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The map label for this gene is tilS [H]

Identifier: 225630913

GI number: 225630913

Start: 1320367

End: 1321662

Strand: Reverse

Name: tilS [H]

Synonym: WRi_012290

Alternate gene names: 225630913

Gene position: 1321662-1320367 (Counterclockwise)

Preceding gene: 225630914

Following gene: 225630912

Centisome position: 91.41

GC content: 33.8

Gene sequence:

>1296_bases
ATGGAATTAGAGTTATTATTTCAAAATGTGGTTAATAGTTTTGCTTTCTATAACAATCAAGTTGCAGTTGCAGTATCAGG
TGGTGTAGATAGTATAGTCTTACTGCACTTAATGACTAACTGGGCAAAAAAAAACAAGCTTTCACTTCCTACAGCATTAA
CAGTAAATCATGGGTTACGTCCAGAGTCTCAAAAAGAAGCTGATTTTGTTGTAAGTTATGCAAAGGAACTTGGAGCAAAG
GAATCGTTCATATTAAATTGGGAGAAGCAAAATATTAAAGGCAATATTCAGTTACAGGCACGAAAAGCACGGTATAAGTT
ACTAGCAGAGTGGTGTAAAAATAATAATGTTAAATATTTACTTGTTGCTCATCACAAAGATGATCAAGCAGAAACGTTCT
TGTTAAGATTAGAGCGAGGTAGTGGCGTAGATGGATTATCATCAATGGACTACAAGTCTTTCCTAAATGGTATTTATATA
TTTAGGCCATTGTTAAATTTTAGTCGTAGTGAAATAGAAAAGTACGCTAAGCTTCATCGGTTAAAATGGATCGAAGATAG
AAGCAACTATGACTTAAAATACAGGCGAACTTTATACCGTAACTTACTTAAAGCAAGTGATAATCAAGAGATTTTAACAG
AGCGAATATGCCTCACAGCCCTTCATATGAAAAGAGCTGCAAAAGCGTTGATGCACTACACACGCCTTGCGTTTAATGAC
TGCGTTAATGTGCATGATCTTGGTTATATTGAAATTAAACTAAGTGAGTTTTATCAATTGCCAGAGGAAATAGCCTTGAG
GCTTCTTCTTTACTCCATAATGGCAATTGCCAGTAAACATTATAAACCAAGGTACAACAGCCTTATCGTAATATTTAATA
AAATATTGCAAAAGGATAGTAATGTTAATTGTACACTTTCTGGGTGTAAAATAAGAAAACATGGAGAAAATATCTTGATA
ATTAGAGAATCGTCAAAGATACAAGAAATTACCTTAAACCTACCTTTAAATGAACCTACTCAATGGGATAACAGATTTAG
CTGCACAATACTCGGAAATCAAGAGTGTTCAGTTATCATCGCTCCATTAAAAAAAACACAAAAAGTTCCTGAATTTCTGA
AGGATTACAACTGCTGCCCTGAAGTTTTCTATTCTTTGCCTGTAGTGCTAAAAGATGGAAAGGTGCTTGCTTATCCTGAT
GTAAATTATAACGGAAAAAATACCAATGACGATAAGGTTCAATGCATTATTAATAGCACGATAAAACAAAATTTGGTAAG
CTTGATTAGTATTTAG

Upstream 100 bases:

>100_bases
CTTGTCATATTTCGTTGCTTGTTAGCGGGATCTATATATACCACAAATAAGTCGCGGTATGACGTAAGAGATCTGACCTG
TCTGGCCATCAAGAGACATA

Downstream 100 bases:

>100_bases
TTAGAAATAGAAATGAAAAAATTTTTAGAAGGCTTATTGATCTGGTTAGTAATTATTGTTCTTATTTCAGTTGCTTATAT
TCAGTTCAGCGGAAATGTAG

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MELELLFQNVVNSFAFYNNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLPTALTVNHGLRPESQKEADFVVSYAKELGAK
ESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKYLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYI
FRPLLNFSRSEIEKYAKLHRLKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICLTALHMKRAAKALMHYTRLAFND
CVNVHDLGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKDSNVNCTLSGCKIRKHGENILI
IRESSKIQEITLNLPLNEPTQWDNRFSCTILGNQECSVIIAPLKKTQKVPEFLKDYNCCPEVFYSLPVVLKDGKVLAYPD
VNYNGKNTNDDKVQCIINSTIKQNLVSLISI

Sequences:

>Translated_431_residues
MELELLFQNVVNSFAFYNNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLPTALTVNHGLRPESQKEADFVVSYAKELGAK
ESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKYLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYI
FRPLLNFSRSEIEKYAKLHRLKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICLTALHMKRAAKALMHYTRLAFND
CVNVHDLGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKDSNVNCTLSGCKIRKHGENILI
IRESSKIQEITLNLPLNEPTQWDNRFSCTILGNQECSVIIAPLKKTQKVPEFLKDYNCCPEVFYSLPVVLKDGKVLAYPD
VNYNGKNTNDDKVQCIINSTIKQNLVSLISI
>Mature_431_residues
MELELLFQNVVNSFAFYNNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLPTALTVNHGLRPESQKEADFVVSYAKELGAK
ESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKYLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYI
FRPLLNFSRSEIEKYAKLHRLKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICLTALHMKRAAKALMHYTRLAFND
CVNVHDLGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKDSNVNCTLSGCKIRKHGENILI
IRESSKIQEITLNLPLNEPTQWDNRFSCTILGNQECSVIIAPLKKTQKVPEFLKDYNCCPEVFYSLPVVLKDGKVLAYPD
VNYNGKNTNDDKVQCIINSTIKQNLVSLISI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=194, Percent_Identity=38.1443298969072, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 49680; Mature: 49680

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELELLFQNVVNSFAFYNNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLPTALTVNHGLR
CCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PESQKEADFVVSYAKELGAKESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKYL
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCEEEE
LVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIFRPLLNFSRSEIEKYAKLHR
EEEECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICLTALHMKRAAKALMHYTRLAFND
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVNVHDLGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKDS
CCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
NVNCTLSGCKIRKHGENILIIRESSKIQEITLNLPLNEPTQWDNRFSCTILGNQECSVII
CCEEEEECCEEEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEEE
APLKKTQKVPEFLKDYNCCPEVFYSLPVVLKDGKVLAYPDVNYNGKNTNDDKVQCIINST
ECHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCEEEEEHHH
IKQNLVSLISI
HHHHHHHHHCC
>Mature Secondary Structure
MELELLFQNVVNSFAFYNNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLPTALTVNHGLR
CCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
PESQKEADFVVSYAKELGAKESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKYL
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCEEEE
LVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIFRPLLNFSRSEIEKYAKLHR
EEEECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICLTALHMKRAAKALMHYTRLAFND
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVNVHDLGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKDS
CCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
NVNCTLSGCKIRKHGENILIIRESSKIQEITLNLPLNEPTQWDNRFSCTILGNQECSVII
CCEEEEECCEEEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEEE
APLKKTQKVPEFLKDYNCCPEVFYSLPVVLKDGKVLAYPDVNYNGKNTNDDKVQCIINST
ECHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCEEEEEHHH
IKQNLVSLISI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA