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The map label for this gene is ccmE

Identifier: 225630639

GI number: 225630639

Start: 991735

End: 992136

Strand: Direct

Name: ccmE

Synonym: WRi_009180

Alternate gene names: 225630639

Gene position: 991735-992136 (Clockwise)

Preceding gene: 225630638

Following gene: 225630643

Centisome position: 68.59

GC content: 32.09

Gene sequence:

>402_bases
ATGAAGAAGAAACATAAGCGATTACTTATAACTTCAGGAATTTTCTGCTTTTTAAGCTGCATAGTCTTTTTTATTTTAAC
AACGCTCAAAGAAAACATCTCATTTTTCTATACAGTAAGTGAGGCAATAGTTTTACAAAATAGTCAAAAACTAATCCGTG
TTGGTGGAATGGTTGTTGAAAATAGCGTGATACGGAGCGAAAGTGAAGTAATTTTTCAAATGACAGATTTTAACAAAAGC
GTTGTGGTAAAATATCAAGGGATACTTCCACCAATGTTTTCAGAAAAAAGTGGTGTTGTTGTGCAAGGTAAAATGTTTGA
TAATAGTACTTTTCTTGCAGATACAGTGTTTGCAAAACATGATGAGAACTATATGCCGAAAGTTTTAAAACAGATTCCCT
AA

Upstream 100 bases:

>100_bases
GCTTACCTTATTAGTCTTACATTGATTACCGGAGAACTGATTCTTACCATTTCTTGCTATATTAAAAGTAAAAAAATTCT
AGAAAGCCTGAAAGATAATA

Downstream 100 bases:

>100_bases
TTCAGCTGTATTGAAACGATATGTAGAGAGAGTAACAGCCCACTGGGCTATTACTTTTTTTCTTATATGAATTCAGGAGC
ATCCATTGGTGGCGGTTCTA

Product: cytochrome c-type biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKKKHKRLLITSGIFCFLSCIVFFILTTLKENISFFYTVSEAIVLQNSQKLIRVGGMVVENSVIRSESEVIFQMTDFNKS
VVVKYQGILPPMFSEKSGVVVQGKMFDNSTFLADTVFAKHDENYMPKVLKQIP

Sequences:

>Translated_133_residues
MKKKHKRLLITSGIFCFLSCIVFFILTTLKENISFFYTVSEAIVLQNSQKLIRVGGMVVENSVIRSESEVIFQMTDFNKS
VVVKYQGILPPMFSEKSGVVVQGKMFDNSTFLADTVFAKHDENYMPKVLKQIP
>Mature_133_residues
MKKKHKRLLITSGIFCFLSCIVFFILTTLKENISFFYTVSEAIVLQNSQKLIRVGGMVVENSVIRSESEVIFQMTDFNKS
VVVKYQGILPPMFSEKSGVVVQGKMFDNSTFLADTVFAKHDENYMPKVLKQIP

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=133, Percent_Identity=39.0977443609023, Blast_Score=94, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_WOLWR (C0R3Z7)

Other databases:

- EMBL:   CP001391
- RefSeq:   YP_002727430.1
- ProteinModelPortal:   C0R3Z7
- EnsemblBacteria:   EBWOLT00000001460
- GeneID:   7669640
- GenomeReviews:   CP001391_GR
- KEGG:   wri:WRi_009180
- GeneTree:   EBGT00050000030966
- OMA:   ENISFFY
- ProtClustDB:   PRK13254
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 15153; Mature: 15153

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: BINDING 120-120

Signals:

None

Transmembrane regions:

HASH(0x1b7ff0d8)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHKRLLITSGIFCFLSCIVFFILTTLKENISFFYTVSEAIVLQNSQKLIRVGGMVVE
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHEEHHCCEEEE
NSVIRSESEVIFQMTDFNKSVVVKYQGILPPMFSEKSGVVVQGKMFDNSTFLADTVFAKH
CHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCEEHHHHHHHCC
DENYMPKVLKQIP
CCCHHHHHHHHCH
>Mature Secondary Structure
MKKKHKRLLITSGIFCFLSCIVFFILTTLKENISFFYTVSEAIVLQNSQKLIRVGGMVVE
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHEEHHCCEEEE
NSVIRSESEVIFQMTDFNKSVVVKYQGILPPMFSEKSGVVVQGKMFDNSTFLADTVFAKH
CHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCEEHHHHHHHCC
DENYMPKVLKQIP
CCCHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA