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The map label for this gene is trmB [H]

Identifier: 225630821

GI number: 225630821

Start: 1217217

End: 1217882

Strand: Direct

Name: trmB [H]

Synonym: WRi_011250

Alternate gene names: 225630821

Gene position: 1217217-1217882 (Clockwise)

Preceding gene: 225630817

Following gene: 225630824

Centisome position: 84.19

GC content: 33.18

Gene sequence:

>666_bases
ATGCTATTTAAGAATAGCAAGTGGATTAGATCGTTCTCCAGAAGGTCCAGGCTAAAACCTGATGTTGATGAAATATTGGA
AAAATATTCTATTCAAAACAGCAAGGAATCTATAGAGAAAATTGTAAATTCACAAAAAAGAATATGGGTAGAAATAGGCT
TTGGCAACGGTGAAAATATGCTTTACCAGGTGCTCAATGAACCTGATCTATTATTTATAGGATGTGAGCCCTACTTAAAG
GGGGTTTCTCGCTTGCTGACAAACATAGAAATACAGAACATAAAAAATATTTTAATGTGGACAGAAGATGCAAGAGAATT
GATTGCAAATTTTCCTGATAACAGTGTTGAAAGATTCTTTATTCTCTTTCCAGATCCATGGCCAAAAAGAAGTCACAATA
AAAGACGATTAATTAATACGGAATTTTTAAATTTATTAGCAAAAAAAATACTTATAACAGGGGAAATATTCATTGCAACC
GATCATCAGGACTATGCAGAGTGGATAGCATCACATATAAAGCAGTGTAACTCTTTAATCTATAGGGAAGACGATTTCAC
AAGTTATACTCTTACAAAATACCACAGAAGAGCGCTCAAAGATCAGCGTAAAGTGAGGTTCTTTAAAGTAAGTGTTATTA
ATAATTTGCAGACTATGGATCAATAG

Upstream 100 bases:

>100_bases
AATATTAACAATTATTGACAAGTAATTTTTCATTAACTCATTTCGAGAATTGTTTAAGCTATATAAAATATAGTGAGAAA
ATTATATATGTATCAAGAAT

Downstream 100 bases:

>100_bases
AGTTTTTGCATATCTCTTTTACGTTAACTATAGATTAAAAATAATAAAAAAAGAGGGAGAAATAGAGTAAATTCCTTTAA
CTACTTCTCCTTATGAAGGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLFKNSKWIRSFSRRSRLKPDVDEILEKYSIQNSKESIEKIVNSQKRIWVEIGFGNGENMLYQVLNEPDLLFIGCEPYLK
GVSRLLTNIEIQNIKNILMWTEDARELIANFPDNSVERFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILITGEIFIAT
DHQDYAEWIASHIKQCNSLIYREDDFTSYTLTKYHRRALKDQRKVRFFKVSVINNLQTMDQ

Sequences:

>Translated_221_residues
MLFKNSKWIRSFSRRSRLKPDVDEILEKYSIQNSKESIEKIVNSQKRIWVEIGFGNGENMLYQVLNEPDLLFIGCEPYLK
GVSRLLTNIEIQNIKNILMWTEDARELIANFPDNSVERFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILITGEIFIAT
DHQDYAEWIASHIKQCNSLIYREDDFTSYTLTKYHRRALKDQRKVRFFKVSVINNLQTMDQ
>Mature_221_residues
MLFKNSKWIRSFSRRSRLKPDVDEILEKYSIQNSKESIEKIVNSQKRIWVEIGFGNGENMLYQVLNEPDLLFIGCEPYLK
GVSRLLTNIEIQNIKNILMWTEDARELIANFPDNSVERFFILFPDPWPKRSHNKRRLINTEFLNLLAKKILITGEIFIAT
DHQDYAEWIASHIKQCNSLIYREDDFTSYTLTKYHRRALKDQRKVRFFKVSVINNLQTMDQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=167, Percent_Identity=32.3353293413174, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26351; Mature: 26351

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFKNSKWIRSFSRRSRLKPDVDEILEKYSIQNSKESIEKIVNSQKRIWVEIGFGNGENM
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHH
LYQVLNEPDLLFIGCEPYLKGVSRLLTNIEIQNIKNILMWTEDARELIANFPDNSVERFF
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEE
ILFPDPWPKRSHNKRRLINTEFLNLLAKKILITGEIFIATDHQDYAEWIASHIKQCNSLI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHE
YREDDFTSYTLTKYHRRALKDQRKVRFFKVSVINNLQTMDQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCC
>Mature Secondary Structure
MLFKNSKWIRSFSRRSRLKPDVDEILEKYSIQNSKESIEKIVNSQKRIWVEIGFGNGENM
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHH
LYQVLNEPDLLFIGCEPYLKGVSRLLTNIEIQNIKNILMWTEDARELIANFPDNSVERFF
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEE
ILFPDPWPKRSHNKRRLINTEFLNLLAKKILITGEIFIATDHQDYAEWIASHIKQCNSLI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHE
YREDDFTSYTLTKYHRRALKDQRKVRFFKVSVINNLQTMDQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA