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The map label for this gene is rhtB [H]

Identifier: 225851616

GI number: 225851616

Start: 83422

End: 84081

Strand: Direct

Name: rhtB [H]

Synonym: BMEA_A0078

Alternate gene names: 225851616

Gene position: 83422-84081 (Clockwise)

Preceding gene: 225851614

Following gene: 225851625

Centisome position: 3.92

GC content: 56.97

Gene sequence:

>660_bases
ATGCCATTCCTGACAAACCTGACCTTCATTCCCGAATGGACAATCTTTGTCCAGTTCGCGATAGCAACTGCGATCCTTTC
CATCACGCCCGGCCCCGACATGACGCTTTTCGTCGGGCGTGCCCTGTCCGAAGGCAAGGCGGCAGGTTTTGCCTGCATGG
CTGGCGCCAGCACCGGCATTGTCATTCACACCAGCATGGTGGCGCTCGGTCTTTCGGCGCTTATTGTGGCATCGCCTGCG
GCCTTTACGGCGCTGAAAGTGGTGGGGGCGGGCTATCTGGTCTGGCTCGCCGTGCAGGCGATCTGCAAGGGGTCGGCCTT
CTCGCCGGAAAAGAATGGCGGCAAGAAGCATACACTGTTTCAGAACTGGCTGACCGGGCTTGGCATCAATCTTCTCAATC
CGAAGATCATCCTGTTCAATATGACATTCCTGCCGCAGTTCGTCTCCGCGCATGATCCTCATGCCATGGGCAAACTGTTC
TTCCTCGGTCTGTCGTTTATTCCCATGGCGCTGCCCTTCACCATTCCCATGGTGGTGGCGGCAGATCGTTTTGCCGGGCT
TCTGAACAAGAACCCGACTGTGACGCGCATTGTGGACTGGATGTTTGCCGGTGTATTCTCGGCCTTCGCGCTGAAAATCA
TCACCGCACAGGCAAAGTAG

Upstream 100 bases:

>100_bases
TGCAAGCAGGATGGCTGCGACTTATAGAAAAGACGAGAGAAACCAGTGGCGGCGGACCTTGATTCGCGTTAGCGTGCCGC
GAATTTTCGGAGCTTTTCGC

Downstream 100 bases:

>100_bases
AAATCAGTCTGTCGGGCGGGATAAAAGCCGCCCGGCATTGCGGCCTGCAAGCAGCCAATAGACGAAGCCGCCGACAATAC
CGGCCCCTGTCGTTGCCGCG

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MPFLTNLTFIPEWTIFVQFAIATAILSITPGPDMTLFVGRALSEGKAAGFACMAGASTGIVIHTSMVALGLSALIVASPA
AFTALKVVGAGYLVWLAVQAICKGSAFSPEKNGGKKHTLFQNWLTGLGINLLNPKIILFNMTFLPQFVSAHDPHAMGKLF
FLGLSFIPMALPFTIPMVVAADRFAGLLNKNPTVTRIVDWMFAGVFSAFALKIITAQAK

Sequences:

>Translated_219_residues
MPFLTNLTFIPEWTIFVQFAIATAILSITPGPDMTLFVGRALSEGKAAGFACMAGASTGIVIHTSMVALGLSALIVASPA
AFTALKVVGAGYLVWLAVQAICKGSAFSPEKNGGKKHTLFQNWLTGLGINLLNPKIILFNMTFLPQFVSAHDPHAMGKLF
FLGLSFIPMALPFTIPMVVAADRFAGLLNKNPTVTRIVDWMFAGVFSAFALKIITAQAK
>Mature_218_residues
PFLTNLTFIPEWTIFVQFAIATAILSITPGPDMTLFVGRALSEGKAAGFACMAGASTGIVIHTSMVALGLSALIVASPAA
FTALKVVGAGYLVWLAVQAICKGSAFSPEKNGGKKHTLFQNWLTGLGINLLNPKIILFNMTFLPQFVSAHDPHAMGKLFF
LGLSFIPMALPFTIPMVVAADRFAGLLNKNPTVTRIVDWMFAGVFSAFALKIITAQAK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=31.3131313131313, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23322; Mature: 23191

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLTNLTFIPEWTIFVQFAIATAILSITPGPDMTLFVGRALSEGKAAGFACMAGASTGI
CCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHCCCCCCCEEEECCCCCCE
VIHTSMVALGLSALIVASPAAFTALKVVGAGYLVWLAVQAICKGSAFSPEKNGGKKHTLF
EEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QNWLTGLGINLLNPKIILFNMTFLPQFVSAHDPHAMGKLFFLGLSFIPMALPFTIPMVVA
HHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFAGLLNKNPTVTRIVDWMFAGVFSAFALKIITAQAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PFLTNLTFIPEWTIFVQFAIATAILSITPGPDMTLFVGRALSEGKAAGFACMAGASTGI
CCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHCCCCCCCEEEECCCCCCE
VIHTSMVALGLSALIVASPAAFTALKVVGAGYLVWLAVQAICKGSAFSPEKNGGKKHTLF
EEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QNWLTGLGINLLNPKIILFNMTFLPQFVSAHDPHAMGKLFFLGLSFIPMALPFTIPMVVA
HHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFAGLLNKNPTVTRIVDWMFAGVFSAFALKIITAQAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]