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The map label for this gene is purK [H]

Identifier: 225630703

GI number: 225630703

Start: 1069272

End: 1070336

Strand: Reverse

Name: purK [H]

Synonym: WRi_009960

Alternate gene names: 225630703

Gene position: 1070336-1069272 (Counterclockwise)

Preceding gene: 225630706

Following gene: 225630701

Centisome position: 74.03

GC content: 35.12

Gene sequence:

>1065_bases
ATGAATGAACCAGATGCGCTTAGCAAAAAAGTAATAGGAATAATAGGTGGTGGACAATTAGGTAAAATGACTGCTATCGC
TGCAACAAAACTTGGACAAAAAACACATGTTTTTGCCAGTGCTAAAGACGATCCGGCTTGCTCTGTTGCTGATGATTTCA
CAATAGCAGATTTCTCTGATAAGAAAGCTCTTGAATCTTTTGCACAGAGTGTGGATTTAGTTACTATTGAGTCTGAAAAT
ATTCCATGTAGTGCAATTGATATCGATGTAAATTTTTATCCGGGTAAAAAAGCGTTACACATTTCGCAGAACAGACTGAG
AGAAAAGGATTTTATTAAAAACTTGGGTATAAAAACTGCTAACTATAAGAGTATACAAAATTATAATGAGCTACTGAAAA
GCAGTAGAGCTTTTGGCTATCCAACAAGGCTGAAAACAACAGAAATGGGTTATGATGGAAAAGGGCAATATGTGCTTGAG
AATGATTCTGAAGTGAAGCAATTTGCTTCCTTTGATTGGAATACAGAGTACATTCTTGAAGCAAGTGTTGATTTACTGAA
AGAGGTTTCAATAGTCGTTGCAAGAGATAAAAACGGTAAAGTAGCTTTTTTTCCTATAGCAGAAAATTACCACGTTGATG
GAATACTTGATACTTCAACAGTGCCAGCTAAAATAGATAGTAAATTAACTCAAGAGGTACAACGAGCTGCAAAGAAAATA
GCAAATGCGCTTGATGTAATAGGAATTCTGGCTATTGAATTTTTTGTTACTAAGGATAACGAATTGTTAGTTAATGAACT
AGCTCCCAGACCTCACAATTCTTGCCACTGGAGCTTGGATGCATGTAACGTTAGTCAATTTGAACAACTGGTTAGGATAA
TATGCGGGCTACCTATGCAGGAAGTAGTATTACGCTTTCCTTGTATGACGAAAAATATAATAGGTAATGATATATATGAT
TCTCATAAGTATTTGAGCAACGAAAAAGCTAGTTTAACCATATATGGGAAAAAAGAGGTTAGGGATAAGCGTAAAATGGG
ACATGTCAATATAGATTTAAGTTAA

Upstream 100 bases:

>100_bases
TCATTATAAAATATTCATGATAAGAAAATAGTATCTTAATTATATACTTTTTTCATAGATTTTTCAAGTATACTTTAATT
AGGAAGTATCAATTTATAGT

Downstream 100 bases:

>100_bases
CAGTTTTTCTACGTCATACCGCCACAACTGTACGAACATTGTGATATGATGAGATGTTACATAGTAAACGGTGTCATTCT
AGCACCTCTTTTCCCGTCAT

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNEPDALSKKVIGIIGGGQLGKMTAIAATKLGQKTHVFASAKDDPACSVADDFTIADFSDKKALESFAQSVDLVTIESEN
IPCSAIDIDVNFYPGKKALHISQNRLREKDFIKNLGIKTANYKSIQNYNELLKSSRAFGYPTRLKTTEMGYDGKGQYVLE
NDSEVKQFASFDWNTEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENYHVDGILDTSTVPAKIDSKLTQEVQRAAKKI
ANALDVIGILAIEFFVTKDNELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLPMQEVVLRFPCMTKNIIGNDIYD
SHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLS

Sequences:

>Translated_354_residues
MNEPDALSKKVIGIIGGGQLGKMTAIAATKLGQKTHVFASAKDDPACSVADDFTIADFSDKKALESFAQSVDLVTIESEN
IPCSAIDIDVNFYPGKKALHISQNRLREKDFIKNLGIKTANYKSIQNYNELLKSSRAFGYPTRLKTTEMGYDGKGQYVLE
NDSEVKQFASFDWNTEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENYHVDGILDTSTVPAKIDSKLTQEVQRAAKKI
ANALDVIGILAIEFFVTKDNELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLPMQEVVLRFPCMTKNIIGNDIYD
SHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLS
>Mature_354_residues
MNEPDALSKKVIGIIGGGQLGKMTAIAATKLGQKTHVFASAKDDPACSVADDFTIADFSDKKALESFAQSVDLVTIESEN
IPCSAIDIDVNFYPGKKALHISQNRLREKDFIKNLGIKTANYKSIQNYNELLKSSRAFGYPTRLKTTEMGYDGKGQYVLE
NDSEVKQFASFDWNTEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENYHVDGILDTSTVPAKIDSKLTQEVQRAAKKI
ANALDVIGILAIEFFVTKDNELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLPMQEVVLRFPCMTKNIIGNDIYD
SHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLS

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=345, Percent_Identity=31.5942028985507, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788155, Length=352, Percent_Identity=23.2954545454545, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=31.4207650273224, Blast_Score=189, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39394; Mature: 39394

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPDALSKKVIGIIGGGQLGKMTAIAATKLGQKTHVFASAKDDPACSVADDFTIADFSD
CCCCHHHHCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCH
KKALESFAQSVDLVTIESENIPCSAIDIDVNFYPGKKALHISQNRLREKDFIKNLGIKTA
HHHHHHHHHCCEEEEEECCCCCEEEEEEEEEEECCCEEEEECHHHHHHHHHHHHCCCCCC
NYKSIQNYNELLKSSRAFGYPTRLKTTEMGYDGKGQYVLENDSEVKQFASFDWNTEYILE
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEECCHHHHHHHCCCCCCEEEEH
ASVDLLKEVSIVVARDKNGKVAFFPIAENYHVDGILDTSTVPAKIDSKLTQEVQRAAKKI
HHHHHHHHEEEEEEECCCCCEEEEEECCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHH
ANALDVIGILAIEFFVTKDNELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLPMQ
HHHHHHHHHHHEEEEEECCCCEEHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHH
EVVLRFPCMTKNIIGNDIYDSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLS
HHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MNEPDALSKKVIGIIGGGQLGKMTAIAATKLGQKTHVFASAKDDPACSVADDFTIADFSD
CCCCHHHHCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCH
KKALESFAQSVDLVTIESENIPCSAIDIDVNFYPGKKALHISQNRLREKDFIKNLGIKTA
HHHHHHHHHCCEEEEEECCCCCEEEEEEEEEEECCCEEEEECHHHHHHHHHHHHCCCCCC
NYKSIQNYNELLKSSRAFGYPTRLKTTEMGYDGKGQYVLENDSEVKQFASFDWNTEYILE
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEECCHHHHHHHCCCCCCEEEEH
ASVDLLKEVSIVVARDKNGKVAFFPIAENYHVDGILDTSTVPAKIDSKLTQEVQRAAKKI
HHHHHHHHEEEEEEECCCCCEEEEEECCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHH
ANALDVIGILAIEFFVTKDNELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLPMQ
HHHHHHHHHHHEEEEEECCCCEEHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHH
EVVLRFPCMTKNIIGNDIYDSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLS
HHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]