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The map label for this gene is ysgA [H]

Identifier: 225851684

GI number: 225851684

Start: 167620

End: 168513

Strand: Reverse

Name: ysgA [H]

Synonym: BMEA_A0156

Alternate gene names: 225851684

Gene position: 168513-167620 (Counterclockwise)

Preceding gene: 225851685

Following gene: 225851683

Centisome position: 7.93

GC content: 60.29

Gene sequence:

>894_bases
ATGAGCCGTAACGACGAATTCTCCAGAAGCGGCGGCTCGCGCCCCGAACACCGGTCCGAACACCCGAAGGTCGGCCAGGT
AAAGGAAGTCACCAGCCTTGCCAACCCGATCGTGAAGGACCTGCGTTCACTGGCGCTGAAAAAATTTCGCGACCAGCAGG
GCGTATTTCTGGCCGAAGGGCTGAAGCTTGTCATCGACGCGCTGGAGCAGGACTGGCGCATCAAGACGCTGGTTTTCGCC
AAGTCCGGCAAGGGCAACAAAACCGTGGAACAGGTCGCCGCACGCACCTTTGCCAAGGGCGGGCTGGTGCTGGAAGTGAC
TGAAAAGGTCATCTCCGCCATTACGCGCCGCGATAATCCGCAGATGGTGGCTGGCGTTTTCGAGCAGCAATACCACCAGC
TTGCGGGCCTGAAGCCGAAAGGCAACGATGTTTATGTCGCGCTTGATCGCGTGCGCGATCCCGGCAATCTTGGCGCCGTC
ATCCGCACAGCCGATGCGGTCGGCGCAAAGGGCGTGATCCTCATTGGCGACACCACCGATCCCTATTCGCTCGAAACCGT
GCGCGCCACCATGGGCTCGGTCTTTTCCGTGCCGCTCTACAGGGCTTCGGAAGCCGATTTTCTCAACTGGCGCAAGGGCT
TTTCTGGCCTCGTCGTCGGCACGCACCTGAAAGGCGCCGTGGATTATCGCACCATCCCCTATGCCAACAAGCCGGTTATC
CTGATAATGGGCAATGAACAGCAGGGCCTGCCCGACAGTCTTGCCGGCGCCTGCGACAAGCTTGCCCGCATTCCGCAGGC
AGGCCGCGCCGATTCGCTCAATCTGGCCATTGCCACAGGTGTGATGCTCTATGAAATCCGCCGTGAGGCACTCACCCTCG
ATGAAAGGGGATAG

Upstream 100 bases:

>100_bases
GCACGGTCGAATCAGGCGAACTCATCCTGCGCGAACGCTCCGCCGATCAGTCGGGAGGGCGCGCGCTTTCCACATCCATG
TTCAGCCGCTGGGTAGCCAA

Downstream 100 bases:

>100_bases
GATGAAGCGTCACGCAGTCTGGTCCTCGCTTTTCGTCGTCATTCTTGCAGTCCTGATTGACCAGGGCATCAAATATCTGG
TCGAAAGCCGCATGTTCTAT

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSRNDEFSRSGGSRPEHRSEHPKVGQVKEVTSLANPIVKDLRSLALKKFRDQQGVFLAEGLKLVIDALEQDWRIKTLVFA
KSGKGNKTVEQVAARTFAKGGLVLEVTEKVISAITRRDNPQMVAGVFEQQYHQLAGLKPKGNDVYVALDRVRDPGNLGAV
IRTADAVGAKGVILIGDTTDPYSLETVRATMGSVFSVPLYRASEADFLNWRKGFSGLVVGTHLKGAVDYRTIPYANKPVI
LIMGNEQQGLPDSLAGACDKLARIPQAGRADSLNLAIATGVMLYEIRREALTLDERG

Sequences:

>Translated_297_residues
MSRNDEFSRSGGSRPEHRSEHPKVGQVKEVTSLANPIVKDLRSLALKKFRDQQGVFLAEGLKLVIDALEQDWRIKTLVFA
KSGKGNKTVEQVAARTFAKGGLVLEVTEKVISAITRRDNPQMVAGVFEQQYHQLAGLKPKGNDVYVALDRVRDPGNLGAV
IRTADAVGAKGVILIGDTTDPYSLETVRATMGSVFSVPLYRASEADFLNWRKGFSGLVVGTHLKGAVDYRTIPYANKPVI
LIMGNEQQGLPDSLAGACDKLARIPQAGRADSLNLAIATGVMLYEIRREALTLDERG
>Mature_296_residues
SRNDEFSRSGGSRPEHRSEHPKVGQVKEVTSLANPIVKDLRSLALKKFRDQQGVFLAEGLKLVIDALEQDWRIKTLVFAK
SGKGNKTVEQVAARTFAKGGLVLEVTEKVISAITRRDNPQMVAGVFEQQYHQLAGLKPKGNDVYVALDRVRDPGNLGAVI
RTADAVGAKGVILIGDTTDPYSLETVRATMGSVFSVPLYRASEADFLNWRKGFSGLVVGTHLKGAVDYRTIPYANKPVIL
IMGNEQQGLPDSLAGACDKLARIPQAGRADSLNLAIATGVMLYEIRREALTLDERG

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=172, Percent_Identity=31.9767441860465, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790623, Length=146, Percent_Identity=35.6164383561644, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666840, Length=291, Percent_Identity=23.3676975945017, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 32342; Mature: 32210

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNDEFSRSGGSRPEHRSEHPKVGQVKEVTSLANPIVKDLRSLALKKFRDQQGVFLAEG
CCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
LKLVIDALEQDWRIKTLVFAKSGKGNKTVEQVAARTFAKGGLVLEVTEKVISAITRRDNP
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCC
QMVAGVFEQQYHQLAGLKPKGNDVYVALDRVRDPGNLGAVIRTADAVGAKGVILIGDTTD
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEHHCCCCCEEEEECCCCC
PYSLETVRATMGSVFSVPLYRASEADFLNWRKGFSGLVVGTHLKGAVDYRTIPYANKPVI
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCEE
LIMGNEQQGLPDSLAGACDKLARIPQAGRADSLNLAIATGVMLYEIRREALTLDERG
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRNDEFSRSGGSRPEHRSEHPKVGQVKEVTSLANPIVKDLRSLALKKFRDQQGVFLAEG
CCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
LKLVIDALEQDWRIKTLVFAKSGKGNKTVEQVAARTFAKGGLVLEVTEKVISAITRRDNP
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCC
QMVAGVFEQQYHQLAGLKPKGNDVYVALDRVRDPGNLGAVIRTADAVGAKGVILIGDTTD
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEHHCCCCCEEEEECCCCC
PYSLETVRATMGSVFSVPLYRASEADFLNWRKGFSGLVVGTHLKGAVDYRTIPYANKPVI
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEEECCCCCCCEE
LIMGNEQQGLPDSLAGACDKLARIPQAGRADSLNLAIATGVMLYEIRREALTLDERG
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]