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The map label for this gene is hslU [H]

Identifier: 225630856

GI number: 225630856

Start: 1252022

End: 1253512

Strand: Direct

Name: hslU [H]

Synonym: WRi_011620

Alternate gene names: 225630856

Gene position: 1252022-1253512 (Clockwise)

Preceding gene: 225630855

Following gene: 225630860

Centisome position: 86.59

GC content: 37.09

Gene sequence:

>1491_bases
ATGTCTTCCAAGCAAAAAACTTTATGCACCAATTTTTCCAATCAGCCCATAACCCTTTCAGAAGGGCGGTCTTGTAGTAG
TGACACCTACGATGAAAAAGATAACCTCTATAAAGATACTAAGAGTGGTTTTGATTCAAATGACAGCGATGTTCAAGTTA
ATGACAGCACTCAAGTTTTGTTAGATGACCTGCCACCACAGAAGATAGTTAAAGAATTGGACAGATTCATAATCGGACAG
GATGATGCAAAGCGTGCTGTTGCTATTGCGCTCAGAAATCGTTGGCGTCGCAATCAGGTTCCATTTCCATTGCGTGATGA
AATCATACCTAAGAATATATTGATGATCGGTCATACAGGGGTTGGTAAAACTGAAATAGCTCGTCGCTTAGCAAAACTTG
CCGGTGCACCGTTCATAAAAATTGAGGCAACGAAGTTTACTGAAATAGGGTACGTTGGACGTGACGTTGATTCGATAATA
CGCGATTTAGTTGATGCAGCAATAGTTTTAGTTAAAGAGAAAGCCCGTAAAGCCTTAGCTAAAAAGGCTTTAATTTTAGC
TGAGAAAACAATAGTAAACTCTATGGTAGGCGAAAATGCAACCGAAGAGAGTAAAAAAATTTTTAGAGAAAGGTTGAGGA
ATAAAGAGTTTGAGGACGGAGAAGTTTCTATTAACGTCAGAGAGAGCAAGAGTATGTTACCTACTTTCGATATACCAGGC
ATGCCAGGTGGGCAAGTTGGTGTGATGAATGTAACAGAAATAATGGGCAAGATGTTTAACGGGAGCAAAAAAACAAAAAC
TATTACGGTGAAGGTAAAAGAAGCGCGTGAAATATTAATTAATGAAGAAAGTGAAAGGCTAATGGATGAAGATAAGATAA
TCAAAGAAGCCATTGATCTTGTTAGTAATGACGGTATAGTATTTTTGGATGAAATAGACAAAATTGCAGCGCGTACAGAA
GTAAAAGGTGAAGTAAACAGAGAAGGAGTGCAACGCGATCTGTTACCATTACTTGAAGGAACAACTGTTACAACTAAGTA
TGGCCATGTAAAAACAGACTATATATTATTTATTGCATCTGGTGCTTTTCATCAGTCTAAACCATCTGACCTCTTACCGG
AATTGCAGGGCAGATTACCGATCAGGGTAGAACTTAAGGCACTTACTCAAGAGGATTTAATAAGAATATTAAAGGAACCA
GAATCTAGTTTGTTAAAGCAGTACATAGCTTTAATGAAAACAGAAAATGTGACACTTGAGTTCACTGATGATGGTATAAA
AACCATAGCTGAAATAGCGTTTACAGTTAATAGGCAAGTGGAAAATATAGGTGCAAGAAGGCTTCACACTGTCATGGAGA
AGCTTTTAGATGAAATAAGTTTCATTGCTTCTGAGAAAAATAGCGAAAAATTCATTATAGACAGCAAGTATGTAAAAGAT
AAACTTGAGTCAATTTCGAAGCAGCTTGATTTATCTAAGTTTATACTTTAG

Upstream 100 bases:

>100_bases
ATATCAATAGAGGAAATTGCGAAAAAGGCTATGAAGATAGCTGGCGATATATGTGTTTATACAAATCATAATGTAGTTAT
TGAAAAGATAGAGGAGTGAT

Downstream 100 bases:

>100_bases
GAAAAACGAAAAGCCTACTTTAAGTAGTATAGAGAGTGTTCAGAAGATTAGCCAGTAGACCTAAAATTTCGTATCCGTTC
AGGAGAGTGGTTTAAGAAAC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSSKQKTLCTNFSNQPITLSEGRSCSSDTYDEKDNLYKDTKSGFDSNDSDVQVNDSTQVLLDDLPPQKIVKELDRFIIGQ
DDAKRAVAIALRNRWRRNQVPFPLRDEIIPKNILMIGHTGVGKTEIARRLAKLAGAPFIKIEATKFTEIGYVGRDVDSII
RDLVDAAIVLVKEKARKALAKKALILAEKTIVNSMVGENATEESKKIFRERLRNKEFEDGEVSINVRESKSMLPTFDIPG
MPGGQVGVMNVTEIMGKMFNGSKKTKTITVKVKEAREILINEESERLMDEDKIIKEAIDLVSNDGIVFLDEIDKIAARTE
VKGEVNREGVQRDLLPLLEGTTVTTKYGHVKTDYILFIASGAFHQSKPSDLLPELQGRLPIRVELKALTQEDLIRILKEP
ESSLLKQYIALMKTENVTLEFTDDGIKTIAEIAFTVNRQVENIGARRLHTVMEKLLDEISFIASEKNSEKFIIDSKYVKD
KLESISKQLDLSKFIL

Sequences:

>Translated_496_residues
MSSKQKTLCTNFSNQPITLSEGRSCSSDTYDEKDNLYKDTKSGFDSNDSDVQVNDSTQVLLDDLPPQKIVKELDRFIIGQ
DDAKRAVAIALRNRWRRNQVPFPLRDEIIPKNILMIGHTGVGKTEIARRLAKLAGAPFIKIEATKFTEIGYVGRDVDSII
RDLVDAAIVLVKEKARKALAKKALILAEKTIVNSMVGENATEESKKIFRERLRNKEFEDGEVSINVRESKSMLPTFDIPG
MPGGQVGVMNVTEIMGKMFNGSKKTKTITVKVKEAREILINEESERLMDEDKIIKEAIDLVSNDGIVFLDEIDKIAARTE
VKGEVNREGVQRDLLPLLEGTTVTTKYGHVKTDYILFIASGAFHQSKPSDLLPELQGRLPIRVELKALTQEDLIRILKEP
ESSLLKQYIALMKTENVTLEFTDDGIKTIAEIAFTVNRQVENIGARRLHTVMEKLLDEISFIASEKNSEKFIIDSKYVKD
KLESISKQLDLSKFIL
>Mature_495_residues
SSKQKTLCTNFSNQPITLSEGRSCSSDTYDEKDNLYKDTKSGFDSNDSDVQVNDSTQVLLDDLPPQKIVKELDRFIIGQD
DAKRAVAIALRNRWRRNQVPFPLRDEIIPKNILMIGHTGVGKTEIARRLAKLAGAPFIKIEATKFTEIGYVGRDVDSIIR
DLVDAAIVLVKEKARKALAKKALILAEKTIVNSMVGENATEESKKIFRERLRNKEFEDGEVSINVRESKSMLPTFDIPGM
PGGQVGVMNVTEIMGKMFNGSKKTKTITVKVKEAREILINEESERLMDEDKIIKEAIDLVSNDGIVFLDEIDKIAARTEV
KGEVNREGVQRDLLPLLEGTTVTTKYGHVKTDYILFIASGAFHQSKPSDLLPELQGRLPIRVELKALTQEDLIRILKEPE
SSLLKQYIALMKTENVTLEFTDDGIKTIAEIAFTVNRQVENIGARRLHTVMEKLLDEISFIASEKNSEKFIIDSKYVKDK
LESISKQLDLSKFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=91, Percent_Identity=39.5604395604396, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790366, Length=450, Percent_Identity=53.1111111111111, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1786642, Length=230, Percent_Identity=33.4782608695652, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=142, Percent_Identity=34.5070422535211, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648291, Length=242, Percent_Identity=30.5785123966942, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648289, Length=242, Percent_Identity=30.5785123966942, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 55819; Mature: 55688

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00501 SPASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKQKTLCTNFSNQPITLSEGRSCSSDTYDEKDNLYKDTKSGFDSNDSDVQVNDSTQVL
CCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEE
LDDLPPQKIVKELDRFIIGQDDAKRAVAIALRNRWRRNQVPFPLRDEIIPKNILMIGHTG
ECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCC
VGKTEIARRLAKLAGAPFIKIEATKFTEIGYVGRDVDSIIRDLVDAAIVLVKEKARKALA
CCHHHHHHHHHHHHCCCEEEEEECEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KKALILAEKTIVNSMVGENATEESKKIFRERLRNKEFEDGEVSINVRESKSMLPTFDIPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
MPGGQVGVMNVTEIMGKMFNGSKKTKTITVKVKEAREILINEESERLMDEDKIIKEAIDL
CCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSNDGIVFLDEIDKIAARTEVKGEVNREGVQRDLLPLLEGTTVTTKYGHVKTDYILFIAS
HCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEC
GAFHQSKPSDLLPELQGRLPIRVELKALTQEDLIRILKEPESSLLKQYIALMKTENVTLE
CCCCCCCCHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
FTDDGIKTIAEIAFTVNRQVENIGARRLHTVMEKLLDEISFIASEKNSEKFIIDSKYVKD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHH
KLESISKQLDLSKFIL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSKQKTLCTNFSNQPITLSEGRSCSSDTYDEKDNLYKDTKSGFDSNDSDVQVNDSTQVL
CCCCCCHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEE
LDDLPPQKIVKELDRFIIGQDDAKRAVAIALRNRWRRNQVPFPLRDEIIPKNILMIGHTG
ECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCC
VGKTEIARRLAKLAGAPFIKIEATKFTEIGYVGRDVDSIIRDLVDAAIVLVKEKARKALA
CCHHHHHHHHHHHHCCCEEEEEECEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KKALILAEKTIVNSMVGENATEESKKIFRERLRNKEFEDGEVSINVRESKSMLPTFDIPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
MPGGQVGVMNVTEIMGKMFNGSKKTKTITVKVKEAREILINEESERLMDEDKIIKEAIDL
CCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSNDGIVFLDEIDKIAARTEVKGEVNREGVQRDLLPLLEGTTVTTKYGHVKTDYILFIAS
HCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEC
GAFHQSKPSDLLPELQGRLPIRVELKALTQEDLIRILKEPESSLLKQYIALMKTENVTLE
CCCCCCCCHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
FTDDGIKTIAEIAFTVNRQVENIGARRLHTVMEKLLDEISFIASEKNSEKFIIDSKYVKD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHH
KLESISKQLDLSKFIL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA