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The map label for this gene is hslV

Identifier: 225630855

GI number: 225630855

Start: 1251466

End: 1252020

Strand: Direct

Name: hslV

Synonym: WRi_011610

Alternate gene names: 225630855

Gene position: 1251466-1252020 (Clockwise)

Preceding gene: 225630853

Following gene: 225630856

Centisome position: 86.55

GC content: 36.76

Gene sequence:

>555_bases
ATGATTCAACACGATAACAATAAAATGTATGGCACTACTATATTATCAATTAGAAAAGATAAAAGTGTAGTAGTAATAGG
TGACGGACAAGTTTCACTGGGCCACACTGTTATAAAATCTGGAGCAAAAAAAGTTAGGCGTCTTTCTGGTGATTCCGTAA
TTGCTGGATTTGCCGGGGCAACAGCCGACGCATTTACTCTTTTTGAAAGATTAGAATCTAAACTTGACAAGCACCCAGGG
CAATTAATGAGAGCATGTGTTGAACTTGCAAAAGACTGGAGAATGGATAAATATCTGAGGAAATTAGAAGCTATGATGAT
CGTTGCAGATAAATCTATTTCATTAGTAATTACGGGAACAGGTGATGTTCTTGAACCTGAAGATGGAGTTGCAGCTATTG
GCTCTGGGGGAAATTTTGCTCTATCTGCAGCAAGAGCTTTAATTGACATTAAAGGAATATCAATAGAGGAAATTGCGAAA
AAGGCTATGAAGATAGCTGGCGATATATGTGTTTATACAAATCATAATGTAGTTATTGAAAAGATAGAGGAGTGA

Upstream 100 bases:

>100_bases
TATAAGGAAGGTATTATATTATAAGCTATAGTTGAACATATTTAGTAAATTTGACTAAAGATTAAAGTATAATGAGATTC
TTATTTAAAGTGATAGAAAA

Downstream 100 bases:

>100_bases
TATGTCTTCCAAGCAAAAAACTTTATGCACCAATTTTTCCAATCAGCCCATAACCCTTTCAGAAGGGCGGTCTTGTAGTA
GTGACACCTACGATGAAAAA

Product: ATP-dependent protease peptidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MIQHDNNKMYGTTILSIRKDKSVVVIGDGQVSLGHTVIKSGAKKVRRLSGDSVIAGFAGATADAFTLFERLESKLDKHPG
QLMRACVELAKDWRMDKYLRKLEAMMIVADKSISLVITGTGDVLEPEDGVAAIGSGGNFALSAARALIDIKGISIEEIAK
KAMKIAGDICVYTNHNVVIEKIEE

Sequences:

>Translated_184_residues
MIQHDNNKMYGTTILSIRKDKSVVVIGDGQVSLGHTVIKSGAKKVRRLSGDSVIAGFAGATADAFTLFERLESKLDKHPG
QLMRACVELAKDWRMDKYLRKLEAMMIVADKSISLVITGTGDVLEPEDGVAAIGSGGNFALSAARALIDIKGISIEEIAK
KAMKIAGDICVYTNHNVVIEKIEE
>Mature_184_residues
MIQHDNNKMYGTTILSIRKDKSVVVIGDGQVSLGHTVIKSGAKKVRRLSGDSVIAGFAGATADAFTLFERLESKLDKHPG
QLMRACVELAKDWRMDKYLRKLEAMMIVADKSISLVITGTGDVLEPEDGVAAIGSGGNFALSAARALIDIKGISIEEIAK
KAMKIAGDICVYTNHNVVIEKIEE

Specific function: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery

COG id: COG5405

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), peptidase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family. HslV subfamily

Homologues:

Organism=Escherichia coli, GI1790367, Length=171, Percent_Identity=60.8187134502924, Blast_Score=216, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLV_WOLPM (P61479)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966901.1
- ProteinModelPortal:   P61479
- SMR:   P61479
- MEROPS:   T01.006
- EnsemblBacteria:   EBWOLT00000003840
- GeneID:   2738477
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD1189
- TIGR:   WD_1189
- GeneTree:   EBGT00050000031078
- HOGENOM:   HBG288822
- OMA:   AADICVY
- PhylomeDB:   P61479
- ProtClustDB:   PRK05456
- BioCyc:   WPIP955:WD_1189-MONOMER
- HAMAP:   MF_00248
- InterPro:   IPR022281
- InterPro:   IPR001353
- TIGRFAMs:   TIGR03692

Pfam domain/function: PF00227 Proteasome

EC number: 3.4.25.- [C]

Molecular weight: Translated: 19845; Mature: 19845

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: ACT_SITE 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQHDNNKMYGTTILSIRKDKSVVVIGDGQVSLGHTVIKSGAKKVRRLSGDSVIAGFAGA
CCCCCCCEEEEEEEEEEECCCEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCC
TADAFTLFERLESKLDKHPGQLMRACVELAKDWRMDKYLRKLEAMMIVADKSISLVITGT
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCEEEEEECC
GDVLEPEDGVAAIGSGGNFALSAARALIDIKGISIEEIAKKAMKIAGDICVYTNHNVVIE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCEEEE
KIEE
EECC
>Mature Secondary Structure
MIQHDNNKMYGTTILSIRKDKSVVVIGDGQVSLGHTVIKSGAKKVRRLSGDSVIAGFAGA
CCCCCCCEEEEEEEEEEECCCEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCC
TADAFTLFERLESKLDKHPGQLMRACVELAKDWRMDKYLRKLEAMMIVADKSISLVITGT
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCEEEEEECC
GDVLEPEDGVAAIGSGGNFALSAARALIDIKGISIEEIAKKAMKIAGDICVYTNHNVVIE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCEEEE
KIEE
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA