCCEECCCCEECCCCCCCEEEECHHHHHCCC
IGEFTREIVRAP
HHHHHHHHHCCC
>Mature Secondary Structure 
ARIERFELRMVDLPPKAKRTDAIQSFVSQETPIVTITDSDGAVGTGYSYTIGTGGSSVM
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCHHHHH
RLLADHLAPLVIGEDADCIEAIWRRLEFATHATTIGAITALALAAIDTALWDLRAKKQNL
HHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLWKLAGGARDHCPLYTTEGGWLHIEKEALVEDALQAKAKGFTGSKIKIGKPHGAQDYAR
CEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHH
LSAVRAAVGAAFEIMTDCNQGFTVDEAIRRAERLKELDLAWIEEPLPADDLDGHMRLARS
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TATPVAVGESIYSIRHFREYMHKGACSIVQVDVARIGGITPWLKVAHMAEAFDIPVCPHF
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCHHH
LMELHVSLVCAVPNGRYVEYIPQLDDLTDKGMEIRDGHAVAPSQPGIGIAWDWDAVRSRS
HHHHHHEEEEECCCCCEEEECCCHHHHHHCCCEECCCCEECCCCCCCEEEECHHHHHCCC
IGEFTREIVRAP
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]