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The map label for this gene is rpoH [H]

Identifier: 225630797

GI number: 225630797

Start: 1190187

End: 1190999

Strand: Direct

Name: rpoH [H]

Synonym: WRi_011000

Alternate gene names: 225630797

Gene position: 1190187-1190999 (Clockwise)

Preceding gene: 225630796

Following gene: 225630798

Centisome position: 82.32

GC content: 34.07

Gene sequence:

>813_bases
ATGCTGTCTCAAGAGGAAGAGGTACGATTAGCAAAAAATTGGAACCAATATAAGGATGTTTCTTCTGCTCATAAATTGAT
TACCAGCCATTTGAATTTGGTAGTGAAAATTGCAATGAAATTCAAAAATTATGGACTATTGTTGATGGACCTGGTCATGG
AAGGAAATATGGGTTTAATGCATGCAGTGAAAAAGTTTAATCCTGATTTAGGGTTTCGTTTTTCAACTTATGCGGTGTGG
TGGATTGAAGCTTCAATAAAAGACTTTATATTGAAATCTTGGTCGTTTGTGAAAATAGGCACAACGCAAGCACAAAGAAG
ATTGTTTTTTAGTTTACGTAAAATAAAAAAAAGAATTTTACAGTATACAAACAGGGAAACCTTAAATAATGAAGAAATAA
AAGCAATATCTAATGAGCTTTCTGTATCCGAAGGAGACGTTCTGCAGATGAATTACCGTATGGCTTGTAAGGATCAGTCG
CTAAACGATTGCATAAAAATAGGTGATGACTCTAAAAGGGAGCTACAAGATATGATCCCATGTAACTTGGTTAGTCAAGA
AGTAACCTATCAGAATCATGAAGAAAAAGAATTAAAAGAAGCAATTTTAAACAATGCACTAGCGAGCCTCAACGAAAGGG
CAAGAGACATTTTTATCAGTAGATATTTGTCTGAAAACATTCAAACTCTGGATGAACTTAGTAAAAAATATTGTGTTTCA
AGAGAAAGAATAAGGCAGTTGGCTGAGCAGGCGATGGCTAAGGTAAAACAATATATACAATCTGAAAGTTTGAAATTTTG
TTTGCATGCGTAG

Upstream 100 bases:

>100_bases
AGTTTTAATTTAAGTTGTTATGTTGACCTCAATGGCAAACTTATGTGTCGATAGCAGAACCAATCTCATGCAATATATTG
CTAGGGTGCAAAAATTTCCT

Downstream 100 bases:

>100_bases
TTTTTTTATATTTCTAATATTTTTTTCGATAGGCGCTTTTGCATCGGTTAGTGATGTACAATTGAAGGAAAAATATAAAG
ATTGGCTCGTGTATACTGCA

Product: heat shock sigma factor RpoH

Products: diphosphate; RNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLSQEEEVRLAKNWNQYKDVSSAHKLITSHLNLVVKIAMKFKNYGLLLMDLVMEGNMGLMHAVKKFNPDLGFRFSTYAVW
WIEASIKDFILKSWSFVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLNNEEIKAISNELSVSEGDVLQMNYRMACKDQS
LNDCIKIGDDSKRELQDMIPCNLVSQEVTYQNHEEKELKEAILNNALASLNERARDIFISRYLSENIQTLDELSKKYCVS
RERIRQLAEQAMAKVKQYIQSESLKFCLHA

Sequences:

>Translated_270_residues
MLSQEEEVRLAKNWNQYKDVSSAHKLITSHLNLVVKIAMKFKNYGLLLMDLVMEGNMGLMHAVKKFNPDLGFRFSTYAVW
WIEASIKDFILKSWSFVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLNNEEIKAISNELSVSEGDVLQMNYRMACKDQS
LNDCIKIGDDSKRELQDMIPCNLVSQEVTYQNHEEKELKEAILNNALASLNERARDIFISRYLSENIQTLDELSKKYCVS
RERIRQLAEQAMAKVKQYIQSESLKFCLHA
>Mature_270_residues
MLSQEEEVRLAKNWNQYKDVSSAHKLITSHLNLVVKIAMKFKNYGLLLMDLVMEGNMGLMHAVKKFNPDLGFRFSTYAVW
WIEASIKDFILKSWSFVKIGTTQAQRRLFFSLRKIKKRILQYTNRETLNNEEIKAISNELSVSEGDVLQMNYRMACKDQS
LNDCIKIGDDSKRELQDMIPCNLVSQEVTYQNHEEKELKEAILNNALASLNERARDIFISRYLSENIQTLDELSKKYCVS
RERIRQLAEQAMAKVKQYIQSESLKFCLHA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=269, Percent_Identity=39.0334572490706, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=31.2267657992565, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789448, Length=246, Percent_Identity=31.3008130081301, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 31534; Mature: 31534

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQEEEVRLAKNWNQYKDVSSAHKLITSHLNLVVKIAMKFKNYGLLLMDLVMEGNMGLM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
HAVKKFNPDLGFRFSTYAVWWIEASIKDFILKSWSFVKIGTTQAQRRLFFSLRKIKKRIL
HHHHHCCCCCCCEEEHEEEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
QYTNRETLNNEEIKAISNELSVSEGDVLQMNYRMACKDQSLNDCIKIGDDSKRELQDMIP
HHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCHHHHHHCCCCCHHHHHHCCC
CNLVSQEVTYQNHEEKELKEAILNNALASLNERARDIFISRYLSENIQTLDELSKKYCVS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERIRQLAEQAMAKVKQYIQSESLKFCLHA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MLSQEEEVRLAKNWNQYKDVSSAHKLITSHLNLVVKIAMKFKNYGLLLMDLVMEGNMGLM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
HAVKKFNPDLGFRFSTYAVWWIEASIKDFILKSWSFVKIGTTQAQRRLFFSLRKIKKRIL
HHHHHCCCCCCCEEEHEEEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
QYTNRETLNNEEIKAISNELSVSEGDVLQMNYRMACKDQSLNDCIKIGDDSKRELQDMIP
HHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCHHHHHHCCCCCHHHHHHCCC
CNLVSQEVTYQNHEEKELKEAILNNALASLNERARDIFISRYLSENIQTLDELSKKYCVS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERIRQLAEQAMAKVKQYIQSESLKFCLHA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]