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The map label for this gene is pccB [H]

Identifier: 225630826

GI number: 225630826

Start: 1220419

End: 1221945

Strand: Direct

Name: pccB [H]

Synonym: WRi_011300

Alternate gene names: 225630826

Gene position: 1220419-1221945 (Clockwise)

Preceding gene: 225630825

Following gene: 225630827

Centisome position: 84.41

GC content: 36.21

Gene sequence:

>1527_bases
ATGCAAGACTTTAAAATACTAGAAAATCTAAAAACCAAAGCACTAGAAGCTGAAAAAGGAGGCGGTTCTGACAGGATTAC
CAAACAACATGAAAAAGGGAAATTAACTGCTAGAGAAAGGCTCAGTATATTGCTCGATGAAAGTTCTTTTGCAGAATACG
ATAAATTCGTAAAACATCATGCAAATGATTTTGGCATGCAAAATGCTAATTTTTTAGGTGATGGGGTAGTGATTGGTCAT
GGTACTATTTATGGCAGAAAAGTTTTTGTTTATTCTCAGGATTTTACTGTCTTTGGTGGTTCACTTGGTGCGTCACATGC
AAAAAAAATATGCAAAATTATGGATATGGCAATTAATGCCAGAGTTCCAATTATCGGACTAAATGATTCTGGTGGAGCCA
GAATTCAGGAAGGAGTAAATTCTCTTGCTGGTTATGGAGAAATTTTTCAAAGAAACGTAAATGCATCGGGTGTTATACCA
CAAATTTCTTTAATTATGGGCCCATGTGCAGGAGGTGCAGTTTATTCCCCAGCATTAACTGACTTTACTTTCATGGTGAA
AAACAGTTCATATATGTTTATAACCGGGCCAGATGTAGTAAAAAAGGTTACCTACGAAGATGTAAGTTTCGAAGACCTCG
GTGGAGCAAAAATTCATACAAGCAAAACAGGAGTAGCGGATTTTGCATTCAATAATGATGTTGAAATGCTGCTAAAAATG
CGTGAATTCTTTACTTTCTTACCAGCAAATAATCAAGAATCACCAAAATCTGCACCAATCTGTAATGATGTTGACGACAT
TGATGAATCCTTAAACACTCTAATTCCTACCAATCCTAATACTCCTTATGATATGTATGAACTCATTGAAAAGGTGTGCG
ATGAGAGGAATTTCTTTGAACTGAAGCCTGATTTTGCTCGTAATATCATAACTGGTTTTGGTAGAATTGGAGGAAATACT
GTCGGTATTGTTGCAAATCAACCTATGCACCTTGCAGGATGTTTGGATATTGATTCTTCAAGAAAAGCTGCAAGGTTTGT
AAGGTTTTGTGACGCATTTAACATTCCCATCATCACACTTATTGATGTTCCAGGATTTCTGCCAGGTACAAATCAAGAAT
ACAATAATATAATACAACACGGAGCGAAACTGCTTTACGCTTACGCTGAAGCGACCGTGCCAAAAATTAGCCTTATCACT
AGAAAAGCGTATGGTGGTGCATACATTGTTATGAATTCGAAACATCTAAAAGGTGATATAAATTATGCCTGGCCAACTGC
TGAAATAGCTGTGATGGGCCCTGAAAGTGCAGTTGAAATTATATTTAGGCATGAAAAAGACCAGCAAACGTTAATCAAAG
AATATAAAGAGAAATTTGCTAATCCATTTTTTGCTGCATCACATGGATACATTGATGACATTATAGTACCAAGTAAAACA
AGGTATCACTTTTGTAAAGCATTAGAGCTACTCAAAAACAAGAAGGTAGAAAGGATATGGAAAAAGCATGACAACTTACC
TCTGTAA

Upstream 100 bases:

>100_bases
TACACATACACTGTTGCATATAGCAATAGTGTATGTGTATAGTTAGTAGGGTTTGTAAACTTAATAAAGTCTACAACTAA
TTTGAAAACTACTTTATTGT

Downstream 100 bases:

>100_bases
CTTTCTTCTAAGCCTTGCTTACAAGTTATGCAATATTATAAGTGTAATCAGTGTGCCGTTACCAAGACTCGAACTTGGGA
CCTCGTCATTACCAATGACG

Product: propionyl-CoA carboxylase, beta subunit

Products: NA

Alternate protein names: PCCase; Propanoyl-CoA:carbon dioxide ligase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQDFKILENLKTKALEAEKGGGSDRITKQHEKGKLTARERLSILLDESSFAEYDKFVKHHANDFGMQNANFLGDGVVIGH
GTIYGRKVFVYSQDFTVFGGSLGASHAKKICKIMDMAINARVPIIGLNDSGGARIQEGVNSLAGYGEIFQRNVNASGVIP
QISLIMGPCAGGAVYSPALTDFTFMVKNSSYMFITGPDVVKKVTYEDVSFEDLGGAKIHTSKTGVADFAFNNDVEMLLKM
REFFTFLPANNQESPKSAPICNDVDDIDESLNTLIPTNPNTPYDMYELIEKVCDERNFFELKPDFARNIITGFGRIGGNT
VGIVANQPMHLAGCLDIDSSRKAARFVRFCDAFNIPIITLIDVPGFLPGTNQEYNNIIQHGAKLLYAYAEATVPKISLIT
RKAYGGAYIVMNSKHLKGDINYAWPTAEIAVMGPESAVEIIFRHEKDQQTLIKEYKEKFANPFFAASHGYIDDIIVPSKT
RYHFCKALELLKNKKVERIWKKHDNLPL

Sequences:

>Translated_508_residues
MQDFKILENLKTKALEAEKGGGSDRITKQHEKGKLTARERLSILLDESSFAEYDKFVKHHANDFGMQNANFLGDGVVIGH
GTIYGRKVFVYSQDFTVFGGSLGASHAKKICKIMDMAINARVPIIGLNDSGGARIQEGVNSLAGYGEIFQRNVNASGVIP
QISLIMGPCAGGAVYSPALTDFTFMVKNSSYMFITGPDVVKKVTYEDVSFEDLGGAKIHTSKTGVADFAFNNDVEMLLKM
REFFTFLPANNQESPKSAPICNDVDDIDESLNTLIPTNPNTPYDMYELIEKVCDERNFFELKPDFARNIITGFGRIGGNT
VGIVANQPMHLAGCLDIDSSRKAARFVRFCDAFNIPIITLIDVPGFLPGTNQEYNNIIQHGAKLLYAYAEATVPKISLIT
RKAYGGAYIVMNSKHLKGDINYAWPTAEIAVMGPESAVEIIFRHEKDQQTLIKEYKEKFANPFFAASHGYIDDIIVPSKT
RYHFCKALELLKNKKVERIWKKHDNLPL
>Mature_508_residues
MQDFKILENLKTKALEAEKGGGSDRITKQHEKGKLTARERLSILLDESSFAEYDKFVKHHANDFGMQNANFLGDGVVIGH
GTIYGRKVFVYSQDFTVFGGSLGASHAKKICKIMDMAINARVPIIGLNDSGGARIQEGVNSLAGYGEIFQRNVNASGVIP
QISLIMGPCAGGAVYSPALTDFTFMVKNSSYMFITGPDVVKKVTYEDVSFEDLGGAKIHTSKTGVADFAFNNDVEMLLKM
REFFTFLPANNQESPKSAPICNDVDDIDESLNTLIPTNPNTPYDMYELIEKVCDERNFFELKPDFARNIITGFGRIGGNT
VGIVANQPMHLAGCLDIDSSRKAARFVRFCDAFNIPIITLIDVPGFLPGTNQEYNNIIQHGAKLLYAYAEATVPKISLIT
RKAYGGAYIVMNSKHLKGDINYAWPTAEIAVMGPESAVEIIFRHEKDQQTLIKEYKEKFANPFFAASHGYIDDIIVPSKT
RYHFCKALELLKNKKVERIWKKHDNLPL

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI119943100, Length=510, Percent_Identity=63.1372549019608, Blast_Score=659, Evalue=0.0,
Organism=Homo sapiens, GI295821216, Length=530, Percent_Identity=60.7547169811321, Blast_Score=647, Evalue=0.0,
Organism=Homo sapiens, GI11545863, Length=520, Percent_Identity=31.3461538461538, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI134142062, Length=408, Percent_Identity=25.7352941176471, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI38679977, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38679967, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38679971, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38679960, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38679974, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788655, Length=255, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25147362, Length=506, Percent_Identity=61.4624505928854, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147359, Length=506, Percent_Identity=61.4624505928854, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17552936, Length=498, Percent_Identity=31.3253012048193, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71997163, Length=365, Percent_Identity=24.6575342465753, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323863, Length=357, Percent_Identity=24.9299719887955, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586065, Length=513, Percent_Identity=31.9688109161793, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000438
- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: =6.4.1.3 [H]

Molecular weight: Translated: 56420; Mature: 56420

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDFKILENLKTKALEAEKGGGSDRITKQHEKGKLTARERLSILLDESSFAEYDKFVKHH
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHEEEECCCHHHHHHHHHHHC
ANDFGMQNANFLGDGVVIGHGTIYGRKVFVYSQDFTVFGGSLGASHAKKICKIMDMAINA
CHHCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCC
RVPIIGLNDSGGARIQEGVNSLAGYGEIFQRNVNASGVIPQISLIMGPCAGGAVYSPALT
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHH
DFTFMVKNSSYMFITGPDVVKKVTYEDVSFEDLGGAKIHTSKTGVADFAFNNDVEMLLKM
CEEEEEECCCEEEEECHHHHHHHHHCCCCHHCCCCCEEECCCCCCEEEECCCCHHHHHHH
REFFTFLPANNQESPKSAPICNDVDDIDESLNTLIPTNPNTPYDMYELIEKVCDERNFFE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEE
LKPDFARNIITGFGRIGGNTVGIVANQPMHLAGCLDIDSSRKAARFVRFCDAFNIPIITL
ECHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
IDVPGFLPGTNQEYNNIIQHGAKLLYAYAEATVPKISLITRKAYGGAYIVMNSKHLKGDI
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCC
NYAWPTAEIAVMGPESAVEIIFRHEKDQQTLIKEYKEKFANPFFAASHGYIDDIIVPSKT
EEECCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCH
RYHFCKALELLKNKKVERIWKKHDNLPL
HHHHHHHHHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MQDFKILENLKTKALEAEKGGGSDRITKQHEKGKLTARERLSILLDESSFAEYDKFVKHH
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHEEEECCCHHHHHHHHHHHC
ANDFGMQNANFLGDGVVIGHGTIYGRKVFVYSQDFTVFGGSLGASHAKKICKIMDMAINA
CHHCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCC
RVPIIGLNDSGGARIQEGVNSLAGYGEIFQRNVNASGVIPQISLIMGPCAGGAVYSPALT
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHH
DFTFMVKNSSYMFITGPDVVKKVTYEDVSFEDLGGAKIHTSKTGVADFAFNNDVEMLLKM
CEEEEEECCCEEEEECHHHHHHHHHCCCCHHCCCCCEEECCCCCCEEEECCCCHHHHHHH
REFFTFLPANNQESPKSAPICNDVDDIDESLNTLIPTNPNTPYDMYELIEKVCDERNFFE
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEE
LKPDFARNIITGFGRIGGNTVGIVANQPMHLAGCLDIDSSRKAARFVRFCDAFNIPIITL
ECHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
IDVPGFLPGTNQEYNNIIQHGAKLLYAYAEATVPKISLITRKAYGGAYIVMNSKHLKGDI
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCC
NYAWPTAEIAVMGPESAVEIIFRHEKDQQTLIKEYKEKFANPFFAASHGYIDDIIVPSKT
EEECCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCH
RYHFCKALELLKNKKVERIWKKHDNLPL
HHHHHHHHHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830278 [H]