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The map label for this gene is nuoJ [H]

Identifier: 225630632

GI number: 225630632

Start: 985173

End: 985760

Strand: Direct

Name: nuoJ [H]

Synonym: WRi_009110

Alternate gene names: 225630632

Gene position: 985173-985760 (Clockwise)

Preceding gene: 225630629

Following gene: 225630633

Centisome position: 68.14

GC content: 35.2

Gene sequence:

>588_bases
ATGCCTTTTTTCTTTTATTTTTTTTCGATTTTGAGCATTTTATCTGCTGTTTGTGTGATTAGCGTAAGAAATCCTGTGCA
TGCAGTATTATTTTTAATTTTCACCTTCGTTAACTCTGCAGTGCTTTTTATTCTCCTTGGAGCTGAATTCATTGCTATGA
TGGTGCTGATAGTATACATCGGCGCGGTTGCAGTGTTATTCCTCTTTGTAGTTATGATGCTCGATATTGACTACATAAGG
TTGCGCCAGGGTTTTGCAAAGCATTTCACTCTTGGTGCTATATTATGTGTTGTGTTTTTTCTAGTCATCAGCTTTGTAAT
CCGCAGCTCAACGCTGAATATAAGTAATGTTATAAACTATAATACTAATAACGTGAAAGCTATTGGTAATTTGCTGTATA
CTGACTACATGTACGCTTTTCATCTTTCTGGTATTCTGCTGCTTGTTGCAATTGTCGGTGCAATTGCTCTTACTTTGCAA
GACAAGAAAAAAGGAATTAAAAGACAGAATGTTTTAAAGCAATTGACGCAATCTTCATCTGTAAAATTGGTTAAGGCTAA
ATTTGGAAAAGGAGTAGAATGGAAATAG

Upstream 100 bases:

>100_bases
TGGAGCTTTGTAGTACAATGTTTTCAAACGTTTAATATAATATTGACTTTACAACAATAGTCACTTTAAATTAGTAACTA
TTGTTGCAATTAAAGTATCA

Downstream 100 bases:

>100_bases
GATTAAATCATTTTCTGATAGTTGCTGCTGTTTTGTTCACTATTGGGGTGTGCGGTATTTTCATTAACCGTAAGAGCATA
ATCAACATACTGTTATCAAT

Product: NADH dehydrogenase I, J subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit J; NDH-1 subunit J [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MPFFFYFFSILSILSAVCVISVRNPVHAVLFLIFTFVNSAVLFILLGAEFIAMMVLIVYIGAVAVLFLFVVMMLDIDYIR
LRQGFAKHFTLGAILCVVFFLVISFVIRSSTLNISNVINYNTNNVKAIGNLLYTDYMYAFHLSGILLLVAIVGAIALTLQ
DKKKGIKRQNVLKQLTQSSSVKLVKAKFGKGVEWK

Sequences:

>Translated_195_residues
MPFFFYFFSILSILSAVCVISVRNPVHAVLFLIFTFVNSAVLFILLGAEFIAMMVLIVYIGAVAVLFLFVVMMLDIDYIR
LRQGFAKHFTLGAILCVVFFLVISFVIRSSTLNISNVINYNTNNVKAIGNLLYTDYMYAFHLSGILLLVAIVGAIALTLQ
DKKKGIKRQNVLKQLTQSSSVKLVKAKFGKGVEWK
>Mature_194_residues
PFFFYFFSILSILSAVCVISVRNPVHAVLFLIFTFVNSAVLFILLGAEFIAMMVLIVYIGAVAVLFLFVVMMLDIDYIRL
RQGFAKHFTLGAILCVVFFLVISFVIRSSTLNISNVINYNTNNVKAIGNLLYTDYMYAFHLSGILLLVAIVGAIALTLQD
KKKGIKRQNVLKQLTQSSSVKLVKAKFGKGVEWK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0839

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 6 (chain J)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 6 family [H]

Homologues:

Organism=Escherichia coli, GI1788616, Length=188, Percent_Identity=31.9148936170213, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001457 [H]

Pfam domain/function: PF00499 Oxidored_q3 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21835; Mature: 21704

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFFFYFFSILSILSAVCVISVRNPVHAVLFLIFTFVNSAVLFILLGAEFIAMMVLIVYI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVAVLFLFVVMMLDIDYIRLRQGFAKHFTLGAILCVVFFLVISFVIRSSTLNISNVINY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
NTNNVKAIGNLLYTDYMYAFHLSGILLLVAIVGAIALTLQDKKKGIKRQNVLKQLTQSSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VKLVKAKFGKGVEWK
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
PFFFYFFSILSILSAVCVISVRNPVHAVLFLIFTFVNSAVLFILLGAEFIAMMVLIVYI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVAVLFLFVVMMLDIDYIRLRQGFAKHFTLGAILCVVFFLVISFVIRSSTLNISNVINY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
NTNNVKAIGNLLYTDYMYAFHLSGILLLVAIVGAIALTLQDKKKGIKRQNVLKQLTQSSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
VKLVKAKFGKGVEWK
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]