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The map label for this gene is rsmE [H]

Identifier: 225630878

GI number: 225630878

Start: 1281077

End: 1281763

Strand: Reverse

Name: rsmE [H]

Synonym: WRi_011910

Alternate gene names: 225630878

Gene position: 1281763-1281077 (Counterclockwise)

Preceding gene: 225630879

Following gene: 225630877

Centisome position: 88.65

GC content: 35.95

Gene sequence:

>687_bases
ATGGGAAAAATTAGGCTCTATGTTGAAGAAGCTTTATCACAAGGCGTGAGTTTAGCACTTAATCCACAACAAAGTCACTA
TATTTGCAATGTAATGCGGCTTAAGAAGTATGATAATCTCTCTCTTTTTAATGGAAAAGATGGAGAATGGTTAGGAGAAG
TAGTTAATATATCGCGTAAATTGACAGAAGTTACACCCAAAGAATGCACTAAACAACAGCAATATGAGGAAAATTTATAC
CTGTATTGTGCCATGGTAAAAAGTGCTGCTTTGAACAACATAGTAAGACAGGCAACTGAAATGGGAGTAATCTGCATTCA
ATTTATTTCAACGGAACGTACAGTGGTAAAAAATATTAACCTAAGTAGAGCAAAATTACAAGCAATCGAAGCAGCGGAAC
AATCAGGTAGGATAAGTATACCAGAGATTTTGCCTCCTATTAACTTTTGTGAGTTACCTGATTCCCAGAGTAAAAATTTT
GTTTTGTGTGATGAAACAGGTAAAGCTGACAAAGTGCTGAAAGGCAAAAAAAATGTTGCTATTATTGTTGGCCCTGAAGG
TGGTTTTTCATCTTATGAACTTGATCTTGCCGATAAGTTTTGTCAAAAATTGAGTCTAGGAAAAAGAATTTTAAGGGTCG
ATACTGCTGTAGTTGCTGCACTCACTTTCACCAATTGGTGTAGCTAG

Upstream 100 bases:

>100_bases
GACTCTTTCGTCTAGTGGTTAGGACACCACCCTTTCACGGTGGTAACACGGGTTCAAATCCCGTAAGAGTCACTCAAGTA
GAATATAATTAAAGTTTTGA

Downstream 100 bases:

>100_bases
ATTTTTAAATATGACTAAGGTTTGGGTCATAAAGAAATCTGGTTCAGTGTTGCGCGCACAAAAACCCTGTAGACTTTCTA
CGAAGCAGTCTGTTGTTAAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MGKIRLYVEEALSQGVSLALNPQQSHYICNVMRLKKYDNLSLFNGKDGEWLGEVVNISRKLTEVTPKECTKQQQYEENLY
LYCAMVKSAALNNIVRQATEMGVICIQFISTERTVVKNINLSRAKLQAIEAAEQSGRISIPEILPPINFCELPDSQSKNF
VLCDETGKADKVLKGKKNVAIIVGPEGGFSSYELDLADKFCQKLSLGKRILRVDTAVVAALTFTNWCS

Sequences:

>Translated_228_residues
MGKIRLYVEEALSQGVSLALNPQQSHYICNVMRLKKYDNLSLFNGKDGEWLGEVVNISRKLTEVTPKECTKQQQYEENLY
LYCAMVKSAALNNIVRQATEMGVICIQFISTERTVVKNINLSRAKLQAIEAAEQSGRISIPEILPPINFCELPDSQSKNF
VLCDETGKADKVLKGKKNVAIIVGPEGGFSSYELDLADKFCQKLSLGKRILRVDTAVVAALTFTNWCS
>Mature_227_residues
GKIRLYVEEALSQGVSLALNPQQSHYICNVMRLKKYDNLSLFNGKDGEWLGEVVNISRKLTEVTPKECTKQQQYEENLYL
YCAMVKSAALNNIVRQATEMGVICIQFISTERTVVKNINLSRAKLQAIEAAEQSGRISIPEILPPINFCELPDSQSKNFV
LCDETGKADKVLKGKKNVAIIVGPEGGFSSYELDLADKFCQKLSLGKRILRVDTAVVAALTFTNWCS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=229, Percent_Identity=26.6375545851528, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25416; Mature: 25285

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIRLYVEEALSQGVSLALNPQQSHYICNVMRLKKYDNLSLFNGKDGEWLGEVVNISRK
CCEEEEHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
LTEVTPKECTKQQQYEENLYLYCAMVKSAALNNIVRQATEMGVICIQFISTERTVVKNIN
HHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCC
LSRAKLQAIEAAEQSGRISIPEILPPINFCELPDSQSKNFVLCDETGKADKVLKGKKNVA
CHHHHHHHHHHHHHCCCEECHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
IIVGPEGGFSSYELDLADKFCQKLSLGKRILRVDTAVVAALTFTNWCS
EEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKIRLYVEEALSQGVSLALNPQQSHYICNVMRLKKYDNLSLFNGKDGEWLGEVVNISRK
CEEEEHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
LTEVTPKECTKQQQYEENLYLYCAMVKSAALNNIVRQATEMGVICIQFISTERTVVKNIN
HHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCC
LSRAKLQAIEAAEQSGRISIPEILPPINFCELPDSQSKNFVLCDETGKADKVLKGKKNVA
CHHHHHHHHHHHHHCCCEECHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
IIVGPEGGFSSYELDLADKFCQKLSLGKRILRVDTAVVAALTFTNWCS
EEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]