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The map label for this gene is comEC [H]

Identifier: 225630830

GI number: 225630830

Start: 1223768

End: 1225921

Strand: Direct

Name: comEC [H]

Synonym: WRi_011360

Alternate gene names: 225630830

Gene position: 1223768-1225921 (Clockwise)

Preceding gene: 225630829

Following gene: 225630831

Centisome position: 84.64

GC content: 34.22

Gene sequence:

>2154_bases
ATGCAAATATCGACACACTTATTAACTTTTCTTGACATAATAGTTGGTTATATACGTAACAATCTGCGCAATGAACAATA
TAACTTGATACTGTGGTTTCCTGTTTTTCAGTGTGTAGGAATTCTAACCTATTTTTCCCTAAGTTTTGAACCAAGCTGCA
TATCTATTTTTCTTTTGCTTTTACCCACACTAATTTTGATTGCAATATTGTATAAAAGGTACGCGATATTGTGTATCGCT
TTAATTGCAGTGCTCATAGGATTTACAGCTAGTAAATTAAGAACAGCTTTAGTTGATACTCAAATTCTTGATAAAGAGAG
ATATGTAAAAGATATCGTTGCCACAGTGAAGGATATTAACGACAGGGGCTCATATAAACAATTTCTGCTTTCTGTTGAAA
AATCCCCTACTATCTCAAAATCCTCTCCTGTCATCCCAGCGCCCCCTTTTGTCATCCCAGTACTTGATACTGGGATCCAG
AAAAAAAAGATATGGATTCCAGCGTCACGCGCTGGAATGACACCGGATAGAGCTCTAGATAACATCAGAATATCAGTTAG
AACCAAAGTGGAAAAAGGCATTAAAATAGGCGATCAAGTAAAATTATCAGCAAAACTCTTCCCTCTAAAGATTGCGCCCT
CGGAGTATGCATACGATTTTGCAAGAATAGCATATTACCAGAAAATAAGTGCAACAGGCTTTGCAACAAGCAAAATAGCC
CTGCACAAAAAGGCTGAAGCAAGGAAATTTCAAGAATATATAGAATCTTTCCGTCAATACATTTATGAAAACCTGCAGCA
AAATATCAAGAAACCACATGCGGATATAATCTCTGCATTACTGATCGGTAAAAAAGATGGCATAGATCAAAAAACTATGG
ACGCGATACGAGATTCAGGTATAGCACATTTGTTTGCCATATCTGGTTTGCATTTATCATTCGTTGCTGGTCTGTTTTTT
GTAGTGTTTCGTAATTTATTCGCAATATCTGAAACTTTGACTCTTAAGTATAACACCAAGAAAATATCTGCATTCCTTAC
TATCTTGCCAACCACGTTTTATTTGTTGATTACCGGTATGCAAATTTCTGCTCAGCGTGCCTACATCATGGTGATTTTAG
TACTCGTTGCAATAATGATAGAGAGAAAATATCGAGGGTTAATAGCAATTGCGTTTGCTGCTGCAGTGATACTTATCATA
GAACCTGAAGCAATTTTAAAGCCTGGCTTTCAGATGTCATTTTCTGCTGTTTTGGCACTGGTTGCCAGTTATCAAATTAA
TGCTAATAAATTATTCAAAATAAAAATAATGAAATATTTTGTGTCGATAATGATCAGCTCAGTAATAGCAAGTTTAGCAA
CTGTTCCGTATACGATATATAATTTTAATTATTTTTCAATTAGTGGCATTATCACAAATTTAGTTGCTATACCAATAGTT
ACATTAATTATTATTCCACTTGGAATAATTTATGTCTTATTGATTCCTTTAGGTATTGAATGGATTATAGCGCCATTTAT
AGAGCGCCCAATTAACAGCATTTTGTATATAACAAACGCTATCGCTAGTCTTCAGTATTTGGTTATTCCCATTCGTACTT
TTCCTGCCTCATCAATTATTATAATAACATTTGGGTTATTGTGGCTGTGCTTGTGGGAAAGAAATTGGCGTTTTTTGGGA
TTTTTTTTTATTGTACTAGGTATTTGCTTTAGCGCTGCATATAAAACCCCCGACATCCTAATAAATGCTGATAATGTTGC
TGCAAAGGAGAGTGATAACTTACTATATTCTCTCACTAGAAAAAGCAGAAATTTTGTTATCAAAACGTGGGCAAAGCAAA
ACGGACAGAACCAAATTTTGAATCATACAAAATATAGCAACCCAGATAAAAGACTAAAATATGGCTGTATATACAATAAA
GGAAATAATAAGTCAGTGTTGCTAGCTTATAAAAAAGAAGATATTTCAGAAAATTGTGATAAAGTTGATTTAACAATTCA
ATTAAGTGAGTTTGACTATTCAGCTTGCAACACTAAAATTATTAAACATGCTGATCTAGAAACGTATGGCACACATTCTG
TTTGGTTAACAAATCGTTACGTAAAGATTAATACAGTGCGCTCCAATAGGCCTTGGCACATGTTGAAAAATTAA

Upstream 100 bases:

>100_bases
TATGAGCCTGACGAGCTACCGAGCTGCTCCATCCCGCGTCGTTGCTTATTTAGTTATTATATATACAAATAATGAAAAAA
GAAAAATAAAATTTCACCAC

Downstream 100 bases:

>100_bases
GAAACTTGATTTTAAGGTTTGGTTAATGTATACCTAAATAAGTTTAAACTAGAAAAATGTTATGGTTACAGACACTCAAG
ATCCTTTCAAAGACTATTTT

Product: ComEC/Rec2 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MQISTHLLTFLDIIVGYIRNNLRNEQYNLILWFPVFQCVGILTYFSLSFEPSCISIFLLLLPTLILIAILYKRYAILCIA
LIAVLIGFTASKLRTALVDTQILDKERYVKDIVATVKDINDRGSYKQFLLSVEKSPTISKSSPVIPAPPFVIPVLDTGIQ
KKKIWIPASRAGMTPDRALDNIRISVRTKVEKGIKIGDQVKLSAKLFPLKIAPSEYAYDFARIAYYQKISATGFATSKIA
LHKKAEARKFQEYIESFRQYIYENLQQNIKKPHADIISALLIGKKDGIDQKTMDAIRDSGIAHLFAISGLHLSFVAGLFF
VVFRNLFAISETLTLKYNTKKISAFLTILPTTFYLLITGMQISAQRAYIMVILVLVAIMIERKYRGLIAIAFAAAVILII
EPEAILKPGFQMSFSAVLALVASYQINANKLFKIKIMKYFVSIMISSVIASLATVPYTIYNFNYFSISGIITNLVAIPIV
TLIIIPLGIIYVLLIPLGIEWIIAPFIERPINSILYITNAIASLQYLVIPIRTFPASSIIIITFGLLWLCLWERNWRFLG
FFFIVLGICFSAAYKTPDILINADNVAAKESDNLLYSLTRKSRNFVIKTWAKQNGQNQILNHTKYSNPDKRLKYGCIYNK
GNNKSVLLAYKKEDISENCDKVDLTIQLSEFDYSACNTKIIKHADLETYGTHSVWLTNRYVKINTVRSNRPWHMLKN

Sequences:

>Translated_717_residues
MQISTHLLTFLDIIVGYIRNNLRNEQYNLILWFPVFQCVGILTYFSLSFEPSCISIFLLLLPTLILIAILYKRYAILCIA
LIAVLIGFTASKLRTALVDTQILDKERYVKDIVATVKDINDRGSYKQFLLSVEKSPTISKSSPVIPAPPFVIPVLDTGIQ
KKKIWIPASRAGMTPDRALDNIRISVRTKVEKGIKIGDQVKLSAKLFPLKIAPSEYAYDFARIAYYQKISATGFATSKIA
LHKKAEARKFQEYIESFRQYIYENLQQNIKKPHADIISALLIGKKDGIDQKTMDAIRDSGIAHLFAISGLHLSFVAGLFF
VVFRNLFAISETLTLKYNTKKISAFLTILPTTFYLLITGMQISAQRAYIMVILVLVAIMIERKYRGLIAIAFAAAVILII
EPEAILKPGFQMSFSAVLALVASYQINANKLFKIKIMKYFVSIMISSVIASLATVPYTIYNFNYFSISGIITNLVAIPIV
TLIIIPLGIIYVLLIPLGIEWIIAPFIERPINSILYITNAIASLQYLVIPIRTFPASSIIIITFGLLWLCLWERNWRFLG
FFFIVLGICFSAAYKTPDILINADNVAAKESDNLLYSLTRKSRNFVIKTWAKQNGQNQILNHTKYSNPDKRLKYGCIYNK
GNNKSVLLAYKKEDISENCDKVDLTIQLSEFDYSACNTKIIKHADLETYGTHSVWLTNRYVKINTVRSNRPWHMLKN
>Mature_717_residues
MQISTHLLTFLDIIVGYIRNNLRNEQYNLILWFPVFQCVGILTYFSLSFEPSCISIFLLLLPTLILIAILYKRYAILCIA
LIAVLIGFTASKLRTALVDTQILDKERYVKDIVATVKDINDRGSYKQFLLSVEKSPTISKSSPVIPAPPFVIPVLDTGIQ
KKKIWIPASRAGMTPDRALDNIRISVRTKVEKGIKIGDQVKLSAKLFPLKIAPSEYAYDFARIAYYQKISATGFATSKIA
LHKKAEARKFQEYIESFRQYIYENLQQNIKKPHADIISALLIGKKDGIDQKTMDAIRDSGIAHLFAISGLHLSFVAGLFF
VVFRNLFAISETLTLKYNTKKISAFLTILPTTFYLLITGMQISAQRAYIMVILVLVAIMIERKYRGLIAIAFAAAVILII
EPEAILKPGFQMSFSAVLALVASYQINANKLFKIKIMKYFVSIMISSVIASLATVPYTIYNFNYFSISGIITNLVAIPIV
TLIIIPLGIIYVLLIPLGIEWIIAPFIERPINSILYITNAIASLQYLVIPIRTFPASSIIIITFGLLWLCLWERNWRFLG
FFFIVLGICFSAAYKTPDILINADNVAAKESDNLLYSLTRKSRNFVIKTWAKQNGQNQILNHTKYSNPDKRLKYGCIYNK
GNNKSVLLAYKKEDISENCDKVDLTIQLSEFDYSACNTKIIKHADLETYGTHSVWLTNRYVKINTVRSNRPWHMLKN

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=286, Percent_Identity=28.3216783216783, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 81241; Mature: 81241

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISTHLLTFLDIIVGYIRNNLRNEQYNLILWFPVFQCVGILTYFSLSFEPSCISIFLLL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LPTLILIAILYKRYAILCIALIAVLIGFTASKLRTALVDTQILDKERYVKDIVATVKDIN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRGSYKQFLLSVEKSPTISKSSPVIPAPPFVIPVLDTGIQKKKIWIPASRAGMTPDRALD
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHC
NIRISVRTKVEKGIKIGDQVKLSAKLFPLKIAPSEYAYDFARIAYYQKISATGFATSKIA
CEEEEEEEEHHCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LHKKAEARKFQEYIESFRQYIYENLQQNIKKPHADIISALLIGKKDGIDQKTMDAIRDSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
IAHLFAISGLHLSFVAGLFFVVFRNLFAISETLTLKYNTKKISAFLTILPTTFYLLITGM
CEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHCCC
QISAQRAYIMVILVLVAIMIERKYRGLIAIAFAAAVILIIEPEAILKPGFQMSFSAVLAL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCHHHHCCCCCHHHHHHHHH
VASYQINANKLFKIKIMKYFVSIMISSVIASLATVPYTIYNFNYFSISGIITNLVAIPIV
HHHCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEHHHHHHHHHHHHHH
TLIIIPLGIIYVLLIPLGIEWIIAPFIERPINSILYITNAIASLQYLVIPIRTFPASSII
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHH
IITFGLLWLCLWERNWRFLGFFFIVLGICFSAAYKTPDILINADNVAAKESDNLLYSLTR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEC
KSRNFVIKTWAKQNGQNQILNHTKYSNPDKRLKYGCIYNKGNNKSVLLAYKKEDISENCD
CCCCEEEEEECCCCCCHHHHCCCCCCCCCHHEEEEEEEECCCCCEEEEEEECCCCCCCCC
KVDLTIQLSEFDYSACNTKIIKHADLETYGTHSVWLTNRYVKINTVRSNRPWHMLKN
EEEEEEEEECCCCCCCCCHHEEECCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MQISTHLLTFLDIIVGYIRNNLRNEQYNLILWFPVFQCVGILTYFSLSFEPSCISIFLLL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LPTLILIAILYKRYAILCIALIAVLIGFTASKLRTALVDTQILDKERYVKDIVATVKDIN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRGSYKQFLLSVEKSPTISKSSPVIPAPPFVIPVLDTGIQKKKIWIPASRAGMTPDRALD
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHC
NIRISVRTKVEKGIKIGDQVKLSAKLFPLKIAPSEYAYDFARIAYYQKISATGFATSKIA
CEEEEEEEEHHCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LHKKAEARKFQEYIESFRQYIYENLQQNIKKPHADIISALLIGKKDGIDQKTMDAIRDSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
IAHLFAISGLHLSFVAGLFFVVFRNLFAISETLTLKYNTKKISAFLTILPTTFYLLITGM
CEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHCCC
QISAQRAYIMVILVLVAIMIERKYRGLIAIAFAAAVILIIEPEAILKPGFQMSFSAVLAL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCHHHHCCCCCHHHHHHHHH
VASYQINANKLFKIKIMKYFVSIMISSVIASLATVPYTIYNFNYFSISGIITNLVAIPIV
HHHCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEHHHHHHHHHHHHHH
TLIIIPLGIIYVLLIPLGIEWIIAPFIERPINSILYITNAIASLQYLVIPIRTFPASSII
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHH
IITFGLLWLCLWERNWRFLGFFFIVLGICFSAAYKTPDILINADNVAAKESDNLLYSLTR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEC
KSRNFVIKTWAKQNGQNQILNHTKYSNPDKRLKYGCIYNKGNNKSVLLAYKKEDISENCD
CCCCEEEEEECCCCCCHHHHCCCCCCCCCHHEEEEEEEECCCCCEEEEEEECCCCCCCCC
KVDLTIQLSEFDYSACNTKIIKHADLETYGTHSVWLTNRYVKINTVRSNRPWHMLKN
EEEEEEEEECCCCCCCCCHHEEECCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]