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The map label for this gene is bioA [C]

Identifier: 225851722

GI number: 225851722

Start: 202031

End: 203401

Strand: Direct

Name: bioA [C]

Synonym: BMEA_A0199

Alternate gene names: 225851722

Gene position: 202031-203401 (Clockwise)

Preceding gene: 225851721

Following gene: 225851724

Centisome position: 9.5

GC content: 59.52

Gene sequence:

>1371_bases
ATGACTGCACAGCCGAACTCGCTTGAAGCCCGCGATATCCGTTATCACCTGCATTCCTACACCGATGCTGTCCGTCTTGA
AGCGGAAGGCCCGCTCGTTATTGAGCGCGGCGACGGCATCTATGTCGAAGATATTGCCGGCAAACGCTATATTGAAGCCA
TGTCGGGCCTGTGGAGTGTCGGCGTTGGTTTTTCGGAACAGCGTCTGGCGGAAGCTGCTGCGCGGCAGATGAAGAAACTG
CCATTTTATCATACCTTTTCCTATCGCTCGCATGGCCCGGTGATCGATCTGGCTGAAAAGCTTGTCGCCATGGCGCCGGT
GCCGATGAGCAAGGCCTATTTCACCAATTCCGGCTCGGAAGCGAATGATACCGTCGTCAAGCTCATCTGGTATCGCTCCA
ATGCGCTGGGCGAGCCTGAGCGCAAGAAGATCATTTCGCGCAAGCGCGGCTATCATGGCGTGACCATTGCCTCCGCCAGC
CTGACGGGCCTGCCCAACAATCACCGCTCCTTCGATCTGCCCATCGATCGTATTCTTCATACCGGCTGCCCGCATCATTA
CCACGATGCGCTGCCGGGTGAGAGCGAGGAGCAGTTCGCAACGCGCCTGGCAAACGAGCTGGAACAGCTCATCCTGGCGG
AAGGCCCCCACACCATTGCCGCTTTCATCGGCGAGCCGGTGATGGGCGCAGGCGGCGTGGTCGTGCCGCCGAAGACATAT
TGGGAAAAGGTGCAGGCAGTTCTCGGCCGCTACAATATCTTGCTGGTGGCTGATGAAGTCATCTGCGGCTTCGGCCGCAC
GGGCAATCTCTTCGGCTGCCAGACTTTCGACATCAAGCCCGACATTCTGGTCATGTCGAAGCAGCTTTCCTCTTCTTATC
TGCCGATTTCTGCCTTCCTTATCAACGAGCGCGTCTATGCGCCAATTGCTGAGGAAAGCCACAAGATCGGCACGCTTGGC
ACCGGCTTCACCGCATCGGGGCATCCGGTTGCCGCGGCCGTAGCGCTGGAAAACCTCGCCATCATCGAGGAGCGTGATCT
GGTCGCCAATGCGCGTGAGCGCGGCGCGCGGATGCAAAAACGCCTTCGCGAGCTTCAGGACCATCCGCTGGTGGGAGAAG
TGCGCGGCGTCGGCCTTATCGCCGGCGTTGAACTGGTGATCGACAAGGAAGCCAAAACCGGGCTTGAACAGCCGGGTGCG
CTTGGCGCACGGGCGAATGCTGCCTTGCAGGAGCGCGGCGTCATTTCCCGCGCCATGGGCGACACGCTCGCCTTCTGCCC
GCCGCTCATCATCAACGACCAGCAGGTTGATACCATGGTTTCGGCGCTGGAAGCAGCCCTGAACGACGTTCAGGCGAGCC
TTGGAAAGTAA

Upstream 100 bases:

>100_bases
CCTCGGCGGTTTGTACCGACGGGCATTTTCTGCGGCTTTCCGGTTTGCGCTTTCCCTGAAAAAGGCTACACATTCATTAT
CGTTCTTGGAGGATGAATGA

Downstream 100 bases:

>100_bases
TTCCGGACGCACAATCTCGCACTTGCGGGCCGCTCTGTTTCAGGAGCGGCCCGTGCCCAAGCCAGGTAAATAGCATAATT
TTTATAGGATTTTCCGTGGC

Product: aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTAQPNSLEARDIRYHLHSYTDAVRLEAEGPLVIERGDGIYVEDIAGKRYIEAMSGLWSVGVGFSEQRLAEAAARQMKKL
PFYHTFSYRSHGPVIDLAEKLVAMAPVPMSKAYFTNSGSEANDTVVKLIWYRSNALGEPERKKIISRKRGYHGVTIASAS
LTGLPNNHRSFDLPIDRILHTGCPHHYHDALPGESEEQFATRLANELEQLILAEGPHTIAAFIGEPVMGAGGVVVPPKTY
WEKVQAVLGRYNILLVADEVICGFGRTGNLFGCQTFDIKPDILVMSKQLSSSYLPISAFLINERVYAPIAEESHKIGTLG
TGFTASGHPVAAAVALENLAIIEERDLVANARERGARMQKRLRELQDHPLVGEVRGVGLIAGVELVIDKEAKTGLEQPGA
LGARANAALQERGVISRAMGDTLAFCPPLIINDQQVDTMVSALEAALNDVQASLGK

Sequences:

>Translated_456_residues
MTAQPNSLEARDIRYHLHSYTDAVRLEAEGPLVIERGDGIYVEDIAGKRYIEAMSGLWSVGVGFSEQRLAEAAARQMKKL
PFYHTFSYRSHGPVIDLAEKLVAMAPVPMSKAYFTNSGSEANDTVVKLIWYRSNALGEPERKKIISRKRGYHGVTIASAS
LTGLPNNHRSFDLPIDRILHTGCPHHYHDALPGESEEQFATRLANELEQLILAEGPHTIAAFIGEPVMGAGGVVVPPKTY
WEKVQAVLGRYNILLVADEVICGFGRTGNLFGCQTFDIKPDILVMSKQLSSSYLPISAFLINERVYAPIAEESHKIGTLG
TGFTASGHPVAAAVALENLAIIEERDLVANARERGARMQKRLRELQDHPLVGEVRGVGLIAGVELVIDKEAKTGLEQPGA
LGARANAALQERGVISRAMGDTLAFCPPLIINDQQVDTMVSALEAALNDVQASLGK
>Mature_455_residues
TAQPNSLEARDIRYHLHSYTDAVRLEAEGPLVIERGDGIYVEDIAGKRYIEAMSGLWSVGVGFSEQRLAEAAARQMKKLP
FYHTFSYRSHGPVIDLAEKLVAMAPVPMSKAYFTNSGSEANDTVVKLIWYRSNALGEPERKKIISRKRGYHGVTIASASL
TGLPNNHRSFDLPIDRILHTGCPHHYHDALPGESEEQFATRLANELEQLILAEGPHTIAAFIGEPVMGAGGVVVPPKTYW
EKVQAVLGRYNILLVADEVICGFGRTGNLFGCQTFDIKPDILVMSKQLSSSYLPISAFLINERVYAPIAEESHKIGTLGT
GFTASGHPVAAAVALENLAIIEERDLVANARERGARMQKRLRELQDHPLVGEVRGVGLIAGVELVIDKEAKTGLEQPGAL
GARANAALQERGVISRAMGDTLAFCPPLIINDQQVDTMVSALEAALNDVQASLGK

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=470, Percent_Identity=28.0851063829787, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI37574042, Length=441, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI24119277, Length=433, Percent_Identity=28.8683602771363, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI226442705, Length=441, Percent_Identity=28.1179138321995, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI4557809, Length=430, Percent_Identity=27.906976744186, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI226442709, Length=407, Percent_Identity=28.7469287469287, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI284507298, Length=323, Percent_Identity=28.1733746130031, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786991, Length=443, Percent_Identity=30.2483069977427, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788044, Length=422, Percent_Identity=29.3838862559242, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI145693181, Length=417, Percent_Identity=27.5779376498801, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=27.6442307692308, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789759, Length=407, Percent_Identity=27.7641277641278, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787560, Length=416, Percent_Identity=28.3653846153846, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564660, Length=451, Percent_Identity=29.2682926829268, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=445, Percent_Identity=27.6404494382022, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI71992977, Length=438, Percent_Identity=25.3424657534247, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=252, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=467, Percent_Identity=21.1991434689507, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=433, Percent_Identity=28.6374133949192, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324432, Length=432, Percent_Identity=26.6203703703704, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324386, Length=434, Percent_Identity=26.7281105990783, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=291, Percent_Identity=25.085910652921, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI161085790, Length=445, Percent_Identity=31.0112359550562, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28574759, Length=448, Percent_Identity=30.5803571428571, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21357415, Length=436, Percent_Identity=28.6697247706422, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21356575, Length=428, Percent_Identity=27.5700934579439, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI281366494, Length=454, Percent_Identity=23.1277533039648, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667139, Length=454, Percent_Identity=23.1277533039648, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667143, Length=454, Percent_Identity=23.1277533039648, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49594; Mature: 49463

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQPNSLEARDIRYHLHSYTDAVRLEAEGPLVIERGDGIYVEDIAGKRYIEAMSGLWSV
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
GVGFSEQRLAEAAARQMKKLPFYHTFSYRSHGPVIDLAEKLVAMAPVPMSKAYFTNSGSE
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHEEECCCCC
ANDTVVKLIWYRSNALGEPERKKIISRKRGYHGVTIASASLTGLPNNHRSFDLPIDRILH
CCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECEECCCCCCCCEECCCHHHHHH
TGCPHHYHDALPGESEEQFATRLANELEQLILAEGPHTIAAFIGEPVMGAGGVVVPPKTY
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEECCHHH
WEKVQAVLGRYNILLVADEVICGFGRTGNLFGCQTFDIKPDILVMSKQLSSSYLPISAFL
HHHHHHHHCCCEEEEEECHHHHCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCCCEEEEE
INERVYAPIAEESHKIGTLGTGFTASGHPVAAAVALENLAIIEERDLVANARERGARMQK
ECCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
RLRELQDHPLVGEVRGVGLIAGVELVIDKEAKTGLEQPGALGARANAALQERGVISRAMG
HHHHHHCCCCEEHHCCCEEEECEEEEEECHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHC
DTLAFCPPLIINDQQVDTMVSALEAALNDVQASLGK
CHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQPNSLEARDIRYHLHSYTDAVRLEAEGPLVIERGDGIYVEDIAGKRYIEAMSGLWSV
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
GVGFSEQRLAEAAARQMKKLPFYHTFSYRSHGPVIDLAEKLVAMAPVPMSKAYFTNSGSE
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHEEECCCCC
ANDTVVKLIWYRSNALGEPERKKIISRKRGYHGVTIASASLTGLPNNHRSFDLPIDRILH
CCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECEECCCCCCCCEECCCHHHHHH
TGCPHHYHDALPGESEEQFATRLANELEQLILAEGPHTIAAFIGEPVMGAGGVVVPPKTY
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEECCHHH
WEKVQAVLGRYNILLVADEVICGFGRTGNLFGCQTFDIKPDILVMSKQLSSSYLPISAFL
HHHHHHHHCCCEEEEEECHHHHCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCCCEEEEE
INERVYAPIAEESHKIGTLGTGFTASGHPVAAAVALENLAIIEERDLVANARERGARMQK
ECCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
RLRELQDHPLVGEVRGVGLIAGVELVIDKEAKTGLEQPGALGARANAALQERGVISRAMG
HHHHHHCCCCEEHHCCCEEEECEEEEEECHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHC
DTLAFCPPLIINDQQVDTMVSALEAALNDVQASLGK
CHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]