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The map label for this gene is dagA [H]

Identifier: 225630780

GI number: 225630780

Start: 1173231

End: 1174574

Strand: Reverse

Name: dagA [H]

Synonym: WRi_010800

Alternate gene names: 225630780

Gene position: 1174574-1173231 (Counterclockwise)

Preceding gene: 225630781

Following gene: 225630779

Centisome position: 81.24

GC content: 34.3

Gene sequence:

>1344_bases
ATGATTGTAAAAGTAACTGATCTATTAAACTCTCTTCTATATATAAAGATATTCAACATTCCATTTATAATTATTTGGGT
AATTGCAACTGGAATCTTTTGTACTGCCCAGTTCAAATTCATTAACTTTAGGTTACTTAAATATGGAATACAGACACTAT
TCAATCTAAAGTGTAATAACAGCAACAATGGCATAATCACTCATATTCAAGCGTTTGCAACAGTAATTTCAGGAACAGTT
GGTCTTGGAACAATATCAGGAGTTGCAATAGCTATCACAATAGGGGGCCCAAGTGCAGTTTTTTGGATGGTAATTACCGG
AATACTTGGTATGTCGATAAAGTTTGCCGAGGTGGTGCTTGCATTCACTTATCGCTCTGAAAATACAACTGGTGGTGCTT
TTTATTACATGGAATACGGGCTTGCAAAGATTGGATTTGCAAAAACTGGTAGATTTCTTGCCTTCACTTATGCGATTATG
CTACTTATTGCAATGATTTTGGGCGGTATACCATTTCAAGCAAATCAAATAGCAGCACTATCAAATAACCTCCTAGAATA
CAACGCATCCATTATTATATCCCTGCTTGTCTTTATTGTAATATTGGGAGGAATAAAGCGCATTGCTTTCGTTTCAACGA
GCTTAGCACCAATTATGATAGTGCTATATGTGGGTATGTGTATATATTTAATTTATGTAAACAGAAGTAATTTGCTGAAT
GCTTTATCTATAATATTTCAAGACATTTTCAATAAATCTGCTATAGGAGGCGGAGTATTGAGCGGATTAATAGCCGGAGT
GAGAAGATCAGTGTTTGCTAACGAAGCAGGTACCGGAACAGCAGCTATAGCACATTCAGCTGTAAAAGAAGAAGATCCAA
TTAAAGTTGGGTGCGTTGCAATGATTGCACCGCTTATAGACACAATATTAATCTCATTTTTGACTGGTATTGTGATAATT
ATCACTGGTATGCACAGTACTGATAACGTGGGTGATATTACACTAATTAGTTCGGTATTTTCAACAGCTTTGCCCTTGTT
TAGCAAGTTAGTTTTTCCGCTAATGATGTTTTCCTTTGCATTTTCCACAATAATAGCTTATTGTTACTACTGTGAAGTTG
CTTTGCTGTACTTATTTGGTAATAAAAAAATACTGATACTGTTCCAAATTCTTATAGTGGTTTCAGTGTATATCAGTTGT
ATGTCAAAGAATATAGAATTTATATCTTATCTAGGTGACAGTTTGTTTATGTGCCTTATGATTCCAAATGCTGCTGCAAT
ATATCTACTAAGAAGGGAAGTTTTGAATACAATAGATTCTTATTACAATTCTAAAAGGTATTAA

Upstream 100 bases:

>100_bases
TTTTAGGTATAGTGTATACACTTACTATTCCAATAAGCATCGGGTTTTACATATATTTTACATATAAAACTCGTTAGCTA
TATCAAAAGTAGTGAGCAGC

Downstream 100 bases:

>100_bases
CATGATTTATAGATTGCTTTGTATGGTGTTATTTTGCCTATTGTTTAATGATGTGTACGCTGCTTCAGGTTTTTACGAAG
GTTATGATGCTGTGTTGAAT

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIVKVTDLLNSLLYIKIFNIPFIIIWVIATGIFCTAQFKFINFRLLKYGIQTLFNLKCNNSNNGIITHIQAFATVISGTV
GLGTISGVAIAITIGGPSAVFWMVITGILGMSIKFAEVVLAFTYRSENTTGGAFYYMEYGLAKIGFAKTGRFLAFTYAIM
LLIAMILGGIPFQANQIAALSNNLLEYNASIIISLLVFIVILGGIKRIAFVSTSLAPIMIVLYVGMCIYLIYVNRSNLLN
ALSIIFQDIFNKSAIGGGVLSGLIAGVRRSVFANEAGTGTAAIAHSAVKEEDPIKVGCVAMIAPLIDTILISFLTGIVII
ITGMHSTDNVGDITLISSVFSTALPLFSKLVFPLMMFSFAFSTIIAYCYYCEVALLYLFGNKKILILFQILIVVSVYISC
MSKNIEFISYLGDSLFMCLMIPNAAAIYLLRREVLNTIDSYYNSKRY

Sequences:

>Translated_447_residues
MIVKVTDLLNSLLYIKIFNIPFIIIWVIATGIFCTAQFKFINFRLLKYGIQTLFNLKCNNSNNGIITHIQAFATVISGTV
GLGTISGVAIAITIGGPSAVFWMVITGILGMSIKFAEVVLAFTYRSENTTGGAFYYMEYGLAKIGFAKTGRFLAFTYAIM
LLIAMILGGIPFQANQIAALSNNLLEYNASIIISLLVFIVILGGIKRIAFVSTSLAPIMIVLYVGMCIYLIYVNRSNLLN
ALSIIFQDIFNKSAIGGGVLSGLIAGVRRSVFANEAGTGTAAIAHSAVKEEDPIKVGCVAMIAPLIDTILISFLTGIVII
ITGMHSTDNVGDITLISSVFSTALPLFSKLVFPLMMFSFAFSTIIAYCYYCEVALLYLFGNKKILILFQILIVVSVYISC
MSKNIEFISYLGDSLFMCLMIPNAAAIYLLRREVLNTIDSYYNSKRY
>Mature_447_residues
MIVKVTDLLNSLLYIKIFNIPFIIIWVIATGIFCTAQFKFINFRLLKYGIQTLFNLKCNNSNNGIITHIQAFATVISGTV
GLGTISGVAIAITIGGPSAVFWMVITGILGMSIKFAEVVLAFTYRSENTTGGAFYYMEYGLAKIGFAKTGRFLAFTYAIM
LLIAMILGGIPFQANQIAALSNNLLEYNASIIISLLVFIVILGGIKRIAFVSTSLAPIMIVLYVGMCIYLIYVNRSNLLN
ALSIIFQDIFNKSAIGGGVLSGLIAGVRRSVFANEAGTGTAAIAHSAVKEEDPIKVGCVAMIAPLIDTILISFLTGIVII
ITGMHSTDNVGDITLISSVFSTALPLFSKLVFPLMMFSFAFSTIIAYCYYCEVALLYLFGNKKILILFQILIVVSVYISC
MSKNIEFISYLGDSLFMCLMIPNAAAIYLLRREVLNTIDSYYNSKRY

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=436, Percent_Identity=29.8165137614679, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48834; Mature: 48834

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKVTDLLNSLLYIKIFNIPFIIIWVIATGIFCTAQFKFINFRLLKYGIQTLFNLKCNN
CEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHEEEECC
SNNGIITHIQAFATVISGTVGLGTISGVAIAITIGGPSAVFWMVITGILGMSIKFAEVVL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHH
AFTYRSENTTGGAFYYMEYGLAKIGFAKTGRFLAFTYAIMLLIAMILGGIPFQANQIAAL
HHEECCCCCCCCEEEEEEHHHHHHCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SNNLLEYNASIIISLLVFIVILGGIKRIAFVSTSLAPIMIVLYVGMCIYLIYVNRSNLLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALSIIFQDIFNKSAIGGGVLSGLIAGVRRSVFANEAGTGTAAIAHSAVKEEDPIKVGCVA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
MIAPLIDTILISFLTGIVIIITGMHSTDNVGDITLISSVFSTALPLFSKLVFPLMMFSFA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTIIAYCYYCEVALLYLFGNKKILILFQILIVVSVYISCMSKNIEFISYLGDSLFMCLM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPNAAAIYLLRREVLNTIDSYYNSKRY
CCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVKVTDLLNSLLYIKIFNIPFIIIWVIATGIFCTAQFKFINFRLLKYGIQTLFNLKCNN
CEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHEEEECC
SNNGIITHIQAFATVISGTVGLGTISGVAIAITIGGPSAVFWMVITGILGMSIKFAEVVL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHH
AFTYRSENTTGGAFYYMEYGLAKIGFAKTGRFLAFTYAIMLLIAMILGGIPFQANQIAAL
HHEECCCCCCCCEEEEEEHHHHHHCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
SNNLLEYNASIIISLLVFIVILGGIKRIAFVSTSLAPIMIVLYVGMCIYLIYVNRSNLLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALSIIFQDIFNKSAIGGGVLSGLIAGVRRSVFANEAGTGTAAIAHSAVKEEDPIKVGCVA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
MIAPLIDTILISFLTGIVIIITGMHSTDNVGDITLISSVFSTALPLFSKLVFPLMMFSFA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTIIAYCYYCEVALLYLFGNKKILILFQILIVVSVYISCMSKNIEFISYLGDSLFMCLM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPNAAAIYLLRREVLNTIDSYYNSKRY
CCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]