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The map label for this gene is trpS [H]

Identifier: 225851677

GI number: 225851677

Start: 154766

End: 155923

Strand: Reverse

Name: trpS [H]

Synonym: BMEA_A0148

Alternate gene names: 225851677

Gene position: 155923-154766 (Counterclockwise)

Preceding gene: 225851678

Following gene: 225851676

Centisome position: 7.34

GC content: 59.33

Gene sequence:

>1158_bases
TTGAAAGCCTGCCATGCGTCTGCGAAAACACGCCGCATCATTATGACCCTTCGCTCTCCACCGGCGCAGGTCAACCTTCA
GGAACCTATCATGAGCACGTTCAAACCGCTTGTCTTCTCCGGCGTCCAACCGACCGGAAATCTTCACCTCGGCAATTATC
TCGGGGCGATCAAGCGGTGGGTCGAAGTCCAGAAGACCGAGGAATGCATCTATTGCGTCGTGGACATGCACGCGCTGACC
GTTTCGCCCGATCCGGTTGAGCTGATGCAGTCGACCCGCGAAGTCACCGCCGCTTTTCTCGCCGCCGGCATCGACCCGAA
GAAGAGCATCGTCTTCAACCAGAGTCGCGTCATGCAGCATGCGGAGCTTGCCTGGGTGTTCAACTGCGTCGCGCGCATTG
GCTGGATGAGCCGCATGACCCAGTTCAAGGACAAGGCAGGCAAGGATCGCGAGAATGCCTCGCTCGGTCTTTTTGCCTAT
CCGAGCCTGATGGCCGCCGATATTCTGCTTTATCGCGCAACCGCAGTGCCGGTTGGCGAGGACCAGAAGCAGCATCTGGA
GCTGACCCGCGATATTGCGCAGAAGTTCAACAACGACTATTCCGACCGCATCGCCTCGCTTGGTGTGGGCGTCGATATGA
AAGTGGGCGACGAGCAGGTTTCAGGCTTCTTCCCGCTGACGGAACCGATGATCTCTGGCCCCGCCATGCGCATCATGTCG
CTGCGCGACGGCACCAAGAAAATGTCGAAGTCCGATCCGTCCGACCTGTCACGCATCAACCTGATCGACGATGAGGACAC
GATCACCAAGAAAATCCGCAAGGCCAAGACCGATTCCGACGGCCTGCCCTCAGAAGTGGACGGGCTGGAAGGCCGCCCGG
AGGCCGATAATCTCGTCGGCATTTACGCCGCCCTCTCCTCGACCACCAAGGAAGATGTGCTGAAGGAATTCGGCGGACGC
CAGTTCTCCGACCTGAAGGCCTCGCTTGCCGATCTCGCCGTTGCCAGGCTTTCGCCGATCACGCATGAAATGCGTCGCCT
GGTGGCCGATCCGGCCCATATCGACAGCGTTCTGCGTGATGGCGGTGAGCAGGCAGGCGCGATTGCCGAACAGACCATGC
GTCATGTCCGCGATATTGTCGGCTGGTTGCAGAACTGA

Upstream 100 bases:

>100_bases
GCTCGCTTTCGGACTTGGCGGGGCCAATACAGGCATGATCCGGCGCAACCTCAAGCGCGGCGGGGGCAAAAATCAGCCGA
AAAGCGAAGCATAATTAAGC

Downstream 100 bases:

>100_bases
TTAAAAATGGAAGGCTGCCCGCTTGCGGGCAGCTTTCCGCGATGGCACATTGCCGCCATGGTATCCAGACGACTTAGCCG
CGAAGCCGGCCACCGACGCA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKACHASAKTRRIIMTLRSPPAQVNLQEPIMSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALT
VSPDPVELMQSTREVTAAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAY
PSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMS
LRDGTKKMSKSDPSDLSRINLIDDEDTITKKIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGR
QFSDLKASLADLAVARLSPITHEMRRLVADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN

Sequences:

>Translated_385_residues
MKACHASAKTRRIIMTLRSPPAQVNLQEPIMSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALT
VSPDPVELMQSTREVTAAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAY
PSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMS
LRDGTKKMSKSDPSDLSRINLIDDEDTITKKIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGR
QFSDLKASLADLAVARLSPITHEMRRLVADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN
>Mature_385_residues
MKACHASAKTRRIIMTLRSPPAQVNLQEPIMSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALT
VSPDPVELMQSTREVTAAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAY
PSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMS
LRDGTKKMSKSDPSDLSRINLIDDEDTITKKIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGR
QFSDLKASLADLAVARLSPITHEMRRLVADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=351, Percent_Identity=39.8860398860399, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI41352700, Length=199, Percent_Identity=45.7286432160804, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1789786, Length=349, Percent_Identity=42.9799426934097, Blast_Score=300, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI71982800, Length=365, Percent_Identity=35.0684931506849, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71982793, Length=365, Percent_Identity=35.0684931506849, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320474, Length=364, Percent_Identity=37.0879120879121, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24666151, Length=347, Percent_Identity=36.3112391930836, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 42474; Mature: 42474

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKACHASAKTRRIIMTLRSPPAQVNLQEPIMSTFKPLVFSGVQPTGNLHLGNYLGAIKRW
CCCCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
VEVQKTEECIYCVVDMHALTVSPDPVELMQSTREVTAAFLAAGIDPKKSIVFNQSRVMQH
HHHHHHHHHEEEEEEHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECHHHHHHH
AELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAYPSLMAADILLYRATAVPVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
DQKQHLELTRDIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMS
CHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECHHHHCCCCCCCHHHHCCCHHHHHH
LRDGTKKMSKSDPSDLSRINLIDDEDTITKKIRKAKTDSDGLPSEVDGLEGRPEADNLVG
HHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHH
IYAALSSTTKEDVLKEFGGRQFSDLKASLADLAVARLSPITHEMRRLVADPAHIDSVLRD
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
GGEQAGAIAEQTMRHVRDIVGWLQN
CCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKACHASAKTRRIIMTLRSPPAQVNLQEPIMSTFKPLVFSGVQPTGNLHLGNYLGAIKRW
CCCCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
VEVQKTEECIYCVVDMHALTVSPDPVELMQSTREVTAAFLAAGIDPKKSIVFNQSRVMQH
HHHHHHHHHEEEEEEHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECHHHHHHH
AELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAYPSLMAADILLYRATAVPVGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
DQKQHLELTRDIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMS
CHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECHHHHCCCCCCCHHHHCCCHHHHHH
LRDGTKKMSKSDPSDLSRINLIDDEDTITKKIRKAKTDSDGLPSEVDGLEGRPEADNLVG
HHHHHHHHCCCCCCHHHEEECCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHH
IYAALSSTTKEDVLKEFGGRQFSDLKASLADLAVARLSPITHEMRRLVADPAHIDSVLRD
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
GGEQAGAIAEQTMRHVRDIVGWLQN
CCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]