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The map label for this gene is iscS [H]

Identifier: 225630661

GI number: 225630661

Start: 1016780

End: 1018027

Strand: Reverse

Name: iscS [H]

Synonym: WRi_009450

Alternate gene names: 225630661

Gene position: 1018027-1016780 (Counterclockwise)

Preceding gene: 225630662

Following gene: 225630651

Centisome position: 70.41

GC content: 38.46

Gene sequence:

>1248_bases
ATGAATGTTGAAAGTAAAAAAATACACGATGCTAAAATAAAATTACCAATATTTCTTGATTATCAATCTACTACTAAAGT
AGATCCCCGTGTTTTAGAGGTGATGATACCTTACTTTGGTGAGTTCAGCAATGCACACTCTCGCAGCCATTCATTTGGTT
GGACTGCTGAAGAAGCAGTGGAAAAAGCAAGGAAACACATCGCTGATCTGGTGAACGCAGATAGCAAAGAAATTATCTTT
ACCTCAGGTGCAACTGAGTCAAATAATACGGCAATCAAGGGTGTTGCTCACTTTTATAAAAATAAAGGAAATCATATAAT
AACTGTCTGCACGGAGCATAAGTGTGTTCTGGATTCTTGTCGGCATCTGGAAAATGAAGGCTTTAAAGTTACATATTTGC
CTGTTAAGCAAAACGGAATTATAGATTTATCAAAGCTTGAGGAAGCAATTACTGATCAAACAATCCTGGTTTCAGTGATG
ATGGTAAATAATGAAATCGGAGTAATTCAGCCTGTCAAAGAAATTGGTGTAATATGTAGAAAGCATAATATATTTTTCCA
CACAGATGCAGCTCAAAGCTTTGGAAAAGTTCCAATTGATGTTAATGAGATGTGCATTGATCTTATGAGTCTCTCTAGCC
ACAAAATTTATGGACCTATGGGAATAGGCGCATTATATGTACGTAGAAAGAATCCTCGTGTTAGGCTGATACCACTAATT
AGTGGTGGTGGACAGGAAAGAGGCATGCGTTCTGGAACAGTTCCAACACCTCTTGCAGTTGGTTTTGGTGAAGCGGCACG
TATTGCTAGAGAAGAAATGAAAAAAGAAACAACTAGAGTGGAAGAATTAAGGGATATTTTGTATAACAAGATAAAAGAAG
CGTTTCCTGATATCGTTTTAAATGGTGATTATGAAAATAGAATTCCTGGCAACCTAAACTTGAGCTTTCCCTATATTGAG
GGTGAGTCTCTCATCATGGCAATCAAGGATTTAGCAGTAAGTTCTGGTTCTGCGTGCACGTCTGCGTCTCTTGAGCCTTC
CTATGTAGTACGGTCGCTAAATAATGGCCATGACCTTGAGCATTCATCAATTAGATTTGGCCTAGGCCGATTTACAACTA
AAGAAGAAGTTTTGTATGCAGCAGATCTTGTTGCTAAAAATGTTGGCCGACTGAGAGAAATGAGCCCACTTTGGGAGATG
GTACAAGAAGGAGTAGATTTGAACACCGTGAAATGGGACTCTCATTAA

Upstream 100 bases:

>100_bases
ACTTGGATGACACTTTTGTACTTGATGCAAGATTATATCTTGAAAAAATTAAGTTTTAATGCTATAAAATAATTGTAAAT
TTTGTCTCCGTGGTAGCTTT

Downstream 100 bases:

>100_bases
ATGTATCCGTTCCAACTGCAAATGCACTTTGAAATTACTCTGTACCGTTCACACTTAATTCTTCAATCTCACTAATAGAA
AGGCCAGTAAACTTGACAAT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVESKKIHDAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIIF
TSGATESNNTAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLPVKQNGIIDLSKLEEAITDQTILVSVM
MVNNEIGVIQPVKEIGVICRKHNIFFHTDAAQSFGKVPIDVNEMCIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLIPLI
SGGGQERGMRSGTVPTPLAVGFGEAARIAREEMKKETTRVEELRDILYNKIKEAFPDIVLNGDYENRIPGNLNLSFPYIE
GESLIMAIKDLAVSSGSACTSASLEPSYVVRSLNNGHDLEHSSIRFGLGRFTTKEEVLYAADLVAKNVGRLREMSPLWEM
VQEGVDLNTVKWDSH

Sequences:

>Translated_415_residues
MNVESKKIHDAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIIF
TSGATESNNTAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLPVKQNGIIDLSKLEEAITDQTILVSVM
MVNNEIGVIQPVKEIGVICRKHNIFFHTDAAQSFGKVPIDVNEMCIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLIPLI
SGGGQERGMRSGTVPTPLAVGFGEAARIAREEMKKETTRVEELRDILYNKIKEAFPDIVLNGDYENRIPGNLNLSFPYIE
GESLIMAIKDLAVSSGSACTSASLEPSYVVRSLNNGHDLEHSSIRFGLGRFTTKEEVLYAADLVAKNVGRLREMSPLWEM
VQEGVDLNTVKWDSH
>Mature_415_residues
MNVESKKIHDAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIIF
TSGATESNNTAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLPVKQNGIIDLSKLEEAITDQTILVSVM
MVNNEIGVIQPVKEIGVICRKHNIFFHTDAAQSFGKVPIDVNEMCIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLIPLI
SGGGQERGMRSGTVPTPLAVGFGEAARIAREEMKKETTRVEELRDILYNKIKEAFPDIVLNGDYENRIPGNLNLSFPYIE
GESLIMAIKDLAVSSGSACTSASLEPSYVVRSLNNGHDLEHSSIRFGLGRFTTKEEVLYAADLVAKNVGRLREMSPLWEM
VQEGVDLNTVKWDSH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=401, Percent_Identity=61.8453865336658, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI156713448, Length=408, Percent_Identity=33.0882352941176, Blast_Score=205, Evalue=8e-53,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=62.807881773399, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787970, Length=234, Percent_Identity=27.3504273504274, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789175, Length=230, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=402, Percent_Identity=63.18407960199, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=32.0121951219512, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=60.5985037406484, Blast_Score=508, Evalue=1e-145,
Organism=Drosophila melanogaster, GI20129463, Length=401, Percent_Identity=65.5860349127182, Blast_Score=554, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 46277; Mature: 46277

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVESKKIHDAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAV
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
EKARKHIADLVNADSKEIIFTSGATESNNTAIKGVAHFYKNKGNHIITVCTEHKCVLDSC
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
RHLENEGFKVTYLPVKQNGIIDLSKLEEAITDQTILVSVMMVNNEIGVIQPVKEIGVICR
HHHCCCCEEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHE
KHNIFFHTDAAQSFGKVPIDVNEMCIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLIPLI
ECCEEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCEEEEEEE
SGGGQERGMRSGTVPTPLAVGFGEAARIAREEMKKETTRVEELRDILYNKIKEAFPDIVL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NGDYENRIPGNLNLSFPYIEGESLIMAIKDLAVSSGSACTSASLEPSYVVRSLNNGHDLE
CCCCCCCCCCCEEEEECEECCCHHEEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCC
HSSIRFGLGRFTTKEEVLYAADLVAKNVGRLREMSPLWEMVQEGVDLNTVKWDSH
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MNVESKKIHDAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAV
CCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
EKARKHIADLVNADSKEIIFTSGATESNNTAIKGVAHFYKNKGNHIITVCTEHKCVLDSC
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
RHLENEGFKVTYLPVKQNGIIDLSKLEEAITDQTILVSVMMVNNEIGVIQPVKEIGVICR
HHHCCCCEEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHE
KHNIFFHTDAAQSFGKVPIDVNEMCIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLIPLI
ECCEEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCEEEEEEE
SGGGQERGMRSGTVPTPLAVGFGEAARIAREEMKKETTRVEELRDILYNKIKEAFPDIVL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NGDYENRIPGNLNLSFPYIEGESLIMAIKDLAVSSGSACTSASLEPSYVVRSLNNGHDLE
CCCCCCCCCCCEEEEECEECCCHHEEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCC
HSSIRFGLGRFTTKEEVLYAADLVAKNVGRLREMSPLWEMVQEGVDLNTVKWDSH
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA