Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 225630974

GI number: 225630974

Start: 1383353

End: 1383859

Strand: Reverse

Name: nuoB

Synonym: WRi_012960

Alternate gene names: 225630974

Gene position: 1383859-1383353 (Counterclockwise)

Preceding gene: 225630975

Following gene: 225630973

Centisome position: 95.71

GC content: 41.22

Gene sequence:

>507_bases
ATGACAAATCAAATTCTATCTAATGATGACTGGGGTCGTTATAAAAAAGAAGGGTTTCTTGTCACTAAATTTGGTGATTT
AACAGATTATGTAATGAATTGGGCAAGGTCTGGTTCATTGTGGCCAATGACATTTGGTCTTGCATGTTGCGCAGTTGAGA
TGATGCATACTGCATCCAGCCGTTATGATCTTGATAGATATGGTATAATGTTTCGTGCAAGCCCACGTCAATCAGATGTG
ATGATTGTTGCTGGCACACTAACTAATAAAATGGCAGCAGCATTGCGCAAGGTTTATGATCAAATGGCAGACCCGAAGTA
TGTTATTTCCATGGGAAGTTGTGCAAATGGTGGTGGTTATTACCATTACTCTTATTCAGTAGTCCGAGGCTGTGATAGAA
TTGTGCCAGTTGACGTGTATGTCCCTGGATGCCCACCAACCGCTGAGGCGTTGCTATATGGAATGCTATGCCTACAAAAC
AAAATAAAACGGACTCGGAATGGATAA

Upstream 100 bases:

>100_bases
AAGCTATGGTGGATTTTGGTCTATGATGGTATTTCTTGCAATACTCACTATAGGTTTTATCTATGAATGGTGTAAAGGTG
CATTAGAGTGGGAGTAGTTT

Downstream 100 bases:

>100_bases
AACTGCAAAGTATATACAAAAAAAGACCAAATGCGAGTGCATTCAAAAAGATGATGGCATTATTGTAATATATTCAACTT
TAGATGAAATTGAAAAGCAC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTNQILSNDDWGRYKKEGFLVTKFGDLTDYVMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIMFRASPRQSDV
MIVAGTLTNKMAAALRKVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQN
KIKRTRNG

Sequences:

>Translated_168_residues
MTNQILSNDDWGRYKKEGFLVTKFGDLTDYVMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIMFRASPRQSDV
MIVAGTLTNKMAAALRKVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQN
KIKRTRNG
>Mature_167_residues
TNQILSNDDWGRYKKEGFLVTKFGDLTDYVMNWARSGSLWPMTFGLACCAVEMMHTASSRYDLDRYGIMFRASPRQSDVM
IVAGTLTNKMAAALRKVYDQMADPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNK
IKRTRNG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=79.1946308724832, Blast_Score=256, Evalue=7e-69,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=52.0833333333333, Blast_Score=161, Evalue=2e-41,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=44.3396226415094, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=38.0165289256198, Blast_Score=97, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=74.2857142857143, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=78.4172661870504, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=78.4172661870504, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24651058, Length=161, Percent_Identity=72.0496894409938, Blast_Score=247, Evalue=3e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_WOLPM (Q73G42)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966842.1
- ProteinModelPortal:   Q73G42
- EnsemblBacteria:   EBWOLT00000004092
- GeneID:   2738158
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD1123
- TIGR:   WD_1123
- GeneTree:   EBGT00050000031222
- HOGENOM:   HBG553221
- OMA:   EAKWVIS
- PhylomeDB:   Q73G42
- ProtClustDB:   PRK06411
- BioCyc:   WPIP955:WD_1123-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 18821; Mature: 18690

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQILSNDDWGRYKKEGFLVTKFGDLTDYVMNWARSGSLWPMTFGLACCAVEMMHTASS
CCCCCCCCCCCCCCHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
RYDLDRYGIMFRASPRQSDVMIVAGTLTNKMAAALRKVYDQMADPKYVISMGSCANGGGY
CCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
YHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNKIKRTRNG
EEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNQILSNDDWGRYKKEGFLVTKFGDLTDYVMNWARSGSLWPMTFGLACCAVEMMHTASS
CCCCCCCCCCCCCHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
RYDLDRYGIMFRASPRQSDVMIVAGTLTNKMAAALRKVYDQMADPKYVISMGSCANGGGY
CCCHHHHCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCE
YHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGMLCLQNKIKRTRNG
EEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA