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The map label for this gene is dagA [H]

Identifier: 225630779

GI number: 225630779

Start: 1171763

End: 1173229

Strand: Reverse

Name: dagA [H]

Synonym: WRi_010790

Alternate gene names: 225630779

Gene position: 1173229-1171763 (Counterclockwise)

Preceding gene: 225630780

Following gene: 225630778

Centisome position: 81.14

GC content: 36.26

Gene sequence:

>1467_bases
ATGATTTATAGATTGCTTTGTATGGTGTTATTTTGCCTATTGTTTAATGATGTGTACGCTGCTTCAGGTTTTTACGAAGG
TTATGATGCTGTGTTGAATGGAATAAATAACTTCATGAATGAAGTGCTGTTTTTCAAAATCTTTTATATACCATTCATAC
TTCTTCTACTATCCTTTGGTTATGTATTTTTAACCTTACGCTTTAAGTTTCTCAACATAAGAATGTTTAAGCATGCGTTT
GCTATTTTATGTGGAAAATATGACACGAATCACCATGATGGTCATATTACGCATTTTCAGGCATTTATGACTGCACTTTC
AAGCACAGTAGGTCTTGGAACTGTTGCTGGAGTCGCAATTGCAGTTTCAATGGGAGGGCCAGGAGCGGTGCCTTGGATGA
TGATTACAGGTTTTTTTGGTATGTCAGCAAAATTTGCTGAGGTAACGTTGGCGTTTAGACATAGAACAGAAGATCAGGAC
CAATTGTTTAGTGGTCCCTTTCAGTATATAAGGAATGGTCTGGAGGAATTTGGATTTAAAAAACTTGGTATTGTGCTGGC
TGCCATGTATGCAGTATTCTTTGTATTATCAGGTCTTGGTGGAAGTGTAGCGTTTCAAACCAATCAAATGGTTTCAATAT
TATCTGGCTATTCAAGTTGGGTAGATGGTCACTCTTGGTTTCTTTCTTCCATAATCTCTGCGCTGCTTGCTCTAGTGATT
ATTGGCGGAATTAAAAGGATAGCTAGAGTATCTTCTGCACTAGTGCCTATTATGTCACTTATATATATCTTTAGTTGTAT
TATTATTATTGCATTTAATATTCATAACCTTGGTTATACGTTAAAAATATTATTTTCCACTATGATAGATTTCAATGCTG
TTGGTGGAGGGATGGTAGGAGCATTTGTTGCTGGAATTCAAAGGGCAATCTTTGCCAGCGAAGCGGGTGTTGGTTCTGCT
TCAATTACTCATGCAACAACTAAAGATGAAGAACCAGTAAGAACTGGGCTTGTTGCTATGATAGAACCATGCTTTGACAC
AATGTTAATTTGCTGTTTAACAGGAATAACAATAGTAATAACAGGTGCATACCAGACTAGTGTTGGTGAAGGGATATTAA
TTACTCAAAAGGCATTTGAGACAGTTTCTCCATGGTTTCCTATACTTTTAACCATAGCTGCACCTTTGTTTGCATTTTCT
TCAGTAATTTCGTTTGTATATTGTTGTGAAATGGGGTGGCTATATTTATTCGGTGCAAAAAGTATAGTGATATACCGCAT
AGCGGTAATAATTGTGGCGTTTTTCTCTGGTCTTTCTAAAGATATAATGGCTATTGCCAATATCGGTGGAACTTTATTCT
CTTGTTTAGCTCTCATCAATATGACAGCGCTTATACTGTTTAGTAATCAGATTAACGAGGAAGTTCAGTGCTACCTAAAA
AGGCTGAGGAATAATAAAATCAATTAA

Upstream 100 bases:

>100_bases
TTTATGTGCCTTATGATTCCAAATGCTGCTGCAATATATCTACTAAGAAGGGAAGTTTTGAATACAATAGATTCTTATTA
CAATTCTAAAAGGTATTAAC

Downstream 100 bases:

>100_bases
ATTTTGGCATATGCGGAGAGGTATAGCTATAATAAGTAAGATTTCCCTACGTCATACCGCGATTTATTCGCGGTATCTCA
GCATAGATCCCGCTAACAAG

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MIYRLLCMVLFCLLFNDVYAASGFYEGYDAVLNGINNFMNEVLFFKIFYIPFILLLLSFGYVFLTLRFKFLNIRMFKHAF
AILCGKYDTNHHDGHITHFQAFMTALSSTVGLGTVAGVAIAVSMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEDQD
QLFSGPFQYIRNGLEEFGFKKLGIVLAAMYAVFFVLSGLGGSVAFQTNQMVSILSGYSSWVDGHSWFLSSIISALLALVI
IGGIKRIARVSSALVPIMSLIYIFSCIIIIAFNIHNLGYTLKILFSTMIDFNAVGGGMVGAFVAGIQRAIFASEAGVGSA
SITHATTKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTSVGEGILITQKAFETVSPWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGAKSIVIYRIAVIIVAFFSGLSKDIMAIANIGGTLFSCLALINMTALILFSNQINEEVQCYLK
RLRNNKIN

Sequences:

>Translated_488_residues
MIYRLLCMVLFCLLFNDVYAASGFYEGYDAVLNGINNFMNEVLFFKIFYIPFILLLLSFGYVFLTLRFKFLNIRMFKHAF
AILCGKYDTNHHDGHITHFQAFMTALSSTVGLGTVAGVAIAVSMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEDQD
QLFSGPFQYIRNGLEEFGFKKLGIVLAAMYAVFFVLSGLGGSVAFQTNQMVSILSGYSSWVDGHSWFLSSIISALLALVI
IGGIKRIARVSSALVPIMSLIYIFSCIIIIAFNIHNLGYTLKILFSTMIDFNAVGGGMVGAFVAGIQRAIFASEAGVGSA
SITHATTKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTSVGEGILITQKAFETVSPWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGAKSIVIYRIAVIIVAFFSGLSKDIMAIANIGGTLFSCLALINMTALILFSNQINEEVQCYLK
RLRNNKIN
>Mature_488_residues
MIYRLLCMVLFCLLFNDVYAASGFYEGYDAVLNGINNFMNEVLFFKIFYIPFILLLLSFGYVFLTLRFKFLNIRMFKHAF
AILCGKYDTNHHDGHITHFQAFMTALSSTVGLGTVAGVAIAVSMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEDQD
QLFSGPFQYIRNGLEEFGFKKLGIVLAAMYAVFFVLSGLGGSVAFQTNQMVSILSGYSSWVDGHSWFLSSIISALLALVI
IGGIKRIARVSSALVPIMSLIYIFSCIIIIAFNIHNLGYTLKILFSTMIDFNAVGGGMVGAFVAGIQRAIFASEAGVGSA
SITHATTKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTSVGEGILITQKAFETVSPWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGAKSIVIYRIAVIIVAFFSGLSKDIMAIANIGGTLFSCLALINMTALILFSNQINEEVQCYLK
RLRNNKIN

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=461, Percent_Identity=28.8503253796095, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 53521; Mature: 53521

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCC
>Mature Secondary Structure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HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]