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The map label for this gene is lipB [H]

Identifier: 225630679

GI number: 225630679

Start: 1044899

End: 1045516

Strand: Direct

Name: lipB [H]

Synonym: WRi_009720

Alternate gene names: 225630679

Gene position: 1044899-1045516 (Clockwise)

Preceding gene: 225630676

Following gene: 225630680

Centisome position: 72.27

GC content: 33.82

Gene sequence:

>618_bases
ATGACAGAATGGCTAATATCTAATCAGCTTATTGATTATAACTGTGCCGTAAAATCTATGGAAGAGAAAATTCAACAAAT
TCACAATAATTCAGCAGACGAATTAGTATGGCTACTCCAGCACCCTCCACTTTATACTGCAGGAATCAGTGCAACAGATG
ACGACGTTGTTGAAAAATTATTTCCTATATATAAAACAGGCAGGGGTGGCAAATACACATATCATGGTCCAGGACAGCGC
ATCATATATTTAATGTTAAACCTTAAAAAAAGAAATAAATGCGACATAAAGCTATATATTAGAGACCTAAGTAAGTGGAT
AATAAATGTTTTAAAGCAATTTAATATACTTGGAGAATTTAAAGAGGATAGAATAGGTGTTTGGGTGAATAACAATGGAG
TAGAAAAAAAAATTGCAGCTTTTGGTATCCGTTTAAGAAAATGGGTAACTTATCATGGCATAGCGCTTAACGTCTTTCCA
GACCTCTCTCATTATAAGGGTATTATTCCTTGTGGACTGCAAGATTATGGCGTCACGTCAATGGAAGAACTAGGAGTGAA
AGTTCCACTCTCTGAATTGGATGATATACTAAAAAAAGAGTTTTATAAGATATTTTAA

Upstream 100 bases:

>100_bases
TATTAAGTATATTAGGAATAAGAAAGGCTGCGCCCAGTGTCACACACTGGGATGACAGGAAAAGAACGCACTGCTATACT
AAAAAGAAAAGAAATTGAAC

Downstream 100 bases:

>100_bases
TGATTGTTTATCTTTTATATTTCATTCTCACTTTATGTGCTAACACAAGTATTCATGCAATCGACCAAGTTGATATTATT
GCTCCTTCCTCCAAGGGAAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATDDDVVEKLFPIYKTGRGGKYTYHGPGQR
IIYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEFKEDRIGVWVNNNGVEKKIAAFGIRLRKWVTYHGIALNVFP
DLSHYKGIIPCGLQDYGVTSMEELGVKVPLSELDDILKKEFYKIF

Sequences:

>Translated_205_residues
MTEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATDDDVVEKLFPIYKTGRGGKYTYHGPGQR
IIYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEFKEDRIGVWVNNNGVEKKIAAFGIRLRKWVTYHGIALNVFP
DLSHYKGIIPCGLQDYGVTSMEELGVKVPLSELDDILKKEFYKIF
>Mature_204_residues
TEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATDDDVVEKLFPIYKTGRGGKYTYHGPGQRI
IYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEFKEDRIGVWVNNNGVEKKIAAFGIRLRKWVTYHGIALNVFPD
LSHYKGIIPCGLQDYGVTSMEELGVKVPLSELDDILKKEFYKIF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=193, Percent_Identity=29.0155440414508, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87081767, Length=164, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323268, Length=125, Percent_Identity=36, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355483, Length=192, Percent_Identity=34.375, Blast_Score=106, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23740; Mature: 23609

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATDDDVVEKL
CCCCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCCCCCCHHHHHHH
FPIYKTGRGGKYTYHGPGQRIIYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEF
HHHHCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
KEDRIGVWVNNNGVEKKIAAFGIRLRKWVTYHGIALNVFPDLSHYKGIIPCGLQDYGVTS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCC
MEELGVKVPLSELDDILKKEFYKIF
HHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATDDDVVEKL
CCCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCCCCCCHHHHHHH
FPIYKTGRGGKYTYHGPGQRIIYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEF
HHHHCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
KEDRIGVWVNNNGVEKKIAAFGIRLRKWVTYHGIALNVFPDLSHYKGIIPCGLQDYGVTS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCC
MEELGVKVPLSELDDILKKEFYKIF
HHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA