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The map label for this gene is virB4 [H]

Identifier: 225630842

GI number: 225630842

Start: 1235454

End: 1237808

Strand: Direct

Name: virB4 [H]

Synonym: WRi_011480

Alternate gene names: 225630842

Gene position: 1235454-1237808 (Clockwise)

Preceding gene: 225630841

Following gene: 225630843

Centisome position: 85.45

GC content: 31.8

Gene sequence:

>2355_bases
ATGCAGTTAAAAGTACCAACAGTTAACTCTAAGCTTAAAAAAAAGAACAACTTAGCATTACTAGAAAGCATTGATTTAGA
TTTTGTTCCTTATGCTTGTCATTACGATGAAGAAACCATACTAACAAAACAGGGAGAATTGTTAAAAATAATCAAGTTAG
AAGATTATTCTTCTGTTGATAACTACAGCGACCTCAGAACTGAAATTAGAAAAAGCATATCAAAAAATATTAATAGCCTG
TATTTTACCGTTTGGATTCATACCGTTAGAAAGCGCAATAAACTGAGCTTAAAATGGAATAAAACTGAAGACTTCTCTGA
CAACCTACATTCAACGTGGTTTAATAAACTAACTGATAGTAAATTACAGTATATAAATGAACTATACATTGTAATATTGT
TTAGCGATTTTGGCAAGCATACTAATAATTCGTTTTTTTTCAATAGAATAAAGAATAAGCATAAGTTGTCTTTACAAGAA
AGCCATCAGGAGTTGCAAAAGATAACTGACGTAATTCAAAATGATTTAGGACCTTTTGGAGCTAAAAAACTGAGTCTAAG
GTTTAGTGAAGGTAAAATATATTCTGAAATGATAGAATTTCTCCACTATATTGTTACATTAACGCACAAAGATTATCCAA
TACATGAAAGGGACCTGTCGCAATATATTAGAAATTTTAAAATAGCTTTTGGTTTCAATACGTTTCAAACAATATTTGAA
AATCAACAGAAATTTGGCTCTATTTTCAGCATAAAAGAATACCGGGAAATCTCTTTAGGTAATATAGATAGATGCTTGCA
GCTTGAATCTGAATTTATCATTACAGAAATAATGATATTTACTAGTAATAACAAAGCAATAAAAGAATTTAAAAAACAAA
TCAACATGCTGCAAATTAGTGAGGATAATACCTTACTTAAAAGCTCAGGAATTGAAGAAATAATAGAGCTTGAAAAGACC
TCTAGTATAGACTTCTGCCAACAGAAAATTATTTTTACAATATTTGCAGATGATAGGAATAAGTTAGCTGAAAGTATTAG
TGATTTATCTTCTATAATGTCATTAGTTGGCTTCATGATGTTTAGAACTGATTTACATATGGAAAGTCATTTTTGGGCAC
AACTTCCAGGCAATTTTGCTTTTATTACGCAACCGAAAAATATCTTAACAAAATACGCTTGCAGCTTTGCTATGCTGCAC
GATTTTACGTCAGGTACATTAAAAGGAAGAAGGTGGAAAGAAGCGGTAATGATTTTTTTCTCAAAAAAAGGAAATCCTTA
CTTCTTTAATTTTCATGGAAAAAGAAATAATGGCCATACTACGATACTCGGAGCTCCAAATTCAGGCAGAACTTCACTCA
TTAATTTTCTATTATCAGAATCAAGAAAGTTTAACCCAAGAATTGTCATATTGGATAACACTGGAAAATCAATAATTTTT
ACTAAAGCAGTGAGTGGTAAATATTACATAATCGATCCAAAATATAAGGACAAAAGCCTAAAATTTAATCCACTAAACAT
TGAAGATAGTGCTTCCAATCGCAATATGTTGGTTGAACTGATCAGAAGAATGGTAGCCGATACGAGCTTGGTGGACGTAG
AAGAAAAGATAAGAAAAATTGTGGATTCTATATTTGCTATACCCAAGGAATCACGTTCTATTTCACAAATTTCTGAGGTG
CTCTTGCTCCTTGGGGGTAAGATAAGTAGATGGTGTGACGATGGTGACTTTGCTTACTTATTTCAAGATGGTGATGAATC
AGATATTGACTGGGAAACAAAAATTATATCTCTCAACACCGCAAACCTTACAAAACAAAAGGAATGTATGTCCGTGATAC
TTTACTATTTTTTATATTCTTTTGAGGCAAAATGTGATGGCTCGCCTGCAATACTTGTTTTGGATGAAGCATGGGAGATA
AGTAATATTTTTCCTACAGAGAAAGAGTTTGATAACTGGATGCAAAGAATGACGGAACTAAATGTTGTAGTAATCCTAAG
TACTGAGAATTTAAATCTCGCATTTGCTAGCAAATTTACTCAATATTTAGATAAACATGTTGATACAAGAATCTTGATGC
CGAACGTCAATGCAAATAGATTATACATGAAGGCATTTTCTCTATCGAAAGAGGAGCTGAATATAATTCTGCAGACGCCA
ACACAGGAAGGTTTATTTTTGATAAAACAGTACAAAGGATTAGTAACCTTAAATCTGGATTTGAAGGATATGCAAGAAAT
ACATGTACTTTCAGCTAACAAAGAGACTATAAAGTATATGTATGAGGCAATAAAAGAAAAAGGAGAAGAAGTTAATGAGT
GGTTACCGGTGTTTTATGAGAAGTGTAAAGCTTAA

Upstream 100 bases:

>100_bases
ACTTTGCAAAAGATATAAAATTACAATCCGATAGCGAAAAAAAAGATAAAAATTAAGATAGTATTTTTATCTTTTTTAAT
TATCTTTATTGAATACGCAT

Downstream 100 bases:

>100_bases
TTTCTTATTGATATTCTTATTGATATTTGGTCTTTCATATCAAGGGTCTGCTGATTGCCCATGGAATGCGAAAAGTAATA
CTGAAGTTTCTTCAACATTT

Product: Type IV secretion system protein VirB4, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 784; Mature: 784

Protein sequence:

>784_residues
MQLKVPTVNSKLKKKNNLALLESIDLDFVPYACHYDEETILTKQGELLKIIKLEDYSSVDNYSDLRTEIRKSISKNINSL
YFTVWIHTVRKRNKLSLKWNKTEDFSDNLHSTWFNKLTDSKLQYINELYIVILFSDFGKHTNNSFFFNRIKNKHKLSLQE
SHQELQKITDVIQNDLGPFGAKKLSLRFSEGKIYSEMIEFLHYIVTLTHKDYPIHERDLSQYIRNFKIAFGFNTFQTIFE
NQQKFGSIFSIKEYREISLGNIDRCLQLESEFIITEIMIFTSNNKAIKEFKKQINMLQISEDNTLLKSSGIEEIIELEKT
SSIDFCQQKIIFTIFADDRNKLAESISDLSSIMSLVGFMMFRTDLHMESHFWAQLPGNFAFITQPKNILTKYACSFAMLH
DFTSGTLKGRRWKEAVMIFFSKKGNPYFFNFHGKRNNGHTTILGAPNSGRTSLINFLLSESRKFNPRIVILDNTGKSIIF
TKAVSGKYYIIDPKYKDKSLKFNPLNIEDSASNRNMLVELIRRMVADTSLVDVEEKIRKIVDSIFAIPKESRSISQISEV
LLLLGGKISRWCDDGDFAYLFQDGDESDIDWETKIISLNTANLTKQKECMSVILYYFLYSFEAKCDGSPAILVLDEAWEI
SNIFPTEKEFDNWMQRMTELNVVVILSTENLNLAFASKFTQYLDKHVDTRILMPNVNANRLYMKAFSLSKEELNIILQTP
TQEGLFLIKQYKGLVTLNLDLKDMQEIHVLSANKETIKYMYEAIKEKGEEVNEWLPVFYEKCKA

Sequences:

>Translated_784_residues
MQLKVPTVNSKLKKKNNLALLESIDLDFVPYACHYDEETILTKQGELLKIIKLEDYSSVDNYSDLRTEIRKSISKNINSL
YFTVWIHTVRKRNKLSLKWNKTEDFSDNLHSTWFNKLTDSKLQYINELYIVILFSDFGKHTNNSFFFNRIKNKHKLSLQE
SHQELQKITDVIQNDLGPFGAKKLSLRFSEGKIYSEMIEFLHYIVTLTHKDYPIHERDLSQYIRNFKIAFGFNTFQTIFE
NQQKFGSIFSIKEYREISLGNIDRCLQLESEFIITEIMIFTSNNKAIKEFKKQINMLQISEDNTLLKSSGIEEIIELEKT
SSIDFCQQKIIFTIFADDRNKLAESISDLSSIMSLVGFMMFRTDLHMESHFWAQLPGNFAFITQPKNILTKYACSFAMLH
DFTSGTLKGRRWKEAVMIFFSKKGNPYFFNFHGKRNNGHTTILGAPNSGRTSLINFLLSESRKFNPRIVILDNTGKSIIF
TKAVSGKYYIIDPKYKDKSLKFNPLNIEDSASNRNMLVELIRRMVADTSLVDVEEKIRKIVDSIFAIPKESRSISQISEV
LLLLGGKISRWCDDGDFAYLFQDGDESDIDWETKIISLNTANLTKQKECMSVILYYFLYSFEAKCDGSPAILVLDEAWEI
SNIFPTEKEFDNWMQRMTELNVVVILSTENLNLAFASKFTQYLDKHVDTRILMPNVNANRLYMKAFSLSKEELNIILQTP
TQEGLFLIKQYKGLVTLNLDLKDMQEIHVLSANKETIKYMYEAIKEKGEEVNEWLPVFYEKCKA
>Mature_784_residues
MQLKVPTVNSKLKKKNNLALLESIDLDFVPYACHYDEETILTKQGELLKIIKLEDYSSVDNYSDLRTEIRKSISKNINSL
YFTVWIHTVRKRNKLSLKWNKTEDFSDNLHSTWFNKLTDSKLQYINELYIVILFSDFGKHTNNSFFFNRIKNKHKLSLQE
SHQELQKITDVIQNDLGPFGAKKLSLRFSEGKIYSEMIEFLHYIVTLTHKDYPIHERDLSQYIRNFKIAFGFNTFQTIFE
NQQKFGSIFSIKEYREISLGNIDRCLQLESEFIITEIMIFTSNNKAIKEFKKQINMLQISEDNTLLKSSGIEEIIELEKT
SSIDFCQQKIIFTIFADDRNKLAESISDLSSIMSLVGFMMFRTDLHMESHFWAQLPGNFAFITQPKNILTKYACSFAMLH
DFTSGTLKGRRWKEAVMIFFSKKGNPYFFNFHGKRNNGHTTILGAPNSGRTSLINFLLSESRKFNPRIVILDNTGKSIIF
TKAVSGKYYIIDPKYKDKSLKFNPLNIEDSASNRNMLVELIRRMVADTSLVDVEEKIRKIVDSIFAIPKESRSISQISEV
LLLLGGKISRWCDDGDFAYLFQDGDESDIDWETKIISLNTANLTKQKECMSVILYYFLYSFEAKCDGSPAILVLDEAWEI
SNIFPTEKEFDNWMQRMTELNVVVILSTENLNLAFASKFTQYLDKHVDTRILMPNVNANRLYMKAFSLSKEELNIILQTP
TQEGLFLIKQYKGLVTLNLDLKDMQEIHVLSANKETIKYMYEAIKEKGEEVNEWLPVFYEKCKA

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 91246; Mature: 91246

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKVPTVNSKLKKKNNLALLESIDLDFVPYACHYDEETILTKQGELLKIIKLEDYSSVD
CEEECCCCCHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCEEEEEEECCCCCCC
NYSDLRTEIRKSISKNINSLYFTVWIHTVRKRNKLSLKWNKTEDFSDNLHSTWFNKLTDS
CHHHHHHHHHHHHHCCHHHEEEEEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
KLQYINELYIVILFSDFGKHTNNSFFFNRIKNKHKLSLQESHQELQKITDVIQNDLGPFG
HHHHHHHEEEEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AKKLSLRFSEGKIYSEMIEFLHYIVTLTHKDYPIHERDLSQYIRNFKIAFGFNTFQTIFE
CCEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHEEEEECCHHHHHHHH
NQQKFGSIFSIKEYREISLGNIDRCLQLESEFIITEIMIFTSNNKAIKEFKKQINMLQIS
CHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHCCEEEEC
EDNTLLKSSGIEEIIELEKTSSIDFCQQKIIFTIFADDRNKLAESISDLSSIMSLVGFMM
CCCCCHHHCCHHHHHHHHCCCCHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FRTDLHMESHFWAQLPGNFAFITQPKNILTKYACSFAMLHDFTSGTLKGRRWKEAVMIFF
HHHHCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEE
SKKGNPYFFNFHGKRNNGHTTILGAPNSGRTSLINFLLSESRKFNPRIVILDNTGKSIIF
ECCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEE
TKAVSGKYYIIDPKYKDKSLKFNPLNIEDSASNRNMLVELIRRMVADTSLVDVEEKIRKI
EEECCCEEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VDSIFAIPKESRSISQISEVLLLLGGKISRWCDDGDFAYLFQDGDESDIDWETKIISLNT
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEC
ANLTKQKECMSVILYYFLYSFEAKCDGSPAILVLDEAWEISNIFPTEKEFDNWMQRMTEL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
NVVVILSTENLNLAFASKFTQYLDKHVDTRILMPNVNANRLYMKAFSLSKEELNIILQTP
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEHHCCCCCCEEEEECC
TQEGLFLIKQYKGLVTLNLDLKDMQEIHVLSANKETIKYMYEAIKEKGEEVNEWLPVFYE
CCCCCEEEEECCCEEEEEECHHHHHHHHEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
KCKA
HHCC
>Mature Secondary Structure
MQLKVPTVNSKLKKKNNLALLESIDLDFVPYACHYDEETILTKQGELLKIIKLEDYSSVD
CEEECCCCCHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCEEEEEEECCCCCCC
NYSDLRTEIRKSISKNINSLYFTVWIHTVRKRNKLSLKWNKTEDFSDNLHSTWFNKLTDS
CHHHHHHHHHHHHHCCHHHEEEEEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
KLQYINELYIVILFSDFGKHTNNSFFFNRIKNKHKLSLQESHQELQKITDVIQNDLGPFG
HHHHHHHEEEEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AKKLSLRFSEGKIYSEMIEFLHYIVTLTHKDYPIHERDLSQYIRNFKIAFGFNTFQTIFE
CCEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHEEEEECCHHHHHHHH
NQQKFGSIFSIKEYREISLGNIDRCLQLESEFIITEIMIFTSNNKAIKEFKKQINMLQIS
CHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHCCEEEEC
EDNTLLKSSGIEEIIELEKTSSIDFCQQKIIFTIFADDRNKLAESISDLSSIMSLVGFMM
CCCCCHHHCCHHHHHHHHCCCCHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FRTDLHMESHFWAQLPGNFAFITQPKNILTKYACSFAMLHDFTSGTLKGRRWKEAVMIFF
HHHHCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEE
SKKGNPYFFNFHGKRNNGHTTILGAPNSGRTSLINFLLSESRKFNPRIVILDNTGKSIIF
ECCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEE
TKAVSGKYYIIDPKYKDKSLKFNPLNIEDSASNRNMLVELIRRMVADTSLVDVEEKIRKI
EEECCCEEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VDSIFAIPKESRSISQISEVLLLLGGKISRWCDDGDFAYLFQDGDESDIDWETKIISLNT
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEC
ANLTKQKECMSVILYYFLYSFEAKCDGSPAILVLDEAWEISNIFPTEKEFDNWMQRMTEL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
NVVVILSTENLNLAFASKFTQYLDKHVDTRILMPNVNANRLYMKAFSLSKEELNIILQTP
CEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEHHCCCCCCEEEEECC
TQEGLFLIKQYKGLVTLNLDLKDMQEIHVLSANKETIKYMYEAIKEKGEEVNEWLPVFYE
CCCCCEEEEECCCEEEEEECHHHHHHHHEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
KCKA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]