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The map label for this gene is cobA [H]

Identifier: 225851711

GI number: 225851711

Start: 190162

End: 191568

Strand: Direct

Name: cobA [H]

Synonym: BMEA_A0186

Alternate gene names: 225851711

Gene position: 190162-191568 (Clockwise)

Preceding gene: 225851710

Following gene: 225851712

Centisome position: 8.95

GC content: 63.97

Gene sequence:

>1407_bases
GTGTCTTTATCGCAAACGCGACTTCTGGCTGATTTTCCTGCTTTCTTCCGGGTTTCGGGCGAAGTGGTGGCGGTCGTGGG
TGGTGGCGAGGAAGCCTTGAACAAGGCTCGCCTCGTGGCGCAGACGGCTGCGCGCCTGCGCATCATTGCTGAAAAGGCTG
AGCCGCATCTTGCCGCTTTTATTGAAAGCCATGGGGTGGAGCATATTGCGTCACCCTTTGCGCCGGAACTGCTGGATGGT
GCGAAGCTCGTTTTTGTCGCGACCGGCGATGAGGCCGGGGATCGTGCCGCCGCTGCCGCCGCAAGGGCCCGCAAAATTCC
GGTCAATGTGGTGGACCGTCCCGCCCTTTGCGATTTTCTGACACCTGCCATCGTCAATCGTGCGCCGGTCACGATCGCCA
TCGGTTCGCAGGGAACCGCCCCGGTTTTGGCGCAGATGGTGCGTGCGCGTATCGATGCAGCCTTTTCGCCGCGTCTTGGC
GAGCTTGCGCATTTTGCGGAAAGCTGGCGCCCTCTGGTCGAGCGGGCGCTTCCAAAAGGGCTTGCGCGGCGTGGTTTCTG
GCGCACATTCTTTTCGGGAAAAGTGGCCCGCGCGGTGGAGAGTGGCGACCACGAGGCGGCGCACAGGGCTGCGGCGGAAC
TGATCGGGCAGGGGCAGGACGCTTCCGGCCATGTCTGGCTGGTTGGGGCCGGTCCCGGTGCTGAAGACCTTTTGACGCTG
CGCGCCCAGCGCGTGCTGATGGAAGCCGATGTGATCGTTCATGATGCGCTGGTGCCGGAAGGCGTCATCGCCATGGGACG
CCGCGACGCCGAGCGCCTGCCGGTAGGCAAGCGCAAGGGTTGCCATTCCAAAAGCCAGGGTGAGATCAACCGCCTGCTGG
TGAAGCTGGGGCAGGAGGGCAAGCGCGTGGTGCGCCTGAAATCCGGCGATCCGCTGGTCTTTGGCCGTGCGGGTGAGGAA
ATGGCCGCCTTGCGCGCCGCCGGAATAGGCTTTGAGGTGGTGCCGGGCATCACCTCTGCCTTTGCCGCTGCCGCCGATAT
GGAACTGCCGCTGACCTTGCGCGGCGTCGCGTCCTCGCTCGTCTTCACCACGGGCCATGACATGACCGGCGATGTGCTGC
CCGGCTGGGCGAAGCTTGCCGTCTATATGGGCTCCACGGTTGCCGCTTCCGTTGCCTCGCGTCTGATTGCCGCCGGCCTG
CATGAGGATACGGCGGTTGCAGTGGTGGAAAATGCAAGCCGCAGCAACAAGCGCATGTTCCATGGCACGCTGAAGGATTT
GCCGCAGCTTGAAAACCGCAAGGAGCTTTCCGGCCCGGTCATGGTGGTCATTGGCGATGCCGTCGCCGGTGCCGCCATCG
GCAGGGCGCAGGCCCTTTCCCTGAAACCTGTTCCCGTCGCAGCCTGA

Upstream 100 bases:

>100_bases
CTTATAAATTCCCCCCGCGCAATGAATAATAGCGGCCATCTTGCTTCCAGCGCCAGCAGGCGTCTGCTCCGTGCTGGAAG
TGTTTGAAAGGATGATGCCC

Downstream 100 bases:

>100_bases
TTTTGAGGAGTTTGGATATGATCGTGAAAGTTCTTACTGCAAACCGGCTGATCGATGGACAGGCTGTGTGGCTTGGTGCC
GACGGTTCGTGGCAGGAAAC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSLSQTRLLADFPAFFRVSGEVVAVVGGGEEALNKARLVAQTAARLRIIAEKAEPHLAAFIESHGVEHIASPFAPELLDG
AKLVFVATGDEAGDRAAAAAARARKIPVNVVDRPALCDFLTPAIVNRAPVTIAIGSQGTAPVLAQMVRARIDAAFSPRLG
ELAHFAESWRPLVERALPKGLARRGFWRTFFSGKVARAVESGDHEAAHRAAAELIGQGQDASGHVWLVGAGPGAEDLLTL
RAQRVLMEADVIVHDALVPEGVIAMGRRDAERLPVGKRKGCHSKSQGEINRLLVKLGQEGKRVVRLKSGDPLVFGRAGEE
MAALRAAGIGFEVVPGITSAFAAAADMELPLTLRGVASSLVFTTGHDMTGDVLPGWAKLAVYMGSTVAASVASRLIAAGL
HEDTAVAVVENASRSNKRMFHGTLKDLPQLENRKELSGPVMVVIGDAVAGAAIGRAQALSLKPVPVAA

Sequences:

>Translated_468_residues
MSLSQTRLLADFPAFFRVSGEVVAVVGGGEEALNKARLVAQTAARLRIIAEKAEPHLAAFIESHGVEHIASPFAPELLDG
AKLVFVATGDEAGDRAAAAAARARKIPVNVVDRPALCDFLTPAIVNRAPVTIAIGSQGTAPVLAQMVRARIDAAFSPRLG
ELAHFAESWRPLVERALPKGLARRGFWRTFFSGKVARAVESGDHEAAHRAAAELIGQGQDASGHVWLVGAGPGAEDLLTL
RAQRVLMEADVIVHDALVPEGVIAMGRRDAERLPVGKRKGCHSKSQGEINRLLVKLGQEGKRVVRLKSGDPLVFGRAGEE
MAALRAAGIGFEVVPGITSAFAAAADMELPLTLRGVASSLVFTTGHDMTGDVLPGWAKLAVYMGSTVAASVASRLIAAGL
HEDTAVAVVENASRSNKRMFHGTLKDLPQLENRKELSGPVMVVIGDAVAGAAIGRAQALSLKPVPVAA
>Mature_467_residues
SLSQTRLLADFPAFFRVSGEVVAVVGGGEEALNKARLVAQTAARLRIIAEKAEPHLAAFIESHGVEHIASPFAPELLDGA
KLVFVATGDEAGDRAAAAAARARKIPVNVVDRPALCDFLTPAIVNRAPVTIAIGSQGTAPVLAQMVRARIDAAFSPRLGE
LAHFAESWRPLVERALPKGLARRGFWRTFFSGKVARAVESGDHEAAHRAAAELIGQGQDASGHVWLVGAGPGAEDLLTLR
AQRVLMEADVIVHDALVPEGVIAMGRRDAERLPVGKRKGCHSKSQGEINRLLVKLGQEGKRVVRLKSGDPLVFGRAGEEM
AALRAAGIGFEVVPGITSAFAAAADMELPLTLRGVASSLVFTTGHDMTGDVLPGWAKLAVYMGSTVAASVASRLIAAGLH
EDTAVAVVENASRSNKRMFHGTLKDLPQLENRKELSGPVMVVIGDAVAGAAIGRAQALSLKPVPVAA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=445, Percent_Identity=38.876404494382, Blast_Score=301, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6322922, Length=237, Percent_Identity=30.379746835443, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 49071; Mature: 48940

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2 ; PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSQTRLLADFPAFFRVSGEVVAVVGGGEEALNKARLVAQTAARLRIIAEKAEPHLAAF
CCCCHHHHHHHCCHHHEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IESHGVEHIASPFAPELLDGAKLVFVATGDEAGDRAAAAAARARKIPVNVVDRPALCDFL
HHHCCHHHHHCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
TPAIVNRAPVTIAIGSQGTAPVLAQMVRARIDAAFSPRLGELAHFAESWRPLVERALPKG
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LARRGFWRTFFSGKVARAVESGDHEAAHRAAAELIGQGQDASGHVWLVGAGPGAEDLLTL
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
RAQRVLMEADVIVHDALVPEGVIAMGRRDAERLPVGKRKGCHSKSQGEINRLLVKLGQEG
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
KRVVRLKSGDPLVFGRAGEEMAALRAAGIGFEVVPGITSAFAAAADMELPLTLRGVASSL
CEEEEEECCCCEEEECCCHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCEEHHHHHHHH
VFTTGHDMTGDVLPGWAKLAVYMGSTVAASVASRLIAAGLHEDTAVAVVENASRSNKRMF
EEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
HGTLKDLPQLENRKELSGPVMVVIGDAVAGAAIGRAQALSLKPVPVAA
HHHHHHHHHHCCCHHCCCCEEEEEECHHHHHHHCCHHEEECCCCCCCC
>Mature Secondary Structure 
SLSQTRLLADFPAFFRVSGEVVAVVGGGEEALNKARLVAQTAARLRIIAEKAEPHLAAF
CCCHHHHHHHCCHHHEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IESHGVEHIASPFAPELLDGAKLVFVATGDEAGDRAAAAAARARKIPVNVVDRPALCDFL
HHHCCHHHHHCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
TPAIVNRAPVTIAIGSQGTAPVLAQMVRARIDAAFSPRLGELAHFAESWRPLVERALPKG
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LARRGFWRTFFSGKVARAVESGDHEAAHRAAAELIGQGQDASGHVWLVGAGPGAEDLLTL
HHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
RAQRVLMEADVIVHDALVPEGVIAMGRRDAERLPVGKRKGCHSKSQGEINRLLVKLGQEG
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
KRVVRLKSGDPLVFGRAGEEMAALRAAGIGFEVVPGITSAFAAAADMELPLTLRGVASSL
CEEEEEECCCCEEEECCCHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCCEEHHHHHHHH
VFTTGHDMTGDVLPGWAKLAVYMGSTVAASVASRLIAAGLHEDTAVAVVENASRSNKRMF
EEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
HGTLKDLPQLENRKELSGPVMVVIGDAVAGAAIGRAQALSLKPVPVAA
HHHHHHHHHHCCCHHCCCCEEEEEECHHHHHHHCCHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA