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The map label for this gene is rlmN [H]

Identifier: 225851618

GI number: 225851618

Start: 84622

End: 85857

Strand: Reverse

Name: rlmN [H]

Synonym: BMEA_A0080

Alternate gene names: 225851618

Gene position: 85857-84622 (Counterclockwise)

Preceding gene: 225851619

Following gene: 225851617

Centisome position: 4.04

GC content: 59.14

Gene sequence:

>1236_bases
ATGTCCATTTCGTTCGACCTTACCATTGATGATACGCGCGACCAGCTTGCCCGCCATGCGCGCGCCAGCCTGGAGGCAAA
GCCGTCGCTCATCGGCATGTCGCGCGAGGAAATGGCCGCAGCACTGATTGCAGCCGGGGTGCCGGAACGTCAGGTGAAGA
TGCGCATCAGCCAGCTCTGGCATTGGCTCTATGTACGCGGCGTTTCCGATTTCGCCGACATGCGCAATATTTCCAAGGAT
CTGCGCGCGATGCTGGCGCAGCATTTCACCATTGCGCGCCCGGAAGTGGTTGAGGAACAGATTTCGCAGGACGGCACACG
CAAATGGCTGTTCCGCTTTCCGCCGCGCAGCGCTGGCCGCCCCGTCGAGATTGAGAGCGTCTATATCCCCGAAGAAGGGC
GCGGCACGCTCTGCATTTCCTCACAGGTCGGCTGCACGCTCACCTGCTCCTTCTGCCATACCGGCACGCAGAAGCTGGTG
CGCAACCTCACCTCGGAAGAAATTCTGGCGCAGCTTTTGACCGCGCGCGACAGGCTTGGTGATTTTCCGGACAAGGATAC
GCCCGACGGCGCCATGGTGCCTGCCGAGGGACGCAAGATCACCAATATCGTGATGATGGGCATGGGCGAGCCGCTCTATA
ATTTCGAGGAAGTGAAGAAGGCCCTGCTGATCGCCTCCGATGGTGACGGCCTGTCCCTTTCCAAGCGCCGCATCACGCTT
TCAACCTCCGGCGTCGTGCCGGAGATTTATCGCACCGGCGACGAGATTGGCGTCATGCTCGCCATTTCGCTACATGCCGT
GCGCGACGAGTTGCGCGACATTCTGGTGCCGATCAACAAAAAATATCCGCTGGCTGAGCTCATCAAAGCCTGCCGCGAAT
ATCCGGGCCTGTCGAACGCCAAGCGCATCACGTTTGAATATGTGATGCTGAAGGATATCAACGACAGTCTCGACGATGCC
AAGCTTCTGGTGAAGCTGTTGCAGGGCATTCCGGCCAAGATCAACCTGATCCCCTTCAACCCGTGGCCCGGCACGAATTA
CCAGTGCTCGGACTGGGAGCAGATCGAGAAATTTGCCGATTATGTAAACGCGGCAGGCTATGCATCGCCGATCCGCACCC
CGCGCGGACGCGATATTCTTGCCGCCTGCGGCCAGTTGAAGTCGGAATCCGAACGCCTGCGCAAGAGCGAACGCCTCGCT
CTCGAAGCCATGATGATTGCAGGCCATGGTGAGTAA

Upstream 100 bases:

>100_bases
GAAAGATGATCGAACCCATATATTCATGTAATCGACCCAGATAATCTGGAAGTTTTATCGGCGCGCGTGATCGCGGTCGC
CCTGCAATAGTTGAAAACAG

Downstream 100 bases:

>100_bases
TACCACCAAGAAGAAAACACAGGTGTGCACCGCGTGAACGACACTTTCGATTATATAATGCGCTTTGCCATCGTGGTTTT
CAGCTATTGCTGTGCGGTTC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSISFDLTIDDTRDQLARHARASLEAKPSLIGMSREEMAAALIAAGVPERQVKMRISQLWHWLYVRGVSDFADMRNISKD
LRAMLAQHFTIARPEVVEEQISQDGTRKWLFRFPPRSAGRPVEIESVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLV
RNLTSEEILAQLLTARDRLGDFPDKDTPDGAMVPAEGRKITNIVMMGMGEPLYNFEEVKKALLIASDGDGLSLSKRRITL
STSGVVPEIYRTGDEIGVMLAISLHAVRDELRDILVPINKKYPLAELIKACREYPGLSNAKRITFEYVMLKDINDSLDDA
KLLVKLLQGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADYVNAAGYASPIRTPRGRDILAACGQLKSESERLRKSERLA
LEAMMIAGHGE

Sequences:

>Translated_411_residues
MSISFDLTIDDTRDQLARHARASLEAKPSLIGMSREEMAAALIAAGVPERQVKMRISQLWHWLYVRGVSDFADMRNISKD
LRAMLAQHFTIARPEVVEEQISQDGTRKWLFRFPPRSAGRPVEIESVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLV
RNLTSEEILAQLLTARDRLGDFPDKDTPDGAMVPAEGRKITNIVMMGMGEPLYNFEEVKKALLIASDGDGLSLSKRRITL
STSGVVPEIYRTGDEIGVMLAISLHAVRDELRDILVPINKKYPLAELIKACREYPGLSNAKRITFEYVMLKDINDSLDDA
KLLVKLLQGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADYVNAAGYASPIRTPRGRDILAACGQLKSESERLRKSERLA
LEAMMIAGHGE
>Mature_410_residues
SISFDLTIDDTRDQLARHARASLEAKPSLIGMSREEMAAALIAAGVPERQVKMRISQLWHWLYVRGVSDFADMRNISKDL
RAMLAQHFTIARPEVVEEQISQDGTRKWLFRFPPRSAGRPVEIESVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVR
NLTSEEILAQLLTARDRLGDFPDKDTPDGAMVPAEGRKITNIVMMGMGEPLYNFEEVKKALLIASDGDGLSLSKRRITLS
TSGVVPEIYRTGDEIGVMLAISLHAVRDELRDILVPINKKYPLAELIKACREYPGLSNAKRITFEYVMLKDINDSLDDAK
LLVKLLQGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADYVNAAGYASPIRTPRGRDILAACGQLKSESERLRKSERLAL
EAMMIAGHGE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=385, Percent_Identity=43.1168831168831, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 45897; Mature: 45765

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISFDLTIDDTRDQLARHARASLEAKPSLIGMSREEMAAALIAAGVPERQVKMRISQLW
CCEEEEEEECCHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
HWLYVRGVSDFADMRNISKDLRAMLAQHFTIARPEVVEEQISQDGTRKWLFRFPPRSAGR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
PVEIESVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTSEEILAQLLTARDRLG
CEEEEEEECCCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCC
DFPDKDTPDGAMVPAEGRKITNIVMMGMGEPLYNFEEVKKALLIASDGDGLSLSKRRITL
CCCCCCCCCCCEECCCCCEEEEEEEECCCCCCCCHHHHHHHEEEECCCCCCEEECEEEEE
STSGVVPEIYRTGDEIGVMLAISLHAVRDELRDILVPINKKYPLAELIKACREYPGLSNA
ECCCCCHHHHHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
KRITFEYVMLKDINDSLDDAKLLVKLLQGIPAKINLIPFNPWPGTNYQCSDWEQIEKFAD
CEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHH
YVNAAGYASPIRTPRGRDILAACGQLKSESERLRKSERLALEAMMIAGHGE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
SISFDLTIDDTRDQLARHARASLEAKPSLIGMSREEMAAALIAAGVPERQVKMRISQLW
CEEEEEEECCHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
HWLYVRGVSDFADMRNISKDLRAMLAQHFTIARPEVVEEQISQDGTRKWLFRFPPRSAGR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
PVEIESVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTSEEILAQLLTARDRLG
CEEEEEEECCCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHHCCHHHHHHHHHHHHHHCC
DFPDKDTPDGAMVPAEGRKITNIVMMGMGEPLYNFEEVKKALLIASDGDGLSLSKRRITL
CCCCCCCCCCCEECCCCCEEEEEEEECCCCCCCCHHHHHHHEEEECCCCCCEEECEEEEE
STSGVVPEIYRTGDEIGVMLAISLHAVRDELRDILVPINKKYPLAELIKACREYPGLSNA
ECCCCCHHHHHCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
KRITFEYVMLKDINDSLDDAKLLVKLLQGIPAKINLIPFNPWPGTNYQCSDWEQIEKFAD
CEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHH
YVNAAGYASPIRTPRGRDILAACGQLKSESERLRKSERLALEAMMIAGHGE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA