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The map label for this gene is purC [H]

Identifier: 225630686

GI number: 225630686

Start: 1052547

End: 1053269

Strand: Direct

Name: purC [H]

Synonym: WRi_009790

Alternate gene names: 225630686

Gene position: 1052547-1053269 (Clockwise)

Preceding gene: 225630680

Following gene: 225630688

Centisome position: 72.8

GC content: 31.4

Gene sequence:

>723_bases
ATGTCACTGAATAAAACGATATACGAAGGTAAAGCGAAAGCCATTATTGAAACTGAAGACTCATCAACTGTTATACAGCA
CTTTAAAGATGATGTTACAGCATTCAATAAGGAAAAATATGAAATTATTGAGAGCAAAGGAATAATTAATAATCATATTA
GTGCTTTTATCATGGAAAAGCTTGAGAAAGCAGAAATTAGCACACACTTTATAAAAACTCTAAACGAAAGAGAACAGCTA
GTCAAAAAACTCAAGATAATACCTCTTGAGGTAGTAGTTAGAAATGTCGCAGCTGGCAGCTTTTGCAAACGTTTTAATAT
CAAAGAAGGTGAGAGGCTTGCATCTCCTATAATCGATTTTTTTTACAAAAATGATGACCTAGCCGACCCAATGGTAAGTG
AAAATCACATACTGTATTTTGATTGGCTATCTAGCAAAGAAATGGATGAAGTTAAAACTACAACTTTAAAAATCAACGAA
ATATTAGTTCACCTATTTTCAAATGCAAGTATATATTTAGTTGATCTCAAGTTAGAATTTGGCAGATTAATAAATGACAG
TACTAAAATCGTTTTAGCTGATGAAATTAGCCCTGATAACTGCAGATTATGGGATAAAAATACTTATAAAAAGCTAGATA
AAGATGTCTTTCGTTTGAATTTAGGAGATTTAAAGGAAGCATACCTAGAAGTTGCAAAAAGACTGTCAGTAAAGTTAGGT
TAA

Upstream 100 bases:

>100_bases
GTTAATATTGTAACATTGAATGCCATTAAGGTTTATTTTAAGTTATTTGGGGCTATACTCTAAGAATAGTAAATTGATAT
AATATATAGTTAAATTAAAC

Downstream 100 bases:

>100_bases
CTAAATACTACTCTATCAAGTGGAGGGTTACATGCCTCATCAAGTTTTCCAATAATTTCAATTTTTTCTCCACGTTTTTG
GAAATGATTTTTCACGTTTT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSLNKTIYEGKAKAIIETEDSSTVIQHFKDDVTAFNKEKYEIIESKGIINNHISAFIMEKLEKAEISTHFIKTLNEREQL
VKKLKIIPLEVVVRNVAAGSFCKRFNIKEGERLASPIIDFFYKNDDLADPMVSENHILYFDWLSSKEMDEVKTTTLKINE
ILVHLFSNASIYLVDLKLEFGRLINDSTKIVLADEISPDNCRLWDKNTYKKLDKDVFRLNLGDLKEAYLEVAKRLSVKLG

Sequences:

>Translated_240_residues
MSLNKTIYEGKAKAIIETEDSSTVIQHFKDDVTAFNKEKYEIIESKGIINNHISAFIMEKLEKAEISTHFIKTLNEREQL
VKKLKIIPLEVVVRNVAAGSFCKRFNIKEGERLASPIIDFFYKNDDLADPMVSENHILYFDWLSSKEMDEVKTTTLKINE
ILVHLFSNASIYLVDLKLEFGRLINDSTKIVLADEISPDNCRLWDKNTYKKLDKDVFRLNLGDLKEAYLEVAKRLSVKLG
>Mature_239_residues
SLNKTIYEGKAKAIIETEDSSTVIQHFKDDVTAFNKEKYEIIESKGIINNHISAFIMEKLEKAEISTHFIKTLNEREQLV
KKLKIIPLEVVVRNVAAGSFCKRFNIKEGERLASPIIDFFYKNDDLADPMVSENHILYFDWLSSKEMDEVKTTTLKINEI
LVHLFSNASIYLVDLKLEFGRLINDSTKIVLADEISPDNCRLWDKNTYKKLDKDVFRLNLGDLKEAYLEVAKRLSVKLG

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=257, Percent_Identity=33.852140077821, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI5453539, Length=257, Percent_Identity=33.852140077821, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI119220559, Length=264, Percent_Identity=32.9545454545455, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1788820, Length=239, Percent_Identity=45.1882845188285, Blast_Score=211, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17531275, Length=229, Percent_Identity=33.1877729257642, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18860083, Length=241, Percent_Identity=33.195020746888, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24583917, Length=241, Percent_Identity=27.8008298755187, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 27681; Mature: 27550

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNKTIYEGKAKAIIETEDSSTVIQHFKDDVTAFNKEKYEIIESKGIINNHISAFIMEK
CCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
LEKAEISTHFIKTLNEREQLVKKLKIIPLEVVVRNVAAGSFCKRFNIKEGERLASPIIDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FYKNDDLADPMVSENHILYFDWLSSKEMDEVKTTTLKINEILVHLFSNASIYLVDLKLEF
HHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHH
GRLINDSTKIVLADEISPDNCRLWDKNTYKKLDKDVFRLNLGDLKEAYLEVAKRLSVKLG
HHHCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLNKTIYEGKAKAIIETEDSSTVIQHFKDDVTAFNKEKYEIIESKGIINNHISAFIMEK
CCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
LEKAEISTHFIKTLNEREQLVKKLKIIPLEVVVRNVAAGSFCKRFNIKEGERLASPIIDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FYKNDDLADPMVSENHILYFDWLSSKEMDEVKTTTLKINEILVHLFSNASIYLVDLKLEF
HHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHH
GRLINDSTKIVLADEISPDNCRLWDKNTYKKLDKDVFRLNLGDLKEAYLEVAKRLSVKLG
HHHCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA